Crops ›› 2016, Vol. 32 ›› Issue (6): 9-15.doi: 10.16035/j.issn.1001-7283.2016.06.002

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Role and Expression Regulation of Phytocystatin in Plant Resistance to Diseases and Pests

Yu Yongting,Gao Chunsheng,Li Zhimin,Zeng Liangbin,Chen Jia,Zhu Aiguo,Zhu Taotao,Sun Kai,Yan Zhun   

  1. Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,Hunan,China
  • Received:2016-08-19 Revised:2016-11-01 Online:2016-12-15 Published:2018-08-26

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Abstract:

Plant cysteine proteinase inhibitor, also called phytocystatin, plays a critical role in plant resistance to bioticstress, such as nematode, herbivore and fungi, etc. It can impair their growth and reproduction by directly inhibiting the activity of cysteine proteinase, an important digestive enzyme of nematode or herbivore when they infect plant. However, the impairing function of phytocystatin to some phytopathogenic fungi does not depend on its cysteine proteinase inhibiting activity and its underlying mechanism is still unclear. When plant suffered to biotic stress, expression of phytocystatin is regulated by various genes and is closely related to JA signaling. However, regulation network of phytocystatin expression is unclear and need more studies to reveal. This review summarizes the recent studies highlighting the role and application of phytocystatin in plant resistance to nematode and herbivore, and analyzes the expression regulation mechanism of phytocystatin. In addition, we forecast the research tendency and application prospect of this protein.

Key words: Phytocystatin, Antifungal mechanism, Expression regulation, Disease-resistant, Pest-resistant

Fig.1

Distribution of the conserved sequences of PhytoCys on peptide chain"

Table 1

Some PhytoCys and its antifungal spectrum"

植物Species 蛋白名称Protein name 测试病原真菌Pathogen tested 参考文献Reference
板栗Chestnut CSC 禾生炭疽菌（Colletotrichum graminicola） [21]
灰葡萄孢（Botrytis cinerea）
颖枯壳针孢（Septoria nodorum）
甘蔗Sugarcane Canecystatin 里氏木霉（Trichoderma reesei） [22]
芋头Taro CeCPI 齐整小核菌（Sclerotium rolfsii） [18]
立枯丝核菌（Rhizoctonia solani）
芸薹链格孢（Alternaria brassicae）
围小丛壳菌（Glomerella cingulata）
瓜果腐霉（Pythium aphanidermatum）
尖孢镰孢（Fusarium oxysporum）
小麦Wheat TaMDC1 雪霉微座孢（Microdochium nivale） [23]
大麦Barley Hv-CPI 灰葡萄孢（Botrytis cinerea） [24]
禾生刺盘孢（Colletotrichum graminicola）
黄瓜织球壳菌（Plectosphaerella cucumerina）
绿色木霉（Trichoderma viride）
HvCPI-2 灰葡萄孢（Botrytis cinerea） [25]
HvCPI-3 尖孢镰刀菌（Fusarium oxysporum）
草莓Strawberry FaCPI-1 灰葡萄孢（Botrytis cinerea） [26]
尖孢镰刀菌（Fusarium oxysporum）
苋菜Amaranth AhCPI 立枯丝核菌（Rhizoctonia solani） [19]
尖孢镰刀菌（Fusarium oxysporum）
白腐小核菌（Sclerotium cepivorum）
猕猴桃Kiwifruit KCPIs 灰葡萄孢（Botrytis cinerea） [27]
根状链格孢（Alternaria radicina）
暹罗郁金香Siam tulip CaCPI 尖孢镰刀菌（Fusarium oxysporum） [28]
灰梨孢（Pyricularia grisea）
辣椒刺盘孢（Colletotrichum capsici）
可可Cacao TcCys1,TcCys2,TcCys3,TcCys4 可可丛枝病菌（Moniliophthora perniciosa） [20]
芝麻Sesame SiCYS 里氏木霉（Trichoderma reesei） [29]
萨氏曲霉（Aspergillus sydowii）
芝麻长蠕孢（Helminthosporium sesamum）
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