Crops ›› 2019, Vol. 35 ›› Issue (1): 75-80.doi: 10.16035/j.issn.1001-7283.2019.01.012

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Hybrid Purity Identification of Fengyou No.10 by SSR Markers in Bassica napus

Junping He,Shufen Zhang,Jianping Wang,Dongfang Cai,Jinhua Cao,Yancheng Wen,Kun Hu,Lei Zhao,Dongguo Wang,Jiacheng Zhu   

  1. Institute of Industrial Crops, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Genetic Improvement, Zhengzhou 450002, Henan, China
  • Received:2018-08-09 Revised:2018-12-13 Online:2019-02-15 Published:2019-02-01
  • Contact: Jiacheng Zhu

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Abstract:

Variety purity is an important index of variety identification, which indicates the uniformity and stability. In order to establish the hybrid purity identification method of SSR markers for Fengyou No.10 in Brassica napus L., Fengyou No.10 and its parents were used as experimental materials. Fluorescent SSR markers and capillary electrophoresis detection technology were employed to screen the primers for hybrid purity identification of Fengyou No.10 from 128 pairs of SSR primers. Results showed that two pairs of SSR primers for hybrid purity identification of Fengyou No.10 were obtained. The hybrid purity of Fengyou No.10 detected by the two SSR markers was 85.64%, which was very closed to the field morphological identification result (87.18%), showing that the hybrid purity of Fengyou No.10 can be identified by the screened SSR markers.

Key words: Hybrid purity identification, SSR markers, Fengyou No.10, Brassica napus L.

Table 1

Primers sequence expected to be used as purity identification of hybrid Fengyou No.10"

引物
Primer		 5′-3′正向引物序列
Forward primer sequence of 5′-3′		 5′-3′反向引物序列
Reverse primer sequence of 5′-3′
SSR12 GTGCTGCTGCTCTCTTCTAT CAATGAGTAGGGTCGATGAT
SSR23 CTACTTTGATGGACACTTGCC TCTGAAGTTGATTAGTCGGTCC
SSR24 CCAGTTCCATTCTCAATCAG TATTTGTGTTCTCACGATGG
SSR39 TGAATGTGCTTCTTCGTATG TTCAAGCTCGTTCACTCTCT
SSR79 TTGTGATAGGTTGCTTCATTC CCATCTTCGTTGTCGTTG
SSR100 GATGGACAAGATATCGGAGA GCAAAGCAAAGTGAGAAGAT
SSR117 CTCCTCCTCCTTTCATCTTC TTCGTCTCCCTTCTCTGTAA

Fig.1

Amplification results of Fengyou No.10 manual hybrids and their parents with two SSR primers P1, Male parent; P2, Female parent; F1, Fengyou No.10 hybrid"

Fig.2

Partial purity identification results of hybrid Fengyou No.10 detected by two specific SSR primers P1, Male parent; P2, Female parent; 1-40, A plant of hybrid Fengyou No.10; , An abnormal plant detected by two SSR markers"

Table 2

Purity of hybrid Fengyou No.10 identified by SSR markers"

引物
Primer		 恢复系株数
Plants of restorer line		 不育系株数
Plants of sterile line		 总株数
Total plants		 恢复系比率(%)
The ratio of restorer line		 不育系比率(%)
The ratio of sterile line		 杂交种纯度(%)
Purity
SSR39 11 20 188 5.85 10.64 83.51
SSR117 8 15 188 4.26 7.98 87.77
平均值Mean 9.31 85.64
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