基于SSR标记的胡麻粗脂肪及脂肪酸组分的关联分析

doi:10.16035/j.issn.1001-7283.2022.01.006

Abstract

Abstract:

In order to understand the genetic variation of crude fat and fatty acid components in flax germplasm, the generalized linear model (GLM) was used to analyze the genetic variation of 230 flax germplasms. The results showed that the variation coefficients of crude fat and five fatty acids were 7.39%-22.67%, and the genetic diversity indexes were 2.66-2.80. The correlation coefficient between crude fat and linolenic acid was the highest (0.669). Thirty pairs of SSR primers were selected, and a total of 365 bands were amplified. The number of effective alleles ranged from 1.2168 to 1.8320, and the polymorphism of primers ranged from 0.2489 to 0.6257. The 230 flax resources could be divided into four groups when population structure K=4. There were 22 SSR loci significantly associated with five fatty acids, and four SSR loci were detected in crude fat. These results indicated that the genetic diversity of 230 flax germplasms was rich, and the contents of five fatty acids and crude fat were significantly associated with SSR markers.

Key words: Flax, Quality, Crude fat, Fatty acid components, Correlation analysis, SSR

Gao Fengyun, Siqin Bateer, Zhou Yu, Jia Xiaoyun, Su Shaofeng, Zhao Xiaoqing, Jin Xiaolei. Association Analysis of Crude Fat and Fatty Acid Components in Flax Based on SSR Markers[J].Crops, 2022, 38(1): 44-49.

Figures/Tables 8

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References 23

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引物名称Primer name	序列（5′-3′）Sequence (5′-3′)	引物名称Primer name	序列（5′-3′）Sequence (5′-3′)
Lub4	TGGAAGTCAACGAGATCGAA ACAGCAGCCTCCGTGTTTAT	Lu400	GAATGGCTCCTCGAAAGATG ATTAGACGGGGAGCTTGAGG
Lub13	CGAGGATGACAATGATGACG CAGCAGCAGCATCAGGTAAA	Lu422	GTTAATCGCCCCTGAACTGA TTGCAGTTACAACAGCAGCA
Lua37	CACAGCACAGACACAGACCA GGCGGCTTTAAGAAGTGAAA	Lu462	AATGAGCACAACAACAGCAAG AGCAGCTCTGGACTTGAGGA
Lua69	CTAAACCACACCCCCATCAC AAAGTGGGGAAATTGGGCTA	Lu465	CAAGACTTGTAGGGCGGAAC CGTCGGCCTATGAGAAGAAC
Lua83B	CCCTCATTTTTCTCCTTCCA CAGGCGTTACAGTTTCCCATA	Lu511	CATTGACCTCCCATTTCACC TCAAGGAAGGCTCGTTGTTC
Lua125a	GCCTTTGGAGGGCTTAACTT ACAATCCCAACATTCCCAAA	Lu554	GGCCAAGGATATAGCACGAA TTGGACCTTAAGCCCAGATG
Lu146	AACCTGAACCAGACGAGCAT AGGTGGATCCAGCAAGCTAA	Lu598	TAGAGGCCAGCTAGCAGCA AAAAGCTTCCCTTTGGTGGT
Lu176	TCCATCCTCTGCATTTGTGA AAGACGAGTGCCCATTCCTA	Lu661	AAGACAACAACCTGGGGAAA GATTCAGCAGCCGAGAGTG
Lu203a	CCTTTTCACGCAGAGCTACC GCTTCCGTAATCCTCTTCCA	Lu747	CGGCTGAGGATTACTTGTCG TAAACTCCACTTCCCCCAAC
Lu263	GCCGAAAGTTGAAGCATAGG TGTTGCTTGTTGGCAAACTG	Lu765B	CCTCATTCCGCTCAGCAA CGAAAATGGGGAAGATGATG
Lu266	ACGACACCGGATTTATCTGC ACGTGTCCTCCACATGCTCT	Lu771	ATACTCCTCCGACGCTGATG AACCTCGAAACGAATGATGC
Lu273	CGATGATCACTGGACGGATA CATAGCTTCAAAGGCAGCAC	Lu785	CGAGGCATCATATTTTCTCTTG ATCAGCAATCAATCGCATCA
Lu291	GGAAATTCCAAGTTCCCAGT AGTTTCGCTATTCCGTCTGC	Lu787	AAGACCACCACAAGGGACAG TGAACCATAGCGATCATCACA
Lu316	TCCTCGGAAGAAGAAGACGA GAGAGGAATCATGGCGGATA	Lu840	ATTCCTTTTTGAGGGCGAGT ACAGCTGGAACTGGAGAGGA
Lu330	TCTTGTACATTGCGGCACTC GCACCAGATGAGGAAGAGGA	Lu849	CGACACAGCATTCAATGACC CAGACCTTGGAGCTTTGGAG

品质性状 Quality characteristic	最小值 Min. (%)	最大值 Max. (%)	均值 Mean (%)	标准差 Standard deviation	变异系数 CV (%)	遗传多样性指数 Genetic diversity index
粗脂肪含量Crude fat content	35.51	43.21	39.01	2.88	7.39	2.80
棕榈酸含量Palmatic acid content	3.00	6.27	4.45	0.75	16.28	2.74
硬脂酸含量Stearic acid content	3.65	13.96	7.82	1.77	22.67	2.66
油酸含量Oleic acid content	6.52	32.68	20.26	3.66	18.08	2.72
亚油酸含量Linoleic acid content	11.30	20.20	16.24	1.91	11.74	2.78
亚麻酸含量Linolenic acid content	39.35	65.29	49.67	4.54	9.14	2.80

品质性状 Quality characteristic	棕榈酸含量 Palmatic acid content	硬脂酸含量 Stearic acid content	油酸含量 Oleic acid content	亚油酸含量 Linoleic acid content	亚麻酸含量 Linolenic acid content	粗脂肪含量 Crude fat content
棕榈酸含量Palmatic acid content	1
硬脂酸含量Stearic acid content	-0.429^**	1
油酸含量Oleic acid content	0.360^**	0.054	1
亚油酸含量Linoleic acid content	0.147^*	0.394^**	-0.03	1
亚麻酸含量Linolenic acid content	0.308^**	-0.031	-0.191^**	-0.395^**	1
粗脂肪含量Crude fat content	0.483^**	0.106	0.388^*	0.273^**	0.669^**	1

引物名称 Primer name	多态性位点数 Number of polymorphic loci	有效等位基因数 Number of effective alleles	引物PIC Primer PIC	引物名称 Primer name	多态性位点数 Number of polymorphic loci	有效等位基因数 Number of effective alleles	引物PIC Primer PIC
Lu4	7	1.4053	0.4253	Lu400	13	1.3216	0.3423
Lu13	11	1.4162	0.4085	Lu422	9	1.7526	0.6117
Lu37	11	1.8230	0.6257	Lu462	19	1.4617	0.4422
Lu69	8	1.1991	0.2489	Lu465	13	1.5082	0.4545
Lu83B	13	1.6207	0.5440	Lu511	10	1.3910	0.3927
Lu125a	12	1.2168	0.2765	Lu554	5	1.5741	0.4998
Lu146	15	1.4852	0.4765	Lu598	15	1.4779	0.4105
Lu176	9	1.3284	0.3788	Lu661	11	1.2276	0.2896
Lu203a	13	1.5499	0.5059	Lu747	14	1.4020	0.4183
Lu263	10	1.3633	0.3841	Lu765B	14	1.4558	0.4320
Lu266	6	1.4834	0.4819	Lu771	10	1.3083	0.3340
Lu273	12	1.2813	0.3548	Lu785	14	1.3927	0.3997
Lu291	18	1.3264	0.3638	Lu787	14	1.6178	0.5364
Lu316	17	1.3894	0.3878	Lu840	16	1.4884	0.4625
Lu330	10	1.5515	0.4904	Lu849	15	1.4865	0.4571

品质性状 Quality characteristic	SSR位点 SSR loci	P值 P-value	表型变异解释率 Phenotypic variation (%)		品质性状 Quality characteristic	SSR位点 SSR loci	P值 P-value	表型变异解释率 Phenotypic variation (%)
粗脂肪含量 Crude fat content	S116	6.69	3.03			硬脂酸含量 Stearic acid content	S36	5.64	3.01
粗脂肪含量 Crude fat content	S121	7.89	2.91	S241		硬脂酸含量 Stearic acid content	6.58	2.91
	S230	9.39	2.79			S354	8.15	2.76
	S158	9.97	2.74			S83	8.41	2.73
棕榈酸含量 Palmatic acid content	S190	6.89	2.72			S216	9.33	2.66
棕榈酸含量 Palmatic acid content	S110	7.52	2.66		亚油酸含量 Linoleic acid content	S99	6.13	3.26
	S51	7.71	2.61		亚油酸含量 Linoleic acid content	S159	6.31	3.24
	S254	8.13	2.52			S167	6.49	3.22
	S60	9.39	2.45			S116	6.75	3.19
油酸含量 Oleic acid content	S342	5.20	3.44			S28	6.91	3.17
油酸含量 Oleic acid content	S115	7.30	3.17			S160	7.67	3.09
	S353	8.64	3.04			S347	8.51	3.01
亚麻酸含量 Linolenic acid content	S347	5.28	3.35			S304	9.84	2.90

Association Analysis of Crude Fat and Fatty Acid Components in Flax Based on SSR Markers

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