Crops ›› 2022, Vol. 38 ›› Issue (2): 153-157.doi: 10.16035/j.issn.1001-7283.2022.02.021

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Effects of Light and Temperature on Photoautotrophic Rooting for in Vitro Sugarcane Plantlets

Liu Limin(), Liu Hongjian, Li Aomei, He Weizhong()   

  1. Sugarcane Research Institute, Guangxi Academy of Sciences/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Nanning 530007, Guangxi, China
  • Received:2022-02-09 Online:2022-04-15 Published:2022-04-24
  • Contact: He Weizhong E-mail:64225211@qq.com;Csn899@aliyun.com

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Abstract:

To improve the rate of photoautotrophic rooting for in vitro sugarcane plantlets under artificial planting condition, treatments with different light and temperature compensation and light quality were carried out. The effects of these factors on rooting and growth were analyzed through characters of rooting rate, survival rate, seedling height, root length and root number per plant for rootless seedlings. The results showed that the rooting rate and survival rate for photosynthetic autotrophic rooting of sugarcane in vitro plantlets decreased in winter due to the low temperature and low light compared with the summer. In winter light supplementation improved the photosynthetic autotrophic rooting length and seedling growth for in vitro plantlets, but there was no significant effect on the rooting rate and survival rate. Compared with the natural light treatment, pure irradiation of red light and blue light treatments were not conducive to the rooting and growth of sugarcane in vitro plantlets, while natural light supplemented by blue light and red light could promote the seedling height and root elongation for in vitro plantlets, but there was no significant effect on the rooting rate and survival rate of in vitro plantlets.

Key words: Sugarcane, In vitro plantlets, Light and temperature, Photosynthetic autotrophic rooting

Table 1

Daily temperature and illumination intensity in different seasons"

移栽处理
Transplanting treatment		 温度Temperature (°C) 光照强度Illumination intensity(lx)
平均值Average 最大值/最小值Max./Min. 平均值Average 最大值Max.
夏季移栽Transplanting in summer 31.1 47.2/25.5 4 168.1 31 354.9
冬季移栽Transplanting in winter 16.9 39.5/10.2 1 880.0 22 677.0

Table 2

Effects of different transplanting seasons on photosynthetic autotrophic rooting of sugarcane in vitro plantlets %"

移栽处理
Transplanting treatment		 GT44 GT49
生根率Rooting rate 存活率Survival rate 生根率Rooting rate 存活率Survival rate
夏季移栽Transplanting in summer 100.0 98.2 100.0 85.0
冬季移栽Transplanting in winter 82.2 95.1 50.1 63.3

Table 3

Temperature and illumination intensity of supplementary light greenhouse and natural light greenhouse"

处理
Treatment		 温度Temperature (°C) 光照强度Illumination intensity (lx) 补光时数
Hours of supplementary lighting (h/d)
平均值Average 最大值/最小值Max./Min. 平均值Average 最大值Max.
光补偿
Supplementary lighting		 18.3 40.9/10.2 2 019.0 24 617.0 12
自然光（对照）
Natural light (CK)		 17.9 41.0/9.8 1 879.9 22 677.6 0

Fig.1

Effects of light and temperature compensation on photosynthetic autotrophic rooting of different sugarcane cultivars in vitro plantlets"

Table 4

Effects of light supplementation on rooting and growth of sugarcane in vitro plantlets"

品种/处理
Variety/treatment		 苗高
Seedling height (cm)		 根长
Root length (cm)		 单株根数
Root number per plant		 生根率
Rooting rate (%)		 存活率
Survival rate (%)
GT44 23.7aA 6.1aA 2.2aA 77.5aA 95.0aA
GT43 14.4bB 3.6bB 1.5bA 76.3aA 45.4cC
GT49 14.6bB 3.0bB 0.6cB 51.3bA 59.0bB
光补偿Supplementary lighting 19.7** 5.8** 1.9** 75.2 66.8
自然光（对照）Natural light (CK) 15.4 2.7 1.0 61.5 63.0

Table 5

Temperature and illumination intensity of different light quality treatments"

处理
Treatment		 温度Temperature (°C) 光照强度Illumination intensity (lx) 补光时数
Hours of supplementary lighting (h/d)
平均值Average 最大值/最小值Max./Min. 平均值Average 最大值Max.
自然光+红光
Natural light + red light		 32.2 50.9/25.9 2 308.4 31 109.1 12
自然光+蓝光
Natural light + blue light		 32.4 47.8/25.7 2 514.4 27 105.3 12
红光Red light 31.0 44.1/25.7 95.0 3 763.2 12
蓝光Blue light 31.0 44.3/25.9 71.0 1 071.7 12
自然光（对照）
Natural light (CK)		 32.3 48.3/25.8 1 963.9 16 640.0 –

Table 6

Effects of light on the growth and root growth of GT44 in vitro plantlets"

处理
Treatment		 苗高
Seedling height (cm)		 根长
Root length (cm)		 单株根数
Root number per plant		 生根率
Rooting rate (%)		 存活率
Survival rate (%)
自然光+红光Natural light + red light 33.8aAB 8.8aA 10.0bB 100.0a 94.0aA
自然光+蓝光Natural light + blue light 35.2aA 7.8aA 11.0bA 100.0a 95.0aA
红光Red light – – – – 0.0bB
蓝光Blue light – – – – 0.0bB
自然光（对照）Natural light（CK） 30.6bB 7.7aA 12.0aA 100.0a 94.0aA
[1] 游建华, 曾慧, 李松, 等. 甘蔗脱毒健康种苗田间比较试验. 中国糖料, 2005(2):12-15.
[2] 沈万宽, 郑学文, 陈仲华, 等. 甘蔗健康种苗田间评价试验结果分析. 西南农业学报, 2009, 22(4):913-915.
[3] 杨本鹏, 张树珍, 蔡文伟, 等. 甘蔗健康种苗田间栽培主要农艺性状比较. 热带作物学报, 2010, 31(2):171-175.
[4] 何为中, 刘红坚, 李松, 等. 甘蔗试管苗瓶外生根技术的研究. 安徽农业科学, 2012, 40(29):14191-14194.
[5] 何为中, 范业赓, 刘丽敏, 等. 甘蔗试管苗光合自养生根技术研究. 广西植物, 2018, 38(10):1298-1309.
[6] 刘红坚, 范业赓, 何为中, 等. 甘蔗试管苗瓶内生根与瓶外生根培养方法比较研究. 广东农业科学, 2013, 40(18):13-15.
[7] 刘丽敏, 何为中, 刘红坚, 等. 甘蔗无根试管苗光合自养生根过程中叶片生理生化特征的变化. 热带作物学报, 2018, 39(5):867-872.
[8] 潘瑞炽, 王小菁, 李娘辉. 植物生理学. 北京: 高等教育出版社, 2012.
[9] 张露荷, 赵通, 黄华梨, 等. 不同光温处理对“黄金芽”叶片生理的影响. 林业科技通讯, 2020(5):14-19.
[10] 蔡淑芳, 吴宝意, 廖水兰, 等. 基于光温效应的温室小白菜生理指标动态模拟. 南方农业学报, 2020, 51(9):2191-2198.
[11] 陆佳岚, 马成, 陶明煊, 等. 不同光温条件对水稻9311产量及品质的影响. 江苏农业学报, 2020, 36(3):535-543.
[12] 汪淼, 方锋学, 何为中, 等. 外源激素处理对甘蔗试管苗瓶外生根的影响. 安徽农业科学, 2012, 40(16):8833-8835.
[13] 曹加杰, 陆俊安, 阮宏华. 水下光照补偿对沉水植物苦草生长及主要生理指标的影响. 南京林业大学学报(自然科学版), 2014, 38(5):102-106.
[14] 孙庆丽, 陈志, 徐刚, 等. 不同光质对水稻幼苗生长的影响. 浙江农业学报, 2010, 22(3):321-325.
[15] 闫萌萌, 王铭伦, 王洪波, 等. 光质对花生幼苗根系生长与根系活力的影响. 农学学报, 2013, 3(8):17-20.
[16] Liu X Y, Jiao X L, Chang T T, et al. Photosynthesis and leaf development of cherry tomato seedlings under different LED-based blue and red photon flux ratios. Photosynthetica, 2018, 56(4):1212-1217.
doi: 10.1007/s11099-018-0814-8
[17] 董飞, 王传增, 张现征, 等. 不同光质对樱桃番茄幼苗生理与光合特性的影响. 植物生理学报, 2017, 53(7):1208-1214.
[18] 杨俊伟, 鲍恩财, 张珂嘉, 等. 不同红蓝光比例对番茄幼苗叶片结构及光合特性的影响. 西北农业学报, 2018, 27(5):716-726.
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