Crops ›› 2020, Vol. 36 ›› Issue (2): 194-199.doi: 10.16035/j.issn.1001-7283.2020.02.030

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The Difference of Single Bud Seedling of the Third Generation of Sugarcane Virus-Free Plantlets with Different Seedcane Sizes and Transplanting Effect

Fan Yegeng1,Yan Haifeng1,Chen Rongfa1,Qiu Lihang1,Zhou Huiwen1,Huang Xing1,Weng Mengling1,Wu Jianming1(),Li Yangrui1,Wei Shengman2   

  1. 1 Sugarcane Research Institute/Guangxi Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement of Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Guangxi Sugarcane Genetic Improvement, Nanning 530007, Guangxi, China
    2 Lüjiang Professional Breeding Cooperative in Zhoulu Town, Mashan County, Nanning 530603, Guangxi, China
  • Received:2019-07-30 Revised:2020-03-06 Online:2020-04-15 Published:2020-04-13
  • Contact: Jianming Wu E-mail:wujianming2004@126.com

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Abstract:

To change the existing mode of sugarcane cultivation and realizing the change from ‘sugarcane stem planting’ to ‘sugarcane seedling planting’, so it can eliminate the purpose of farmers' self-reservation of stem and to increase the sugarcane yield and sucrose content. The third generation seedcane of Guitang 42 derived from regenerated sugarcane virus-free plantlets as material, four treatments of seedcane including complete single bud, 1/2 single bud, 1/4 single bud and conventional seedcane (control) were adopted to study the effects of seedling breeding and transplanting of different sizes of bud. The plant height, seedling diameter, number of leaves, root fresh weight and root dry weight of the treatments with single bud were significantly higher than that of the treatments with 1/2 single bud and 1/4 single bud. The number of leaves, root fresh weight and root dry weight of the treatment with 1/2 single bud were also significantly higher than that of the treatment with 1/4 single bud, but the difference of plant height and seedling diameter between them wasn’t significant. The treatments (complete single bud, 1/2 single bud, 1/4 single bud) transplanting survival rate, effective stem number were superior to the control, and yield and sucrose content is higher than the control by 18.13% and 1.10 percentage points, especially the yield and sucrose content of the treatment with complete single bud. Among the four treatments, the agronomic characters, yield of raw sugarcane and sucrose content of the treatment with complete bud were the best.

Key words: Sugarcane, Virus-free plantlet, Single bud, Transplant, Yield, Sucrose content

Table 1

The changes of sugar production, sugarcane yield and sucrose content in Guangxi from 2001 to 2018"

榨季
The milling period		 总产糖量(万t)
Total sugar production (×104 t)		 原料蔗平均单产(t/hm2)
Average yield of raw sugarcane		 平均蔗糖分(%)
Average sucrose content		 榨季
The milling period		 总产糖量(万t)
Total sugar production (×104 t)		 原料蔗平均单产(t/hm2)
Average yield of raw sugarcane		 平均蔗糖分(%)
Average sucrose content
2001-2002 456.00 63.15 13.68 2010-2011 675.20 56.25 13.93
2002-2003 563.00 67.95 13.51 2011-2012 694.30 55.50 13.85
2003-2004 588.00 68.25 14.76 2012-2013 795.00 63.90 13.58
2004-2005 531.60 68.70 14.78 2013-2014 857.56 70.05 13.85
2005-2006 537.70 69.15 14.27 2014-2015 639.24 60.75 13.84
2006-2007 709.00 68.70 14.59 2015-2016 510.42 62.40 13.21
2007-2008 941.00 79.50 14.42 2016-2017 529.50 59.55 13.88
2008-2009 763.80 60.45 14.30 2017-2018 602.50 67.05 13.53
2009-2010 711.80 56.70 14.63

Fig.1

The sugarcane seedling process of single buds with different bud sizes S1, S1/2 and S1/4 are sugarcane seedling bred from complete single bud, 1/2 single and 1/4 single bud, respectively"

Fig.2

Growth analysis of single buds with different sizes Different uppercase letters and lowercase letters indicate extremely significant difference (P < 0.01) and significant difference (P < 0.05), respectively"

Table 2

Comparison of sugarcane agronomic characters in different treatments"

处理
Treatment		 成活率(%)
Survival rate		 分蘖率(%)
Tillering rate		 株高(cm)
Plant height		 茎径(mm)
Stem diameter		 有效茎数(条/hm2)
Effective stem number (Tiao/hm2)
完整单芽Complete single bud 99.39aA 116.00aA 226.82aA 27.00aA 56 219aA
1/2单芽1/2 single bud 98.55aA 98.66aA 209.29bA 27.24aA 55 266abA
1/4单芽1/4 single bud 96.17aA 47.34bB 200.31bA 26.24aA 55 028abA
常规种茎Conventional seedcane 84.10bB 54.66bB 210.27bA 26.72aA 50 740bB

Table 3

Yield comparison of raw sugarcane under different treatments"

处理
Treatment		 小区产量Plot yield (kg) 产量
Yield (t/hm2)		 提高比率(%)
Increase rate
Ⅰ Ⅱ Ⅲ 平均Average
完整单芽Complete single bud 335.61 353.69 324.08 337.80aA 112.66aA 18.13
1/2单芽1/2 single bud 298.55 308.49 306.91 304.65bAB 101.60bAB 6.53
1/4单芽1/4 single bud 307.81 299.90 297.42 301.71bB 100.62bB 5.50
常规种茎Conventional seedcane 283.63 276.62 297.64 285.96bB 95.37bB 0.00

Table 4

Sucrose content comparison of different treatments"

处理
Treatment		 蔗糖分Sucrose content (%) 平均蔗糖分(%)
Average sucrose content		 提高比率(%)
Increase rate
Ⅰ Ⅱ Ⅲ
完整单芽Complete single bud 14.00 14.96 14.46 14.47aA 8.23
1/2单芽1/2 single bud 13.40 14.56 14.88 14.28abA 6.81
1/4单芽1/4 single bud 14.00 13.56 13.28 13.61bcA 1.80
常规种茎Conventional seedcane 19.52 14.04 13.36 13.37cA 0
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