Crops ›› 2024, Vol. 40 ›› Issue (4): 263-268.doi: 10.16035/j.issn.1001-7283.2024.04.034

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Effects of Humic Acid Suspension Agent on Seed Germination and Seedling Growth of Wheat under NaCl Stress

Zhang Ziyi(), Wang Xuehu, Yuan Ying, Shen Zhifeng   

  1. The Research and Development Department of Hebei Monband Biotech Co., Ltd., Xingtai 054300, Hebei, China
  • Received:2023-06-16 Revised:2023-09-15 Online:2024-08-15 Published:2024-08-14

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Abstract:

Humic acid with different molecular weights have different functional characteristics. This experiment aimed to investigate the effects of different molecular weight humic acids on the growth of wheat seedlings under NaCl stress, which was beneficial to promoting the rational development and utilization of humic acid resources. The double-layer filter paper culture method was used in the experiment, and five treatments were set as follows: clear water (T1), 200 mmol/L NaCl solution (T2), T2+humic acid suspension I (adding high molecular weight, T3), T2+humic acid suspension II (adding medium molecular weight, T4), T2+humic acid suspension III (adding small molecular weight, T5). The results showed compared with T2 treatment, the addition of humic acid could significantly reduce the relative conductivity of wheat roots, and increase the germination percentage, root length, plant height, soluble sugar content, total chlorophyll content, and the activities of peroxidase, superoxide dismutase, and catalase of wheat. The above indicators increased by 17.81%, 68.46%, 90.63%, 21.37%, 19.88%, 61.07%, 14.59%, 30.70%, respectively in T3 treatment; in T4 treatment, increased by 20.12%, 101.80%, 80.21%, 33.42%, 23.39%, 62.19%, 39.43% and 46.73%, respectively; In T5 treatment, increased by 21.26%, 166.67%, 54.17%, 35.73%, 24.56%, 63.43%, 43.60%, 48.22%, respectively. Comprehensive analysis showed that different molecular weights of humic acid could improved the germination percentage and the salt resistance of seedlings. Among them, high molecular weight humic acid had a significant effect on promoting the growth of wheat leaves, while small molecular weight humic acid had played an important role in promoting the growth of wheat roots, improving the activity of antioxidant enzymes in plants and enhancing the stability of cell membranes.

Key words: Wheat, Humic acid with different molecular weights, NaCl stress, Seed germination, Seedling growth characteristics, Antioxidant enzyme

Table 1

Effects of seed soaking with humic acid suspension agent with different molecular weights on wheat seed germination under NaCl stress"

处理
Treatment		 发芽势
Germination
potential (%)		 发芽率
Germination
percentage (%)		 发芽指数
Germination
index		 活力指数
Vitality
index
T1 76.67±2.33a 90.00±3.00a 38.67±0.70a 174.01±3.18a
T2 34.33±4.06c 58.00±4.04c 17.12±1.39c 19.08±1.54c
T3 44.67±3.18b 68.33±1.43b 21.88±0.74b 27.83±0.95c
T4 45.21±1.53b 69.67±1.45b 22.15±1.41b 31.47±2.64b
T5 45.34±2.08b 70.33±1.86b 22.49±0.99b 47.14±2.14b

Fig.1

Effects of seed soaking with humic acid suspension agent with different molecular weights on root length and plant height of wheat under NaCl stress Different lowercase letters indicate significant differences among different treatments (P < 0.05), the same below."

Fig.2

Effects of seed soaking with humic acid suspension agent with different molecular weights on contents of chlorophyll and soluble sugar of wheat leaves under NaCl stress"

Fig.3

Effects of seed soaking with humic acid suspension agent with different molecular weights on relative conductivity of wheat leaves and roots under NaCl stress"

Fig.4

Effects of seed soaking with humic acid suspension agent with different molecular weight on antioxidant enzyme activity of wheat leaves under NaCl stress"

Table 2

Correlation analysis of wheat growth, electrical conductivity and antioxidant enzyme activity under different molecular weight humic acid suspensions agent"

指标
Index		 发芽势
Germination
potential		 发芽率
Germination
percentage		 根长
Root
length		 株高
Plant
height		 总叶绿素
含量
Total
chlorophyll
content		 可溶性糖
含量
Soluble
sugar
content		 叶片相对
电导率
Relative
conductivity
of leaf		 根相对
电导率
Relative
conductivity
of root		 SOD PDD CAT
发芽势Germination potential 1.000
发芽率Germination percentage 0.986** 1.000
根长Root length 0.944* 0.931* 1.000
株高Plant height 0.969** 0.919* 0.885* 1.000
总叶绿素含量Total chlorophyll content 0.651 0.0748 0.747 0.447 1.000
可溶性糖含量Soluble sugar content -0.989** -0.955* -0.914* -0.994** -0.537 1.000
叶电导率Relative conductivity of leaf -0.970** -0.996** -0.925* -0.883* -0.798 0.927* 1.000
根电导率Relative conductivity of root -0.974** -0.995** -0.909* -0.904* -0.742 0.946* 0.988** 1.000
SOD -0.642 -0.552 -0.469 -0.785 0.103 0.743 0.477 0.577 1.000
PDD -0.541 -0.413 -0.374 -0.725 0.271 0.646 0.344 0.387 0.852 1.000
CAT -0.670 -0.559 -0.504 -0.829 0.126 0.769 0.488 0.552 0.949* 0.967** 1.000
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