Crops ›› 2025, Vol. 41 ›› Issue (4): 126-134.doi: 10.16035/j.issn.1001-7283.2025.04.016

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Preliminary Study on the Waterlogging Tolerance Mechanisms in Two Cotton Varieties at Seedling Stage during Waterlogging Stress and Recovery Period

Liu Xuanxuan1(), Guo Ruishi2, Dong Mengmeng1, Zhu Keying1, Zhu Xiaopin1, Wang Li1, Wang Ning2()   

  1. 1College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China
    2Institute of Cotton Research, Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Anyang 455000, Henan, China
  • Received:2025-02-28 Revised:2025-04-03 Online:2025-08-15 Published:2025-08-12

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Abstract:

In order to investigate physiological response mechanisms of waterlogging tolerance in cotton seedlings, the waterlogging tolerant variety Zhongmian 9001 (ZM9001) and the waterlogging sensitive variety Zhong 4847 (Z4847) were used as experimental materials to compare the dynamic physiological responses of the two varieties to waterlogging stress and post-waterlogging recovery. The results showed that under waterlogging for 4 d (recovery 0 h) and post-waterlogging recovery stages (24 and 72 h), ZM9001 exhibited significantly higher net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr), but lower J-point and I-point values of the chlorophyll fluorescence induction kinetic curve (OJIP curve) compared to Z4847. The abscisic acid (ABA) content was significantly lower in ZM9001 than that in Z4847. At four days of waterlogging treatment, there was no significant difference in the contents of superoxide anion (O2-. ), malondialdehyde (MDA), and total antioxidant capacities between the two varieties. At 72 hours of waterlogging recovery, the content of O2-. and MDA of ZM9001 were significantly lower than those of Z4847, and its antioxidant capacity was significantly higher than that of Z4847. The hydrogen peroxide (H2O2) content was higher in ZM9001 than that in Z4847 under waterlogging for 4 d and during 72 h of recovery. At four days after waterlogging and the recovery stages, the catalase (CAT) activity in ZM9001 was significantly higher than that in Z4847. At 72 hours of post-waterlogging recovery, ZM9001 showed higher superoxide dismutase (SOD) activity and higher flavonoid content in different organs than Z4847. In summary, compared to Z4847, ZM9001 maintained lower ABA content and higher Gs and Tr during waterlogging and recovery periods, while efficiently scavenging reactive oxygen species (ROS) by enhancing antioxidant system activity during recovery stage. These enables ZM9001 to sustain superior photosynthetic capacity and waterlogging tolerance.

Key words: Cotton, Waterlogging stress, Waterlogging recovery period, Photosynthesis, Reactive oxygen, Antioxidant enzyme

Fig.1

Pn and gas exchange parameters of cotton seedling leaves during waterlogging stress and the recovery period Different lowercase letters indicate the significant differences among treatments (P < 0.05). The same below."

Fig.2

Kinetics curve of chlorophyll fluorescence induction in cotton seedlings during waterlogging stress and the recovery period"

Fig.3

Chlorophyll fluorescence radargram of cotton seedlings during waterlogging stress and the recovery period"

Fig.4

O2-. , H2O2 and MDA content of cotton seedlings during waterlogging stress and the recovery period"

Fig.5

Total antioxidant capacity and antioxidant enzyme activity of cotton seedlings during waterlogging stress and the recovery period"

Fig.6

Flavonoid and ABA contents of cotton seedlings during waterlogging stress and the recovery period"

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