Crops ›› 2025, Vol. 41 ›› Issue (4): 75-79.doi: 10.16035/j.issn.1001-7283.2025.04.009

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Composition Analysis of High Molecular Weight Glutenin Subunits in 121 Wheat Varieties (Lines)

Dou Yang1(), Chen Junyi1, Ren Shumin1, Kang Guozhang2, Wang Liming1()   

  1. 1College of Agriculture, Henan University of Science and Technology, Luoyang 471023, Henan, China
    2Henan Agricultural University / Co-Construction State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, Henan, China
  • Received:2024-05-16 Revised:2024-07-04 Online:2025-08-15 Published:2025-08-12

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Abstract:

The high molecular weight glutenin subunits (HMW-GS) of 121 self-selected high generation wheat varieties (lines) were identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), and their subunit types and combinations were analyzed. The results showed that there were nine subunit types in 121 wheat varieties (lines). Among them, there were two types of Glu-A1 sites, including 1 and N. There were four kinds of Glu-B1 sites, including 7, 7+8, 7+9 and 13+16. There were three kinds of Glu-D1 sites, including 5+10, 4+12 and 2+12. There were ten subunit combinations in 121 wheat varieties (lines), among which 1/7+ 8/5+10 subunit combinations were the most with a frequency of 52.90%, followed by 1/7+9/5+10, 1/7+8/2+12, 1/7+9/2+12, and their frequency was 23.14%, 9.10%, 5.79%, respectively. The frequency of other subunit combinations was less than 5%. The quality scores of all varieties (lines) ranged from 5-10. The results showed that there were abundant polymorphisms of HMW-GS in all the 121 new wheat varieties (lines). The high-quality subunit 5+10 had the highest frequency, rare subunit 13+16 was found, and no high-quality subunits 2*, 14+15 and 17+18 were found.

Key words: Wheat, Varieties (lines), High molecular weight glutenin subunits, Subunit composition

Table 1

HMW-GS composition of 121 wheat varieties (lines)"

编号
Code		 HMW-GS 品种（系）编号
Variety (line) code		 品种（系）个数
Variety (line) number
Glu-A1 Glu-B1 Glu-D1
1 N 7+8 2+12 Q1、Q31、Q102 3
2 N 7+8 5+10 Q92、Q106 2
3 N 13+16 2+12 Q30 1
4 N 7+8 4+12 Q59、Q82 2
5
 1
 7+8
 5+10
 Q2-Q19、Q21-Q26、Q33、Q36-Q58、Q70、Q78、Q83、Q86、Q87、Q90、Q94-Q98、Q103-Q105、Q115、Q121 64
6 1 7+8 2+12 Q20、Q32、Q34、Q35、Q88、Q89、Q91、Q93、Q101、Q118、Q119 11
7 1 7+9 2+12 Q68、Q69、Q73-Q76、Q84 7
8
 1
 7+9
 5+10
 Q60-Q67、Q71、Q72、Q77、Q79-Q81、Q85、Q99、Q100、Q107-Q114、Q116、Q117、Q120 28
9 1 7 4+12 Q27 1
10 1 13+16 2+12 Q28、Q29 2

Fig.1

SDS-PAGE analysis of HMW-GS in some wheat materials"

Fig.2

HMW-GS occurrence frequencies of 121 varieties (lines) in each gene locus"

Table 2

Subunit combination types, frequencies and quality scores of 121 varieties (lines)"

编号Code 亚基组合Subunit combination 品种（系）数Number of varieties (lines) 频率Frequency (%) 品质得分Quality score
1 N/7+8/2+12 3 2.48 6
2 N/7+8/5+10 2 1.65 8
3 N/13+16/2+12 1 0.83 6
4 N/7+8/4+12 2 1.65 5
5 1/7+8/5+10 64 52.90 10
6 1/7+8/2+12 11 9.10 8
7 1/7+9/2+12 7 5.79 7
8 1/7+9/5+10 28 23.14 9
9 1/7/4+12 1 0.83 5
10 1/13+16/2+12 2 1.65 8
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