Crops ›› 2026, Vol. 42 ›› Issue (1): 20-25.doi: 10.16035/j.issn.1001-7283.2026.01.004

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Identification and Evaluation of Resistance to Acanthoscelides obtectus in Common Bean Germplasm Resources

Wang Qinfang1(), Tang Yongsheng1(), Jiang Yanhua1, Zhang Juxiang1, Yu Haitian2, Zhu Yufen1, Zhu Zhendong3   

  1. 1Qujing Academy of Agricultural Science, Qujing 655000, Yunnan, China
    2Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China
    3Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2024-08-19 Revised:2024-10-19 Online:2026-02-15 Published:2026-02-10

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Abstract:

The resistance of 473 common bean resources to Acanthoscelides obtectus was identified by artificial inoculation method. The results showed that 49.1% of the resources showed varying degrees of resistance, mcluding 15 highly resistant resources, 60 resistant resources and 157 moderately resistant resources. In the highly resistant resources, F1432, F0645, F0675, F0004351 and F0004406 did not show any infected symptoms, manifesting as immunity. Research found that A.obtectus has a certain phototaxis. No significant differences in resistance were found among resources with different grain color, grain size, and seed coat luster. The screening and identification of A.obtectus resistant resources is beneficial for providing excellent resistance resources for subsequent resistance breeding.

Key words: Common bean, Acanthoscelides obtectus, Resistance identification, Infestation rate

Table 1

Resources of common bean"

编号
Number		 品种（系）
Variety (line)		 编号
Number		 品种（系）
Variety (line)		 编号
Number		 品种（系）
Variety (line)		 编号
Number		 品种（系）
Variety (line)		 编号
Number		 品种（系）
Variety (line)		 编号
Number		 品种（系）
Variety (line)
1 A1800 50 A1865-1 99 A1940 148 A2001 197 A2081 246 F0698
2 A1806-1 51 A1866 100 A1941 149 A2002-2 198 A2084 247 F0700
3 A1809-1 52 A1867 101 A1942 150 A2003 199 A2088-2 248 F1118
4 A1810 53 A1868-1 102 A1943 151 A2004 200 A2100 249 F1124
5 A1811-1 54 A1869 103 A1944 152 A2005 201 A2101 250 F0099
6 A1815 55 A1870 104 A1945 153 A2006 202 A2103 251 F0096
7 A1816 56 A1871 105 A1946 154 A2007 203 A2105 252 F0079
8 A1818 57 A1872 106 A1947 155 A2008 204 A2106 253 F0077
9 A1819 58 A1873-1 107 A1948 156 A2009 205 A2107 254 F0072
10 A1820 59 A1874 108 A1949 157 A2010 206 A2108 255 F1159
11 A1823 60 A1875 109 A1950 158 A2011 207 A2111 256 F1192
12 A1824 61 A1876 110 A1951 159 A2012 208 A2112 257 F1211
13 A1825-1 62 A1878 111 A1952 160 A2014 209 A2113 258 F1214
14 A1826 63 A1879 112 A1954 161 A2015 210 A2117 259 F1222
15 A1827 64 A1880 113 A1955 162 A2023-2 211 A2122-2 260 F1224
16 A1828 65 A1881 114 A1956 163 A2024 212 A2125 261 F1227
17 A1829 66 A1882 115 A1957 164 A2025 213 F0004837 262 F1246
18 A1832 67 A1883 116 A1958-1 165 A2026 214 F0004404 263 F1257
19 A1833 68 A1884 117 A1961 166 A2031 215 F0004356 264 F1477
20 A1834 69 A1885 118 A1962-2 167 A2032 216 F0004845×RF 265 F1466
21 A1835 70 A1886 119 A1964-1 168 A2033 217 F0004341×RF 266 F1474
22 A1836 71 A1891 120 A1965 169 A2034 218 F0004339×RF 267 F1473
23 A1837 72 A1893 121 A1966-1 170 A2035 219 F0004381×RF 268 F1442
24 A1838 73 A1894 122 A1967-1 171 A2036 220 F0004357×RF 269 F1163
25 A1839 74 A1895-1 123 A1968 172 A2037 221 F0004413 270 F1164
26 A1840 75 A1905 124 A1969 173 A2038 222 F0004910×RF 271 F1165
27 A1841 76 A1906 125 A1970 174 A2039 223 品芸2号 272 F1166
28 A1842 77 A1907 126 A1971 175 A2041 224 龙芸豆3号 273 F1195
29 A1843 78 A1909-1 127 A1972-1 176 A2042 225 龙芸豆5号 274 F1140
30 A1844 79 A1910-1 128 A1973 177 A2044 226 龙22-0579×RF 275 F1142
31 A1846 80 A1911 129 A1974 178 A2046 227 NR 276 F1154
32 A1847 81 A1912 130 A1976 179 A2047 228 NV×RF 277 F1158
33 A1848 82 A1917 131 A1977-1 180 A2048 229 F0004349×RF 278 F1198
34 A1849 83 A1918 132 A1978 181 A2049 230 F0004396×RF 279 F0665
35 A1850 84 A1919 133 A1979 182 A2053 231 F0004322 280 F0673
36 A1851 85 A1920 134 A1980 183 A2054 232 F0004923 281 F0674
37 A1852 86 A1921 135 A1981-1 184 A2055 233 F0004425×RF 282 F0677
38 A1853 87 A1922 136 A1985 185 A2058 234 F0004890×RF 283 F0678
39 A1854 88 A1926 137 A1986 186 A2062 235 F0004926×RF 284 F0151
40 A1855 89 A1927 138 A1987 187 A2065 236 F0004851 285 F0140
41 A1856 90 A1931 139 A1988 188 A2066 237 F0004395 286 F0138
42 A1857 91 A1932 140 A1989 189 A2071 238 F0004918 287 F0125
43 A1858 92 A1933 141 A1994 190 A2072 239 F0615 288 F0118
44 A1859 93 A1934 142 A1995 191 A2074-3 240 F0617 289 F0041
45 A1860-1 94 A1935 143 A1996 192 A2075-2 241 F0618 290 F0043
46 A1861 95 A1936 144 A1997 193 A2077 242 F0624 291 F0045
47 A1862 96 A1937-1 145 A1998 194 A2078 243 F0652 292 F0060
48 A1863 97 A1938 146 A1999 195 A2079-1 244 F0679 293 F0069
49 A1864-1 98 A1939 147 A2000 196 A2080 245 F0682 294 F0023
295 F0025 325 F0153 355 F1609 385 F0004344-菜12 415 F0004411-菜42 445 F0004474-菜72
296 F0034 326 F0154 356 F1623 386 F0004345-菜13 416 F0004412-菜43 446 F0004476-菜73
297 F0039 327 F0155 357 F2368 387 F0004346-菜14 417 F0004413-菜44 447 F0004480-菜74
298 F0040 328 F0193 358 F2370 388 F0004347-菜15 418 F0004414-菜45 448 F0004482-菜75
299 F0004 329 F0014 359 F0669 389 F0004349-菜16 419 F0004415-菜46 449 F0004486-菜76
300 F0005 330 F0016 360 F0675 390 F0004350-菜17 420 F0004416-菜47 450 F0004487-菜77
301 F0009 331 F0017 361 F0697 391 F0004351-菜18 421 F0004417-菜48 451 F0004488-菜78
302 F0010 332 F0020 362 F0680 392 F0004352-菜19 422 F0004418-菜49 452 F0004489-菜79
303 F0011 333 F0022 363 F0626 393 F0004353-菜20 423 F0004419-菜50 453 F0004493-菜80
304 F0616 334 F1532 364 F0650 394 F0004354-菜21 424 F0004420-菜51 454 F0004494-菜81
305 F0627 335 F1536 365 F0105 395 F0004355-菜22 425 F0004421-菜52 455 F0004495-菜82
306 F0629 336 F1538 366 F0106 396 F0004356-菜23 426 F0004422-菜53 456 F0004496-菜83
307 F0637 337 F1539 367 F0112 397 F0004357-菜24 427 F0004423-菜54 457 F0004497-菜84
308 F0641 338 F1552 368 F0113 398 F0004358-菜25 428 F0004424-菜55 458 F0004499-菜85
309 F1200 339 F2371 369 F0646 399 F0004360-菜26 429 F0004426-菜56 459 F0005083-菜86
310 F1228 340 F2373 370 F0651 400 F0004361-菜27 430 F0004428-菜57 460 F0005150-菜87
311 F1236 341 F2374 371 F0655 401 F0004364-菜28 431 F0004430-菜58 461 F0005161-菜88
312 F1237 342 F2375 372 F0663 402 F0004365-菜29 432 F0004431-菜59 462 F0005171-菜89
313 F1297 343 F2376 373 F0664 403 F0004366-菜30 433 F0004433-菜60 463 F0005176-菜90
314 F1136 344 F1298 374 F0002693-菜1 404 F0004367-菜31 434 F0004434-菜61 464 F0005184-菜91
315 F1138 345 F1345 375 F0004331-菜2 405 F0004368-菜32 435 F0004440-菜62 465 F0005199-菜92
316 F1148 346 F1361 376 F0004332-菜3 406 F0004369-菜33 436 F0004442-菜63 466 F0005201-菜93
317 F1156 347 F1432 377 F0004333-菜4 407 F0004370-菜34 437 F0004448-菜64 467 F0005203-菜94
318 F1162 348 F1435 378 F0004335-菜5 408 F0004401-菜35 438 F0004451-菜65 468 F0005205-菜95
319 F1094 349 F0642 379 F0004336-菜6 409 F0004403-菜36 439 F0004457-菜66 469 F0005211-菜96
320 F1095 350 F0643 380 F0004337-菜7 410 F0004404-菜37 440 F0004458-菜67 470 F0005216-菜97
321 F1100 351 F0645 381 F0004338-菜8 411 F0004405-菜38 441 F0004465-菜68 471 F0005218-菜98
322 F1117 352 F0648 382 F0004340-菜9 412 F0004406-菜39 442 F0004470-菜69 472 F0005219-菜99
323 F1120 353 F0649 383 F0004343-菜10 413 F0004408-菜40 443 F0004472-菜70 473 F0005220-菜100
324 F0152 354 F1600 384 F0004343-菜11 414 F0004409-菜41 444 F0004473-菜71

Table 2

Grading and evaluation standard of A.obtectus resistance of common bean resources"

分级
Grade		 受害率
Infestation rate (%)		 抗性评价
Resistant evaluation
1 0.0~10.0 高抗（HR）
3 10.1~35.0 抗（R）
5 35.1~65.0 中抗（MR）
7 65.1~90.0 感（S）
9 > 90.0 高感（HS）

Fig.1

Infection process of A.obtectus"

Fig.2

Infection status of common bean resources with different resistance levels to A.obtectus"

Fig.3

Percentage of common bean germplasms with different resistant grades"

Table 3

High-resistant common bean germplasms to A.obtectus"

品种（系）
Variety (line)		 受害率
Infestation rate (%)		 严重度
Severity
F1432 0 0
F0645 0 0
F0675 0 0
F0004351 0 0
F0004406 0 0
F0004353 1 1
A2054 4 4
F0004352 4 11
F0106 5 5
F0004357 6 9
F0004465 6 22
A1948 7 46
F0004404 8 13
F0004405 9 9
A1823 9 9

Table 4

Percentage of resistant common bean resources with different grain colors and their total grain colors"

粒色
Grain
color		 高抗HR 抗R 中抗MR 感S 高感HS
数量
Number		 占比
Percentage
(%)		 数量
Number		 占比
Percentage
(%)		 数量
Number		 占比
Percentage
(%)		 数量
Number		 占比
Percentage
(%)		 数量
Number		 占比
Percentage
(%)
红色Red 6 4.8 23 18.3 42 33.3 34 27.0 21 16.6
花斑Piebald 3 2.2 8 6.0 42 31.3 58 43.3 23 17.2
白色White 2 2.5 12 14.8 25 30.9 27 33.3 15 18.5
黑色Black 1 2.3 9 20.9 13 30.3 16 37.2 4 9.3
浅黄Light yellow 2 3.9 3 5.9 20 39.2 16 31.4 10 19.6
褐色Brownness 1 2.6 5 13.2 15 39.5 11 28.9 6 15.8

Fig.4

Percentage of resistance resources in different colored common bean resources"

[1] 申智慧, 刘春, 杨洪, 等. 菜豆象的发生与防治. 植物医生, 2014, 27(2):46-47.
[2] 莫仁浩, 吴佳教. 菜豆象入侵风险及检疫对策探讨. 粮食储藏, 2006, 35(6):13-15.
[3] 番启山, 焦晓品, 李生贵, 等. 菜豆象的生物学及防治初步研究. 植物检疫, 1994, 8(3):135-142.
[4] 汪兆春, 张忠民, 耿坤, 等. 菜豆象入侵我国的风险性评价. 贵州农业科学, 2018, 46(1):47-50.
[5] 赵龙, 张时龙, 吴宪志, 等. 贵州省菜豆象疫情普查. 现代农业科技, 2015(22):126-127.
[6] 王菊平, 王小奇, 高萍, 等. 菜豆象在中国的风险性分析. 中国植保导刊, 2006, 26(5):8-11.
[7] 张生芳, 刘永平. 菜豆象幼虫的形态结构及11种豆象幼虫检索表. 植物检疫, 1995, 10(2):92-96.
[8] 全尚维, 吕杰. 菜豆象检验鉴定及处理研究. 中国进出境动植检, 1997(1):31-33.
[9] 李斌, 陈树良, 秦超, 等. 普通菜豆种质资源研究现状及进展. 农业科学与技术, 2016, 17(11):2572-2577.
[10] 赵阳, 王艳, 林雪丹, 等. 贵州芸豆虫害菜豆象的调查与鉴定. 农技服务, 2021, 38(5):60-62.
[11] Rodriguez G A, Casquero P A, Cardoza R E, et al. Effect of trichodiene synthase encoding gene expression in Trichoderma strains on their effectiveness in the control of Acanthoscelides obtectus. Journal of Stored Products Research, 2019,83:275-280.
[12] 李南植, 宋保深, 吕杰, 等. 环境温湿度对菜豆象成虫寿命及产卵影响的研究. 植物检疫, 1995, 9(4):193-197.
[13] 刘旭明, 金达生, 程须珍, 等. 绿豆种质资源抗豆象鉴定研究初报. 作物品种资源, 1998(2):35-37.
[14] 仲伟文, 杨涛, 段灿星, 等. 豌豆种质资源抗绿豆象鉴定. 作物杂志, 2014(5):43-47.
[15] 李晓明. 普通菜豆抗菜豆象基因发掘及其抗虫机制研究. 北京: 中国农业科学院, 2022.
[16] 王强, 邓永学, 王进军, 等. 绿豆象西昌种群和沈阳种群的杂交研究. 西南大学学报(自然科学版), 2008, 30(2):133-137.
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