Crops ›› 2021, Vol. 37 ›› Issue (2): 57-61.doi: 10.16035/j.issn.1001-7283.2021.02.008

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Preliminary Study on Inheritance of Haploid Natural Double Pollen Seeding Ability in Maize

Li Zhongnan1(), Wang Yueren2, Wu Shenghui2, Liu Liwei3, Qu Haitao2, Sun Zhenyu1, Li Guangfa2()   

  1. 1Agricultural and Technology Extension Station of Jilin Province, Changchun 130033, Jilin, China
    2Tonghua Academy of Agricultural Sciences of Jilin Province, Meihekou 135007, Jilin, China
    3Introduction and Breeding Center of New Crop Variety of Jilin Province, Changchun 130033, Jilin, China
  • Received:2020-03-19 Revised:2020-05-20 Online:2021-04-15 Published:2021-04-16
  • Contact: Li Guangfa E-mail:18741379479@163.com;lgftn666@sina.com

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Abstract:

The number of seeds of haploid natural double selfing ear induced by F1 of 15D752×15D435 and PH6WC×29 as the research objects in maize. The population genetic model of DH and the method of mixed distribution were used to study the pollen seeding ability. The results showed that the ability of haploid natural doubled pollen seeding was determined by four major genes, which had additive and epistatic effects and the heritability of major genes were 95.85% and 83.67%, respectively.

Key words: Maize, Haploid, Natural double, Pollen seeding ability, Inheritance

Table 1

Germplasm origins and characteristics of inbred lines and DH lines"

材料Material 种质Germplasm 来源Origin 穗轴色Ear cob color 雄穗分枝数Male branch number
15D752 PH4CV×A6,兰卡斯特×（美杂×丹340） 中国（自选） 红 5~7
15D435 PH4CV×A6,兰卡斯特×（美杂×丹340） 中国（自选） 红 4~6
PH6WC PH01N×PH09B, 瑞德 美国 白 0~2
29 掖107×铁7922,瑞德 中国（自选） 白 4~6

Table 2

Percentage of haploid self seeding plants of two populations"

群体
Population		 单倍体株数
Haploid plants
number		 授粉株数
Pollination plants
number		 授粉率
Pollination rate
(%)		 结实株数
Seeding plants
number		 授粉结实株率
Pollination seeding
plants rate (%)		 结实株率
Seeding plants
rate (%)
15D752×15D435 1772 428 24.15 191 44.63 10.78
PH6WC×29 43656 2069 4.74 319 15.42 0.73

Table 3

Characteristic values of open ear and selfing ear"

群体
Population		 开放穗粒数Open ear seed number 结实株率
Seeding plants
rate (%)		 变异系数
CV (%)		 P 正态分布
Normal
distribution
平均值
Average value		 最大值
Maximum value		 最小值
Minimum value
15D752×15D435 18.40 37.00 3.00 100 61.07 0.7753 是
PH6WC×29 3.74 18.00 0.00 75 109.92 0.0000 否
群体
Population		 自交穗粒数Selfing ear seed number 授粉结实株率
Pollination seed plants rate (%)		 变异系数
CV (%)		 P 正态分布
Normal distribution
平均值
Average value		 最大值
Maximum value		 最小值
Minimum value
15D752×15D435 4.19 54.00 0.00 44.63 196.92 0.0000 否
PH6WC×29 0.53 44.00 0.00 15.42 434.41 0.0000 否

Table 4

AIC value of seed number per selfing ear of two populations"

模型Model AIC值 AIC value
15D752×15D435 PH6WC×29
0MG 11730.5009 9345.8354
1MG-A 11732.5058 9347.8389
2MG-AI 11714.5854 9346.7849
2MG-A 11734.5072 9349.8400
2MG-EA 11732.5072 9347.8400
2MG-DominanceI 11734.5059 9349.8390
2MG-RecessiveI 11712.5853 9344.7849
2MG-Additive 11712.5854 9344.7849
2MG-Complementary 11710.5854 9342.7849
2MG-Duplicate 11732.5027 9347.8368
2MG-Inhibiting 11732.5027 9347.8368
3MG-AI 9155.8195 7436.5910
3MG-A 11736.5079 9351.8406
3MG-CEA 11741.2412 9389.2766
3MG-PEA 11734.5082 9349.8408
4MG-AI 6421.9120 6509.2943
4MG-A 11738.5007 9353.8359
4MG-CEA 11732.5089 9347.8414
4MG-EEA 11734.5081 9349.8409
4MG-EEEA 10919.9731 8503.5249

Table 5

Test for goodness of optimal models"

群体
Population		 统计量Statistic
U12 U22 U32 nW2 Dn
15D752×15D435 5.1908*
(0.0227)		 136.1232**
(0.0000)		 1432.231**
(0.0000)		 129.656
(0.3031)		 0.0005
(1)
PH6WC×29 6.4027*
(0.0114)		 113.299**
(0.0000)		 1074.32**
(0.0000)		 106.2457
(0.2754)		 0.0005
(1)

Table 6

Estimate values of genetic parameters"

遗传参数
Genetic parameter		 估计值Estimate value
15D752×15D435 PH6WC×29
m 6.5963 3.9567
da 4.0027 1.8050
db 2.6193 1.8050
dc 4.0344 2.1318
dd 2.3993 1.6856
iab 2.6193 1.8050
iac 4.0344 2.1318
iad 2.3993 1.6856
ibc 2.6509 2.1318
ibd 2.4376 1.6856
icd 2.4277 1.9842
σ2p 16.9509 5.3535
σ2mg 16.2468 4.4793
σ2 0.7041 0.8742
h2mg (%) 95.8500 83.6700

Table 7

Distribution of sixteen genotypes of ear seed number from 15D752×15D435 population"

穗粒数分布Distribution of ear seed number 基因型（效应值）Genotype (effect value)
0 aabbCCdd (-0.05)、aaBBccDD (-0.05)、aabbCCDD (-0.07)、aaBBccdd (-0.07)、AAbbccDD (-0.12)、AAbbccdd (0.01)、AABBccdd (0.31)、aaBBCCdd (0.38)、aabbccDD (0.38)
5 AAbbCCdd (5.99)
9 AABBccDD (9.93)
10 aaBBCCDD (10.11)、aabbccdd (10.11)
15 AAbbCCDD (15.57)
16 AABBCCdd (16.89)
36 AABBCCDD (36.22)

Table 8

Distribution of sixteen genotypes of ear seed number from PH6WC×29 population"

穗粒数分布Distribution of ear seed number 基因型（效应值）Genotype (effect value)
0 AABBccdd (-0.10)、aabbCCdd (-0.28)、aaBBccDD (-0.28)、AAbbccDD (-0.28)、aaBBccdd (0.32)、AAbbccdd (0.32)、aabbCCDD (0.32)、aaBBCCdd (0.61)、aabbccDD (0.61)、AAbbCCdd (0.61)
6 AABBccDD (6.05)
7 AAbbCCDD (7.95)、aaBBCCDD (7.95)、aabbccdd (7.95)
8 AABBCCdd (8.73)
22 AABBCCDD (22.81)
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