Crops ›› 2021, Vol. 37 ›› Issue (6): 122-128.doi: 10.16035/j.issn.1001-7283.2021.06.019

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Effects of Crop Rotation on Soil Microbial Community in Sweet Potato Field

Liu Yajun(), Wang Wenjing, Wang Honggang, Wang Qi, Hu Qiguo(), Chu Fengli   

  1. Shang qiu Academy of Agriculture and Forestry Sciences, Shangqiu 476000, Henan, China
  • Received:2020-12-21 Revised:2021-02-23 Online:2021-12-15 Published:2021-12-16
  • Contact: Hu Qiguo E-mail:liuyajun0812@163.com;huqiguo1234@126.com

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Abstract:

In order to alleviate the obstacle of sweet potato continuous cropping, we explored the effects of different rotation patterns (sweet potato continuous cropping, sweet potato-peanut rotation and sweet potato-wheat rotation) on soil microbial community by PLFA and biology ecological plate method, and the changes of soil microbial community structure, the utilization ability of carbon source and diversity index under the different rotation patterns of sweet potato were determined. The main results were as follows: compared with the continuous cropping of sweet potato, sweet potato-peanut rotation significantly (P<0.05) improved the contents of the biomass of soil microbial bacteria, actinomycetes, Gram-positive bacteria and Gram-negative bacteria, reduced the biomass of fungi and the ratio of fungi to bacteria, sweet potato-wheat rotation reduce the biomass of soil microbial bacteria, actinomycetes, fungi, Gram-positive bacteria and Gram-negative bacteria, and improved the ratio of fungi to bacteria. Sweet potato-peanut rotation improved the utilization ability of carboxylic acid compound, carbohydrate, amino acid and amine compound in soil microorganism, sweet potato-wheat rotation improved the utilization ability of carbohydrate, amino acid and amine compound in soil microorganism, the utilizations of carboxylic acid compounds, aromatic compounds and carbohydrates in sweet potato-peanut treatment was significantly higher than that in sweet potato-wheat treatment; Sweet potato-peanut and sweet potato-wheat rotation improved the Shannon index, Mclntoch index and Simpson index, The Mclntoch index of the sweet potato-peanut treatment was higher than that of sweet potato-wheat treatment, while the Shannon index and Simpson index decreased significantly; The redundancy analysis (RDA) showed that there were promotion or restriction relationships among the soil microbial community structure, the utilization ability of carbon source and diversity index with the soil nutrients. In conclusion, the rotation of sweet potato -peanut or -wheat can change the structure and functional diversity of soil microbial community.

Key words: Sweet potato, Rotation, Soil microorganism, Phospholipid fatty acid, Utilization ability of carbon source, Diversity index

Table 1

Effects of different rotation patterns on the main soil microbial community nmol/g"

处理
Treatment		 真菌
Fungal		 细菌
Bacterial		 放线菌
Actinomyc-ete		 真菌/细菌
Fungal/bacterial		 革兰氏阳性菌（G+）
Gram positive		 革兰氏阴性菌（G-）
Gram negative		 G+/G-
甘薯―甘薯S-S 4.12±0.36a 10.76±0.73b 2.48±0.25b 0.38±0.08a 3.59±0.33b 7.18±0.31b 0.50±0.05a
甘薯―花生S-P 3.35±0.12b 13.63±0.36a 2.69±0.10a 0.25±0.02b 4.90±0.69a 8.73±0.32a 0.57±0.06a
甘薯―小麦S-W 3.97±0.39ab 9.85±0.22b 2.46±0.12b 0.41±0.07a 3.10±0.11b 6.76±0.39b 0.46±0.04a

Table 2

Effects of different rotation patterns on carbon source utilization of soil microbial community"

处理
Treatment		 羧酸类化合物
Carboxylic acids		 多聚化合物
Poly compound		 碳水化合物
Carbohydrates		 芳香化合物
Aromatic		 氨基酸
Amino acid		 胺类化合物
Amine
甘薯―甘薯S-S 1.13±0.10b 0.85±0.16a 0.69±0.07c 0.66±0.07a 0.51±0.04b 0.38±0.06b
甘薯―花生S-P 1.25±0.06a 0.79±0.13a 1.68±0.11a 0.62±0.05a 1.26±0.11a 0.89±0.07a
甘薯―小麦S-W 0.76±0.07c 0.66±0.04a 1.42±0.14b 0.26±0.03b 1.11±0.08a 0.87±0.16a

Table 3

Effects of different rotation patterns on soil microbial diversity indexes"

处理
Treatment		 丰富度指数
Shannon index		 均匀度指数
Mclntoch index		 优势度指数
Simpson index
甘薯―甘薯S-S 1.79±0.28c 4.39±0.47b 43.64±1.24c
甘薯―花生S-P 2.54±0.12b 8.74±0.43a 48.57±2.12b
甘薯―小麦S-W 2.87±0.15a 7.97±0.73a 63.07±1.47a

Fig.1

The RDA analysis between soil microbial community structure (a), carbon source utilization capacity(b), diversity index (c)and environmental factors The AP, AK, AN, TN, TP, OM and pH represent the soil available phosphorus, available potassium, alkali hydrolyzable nitrogen, total nitrogen, total phosphorus, organic matter and pH value, respectively; The F/B, bacterial, actinomycete, fungal, G+, G-, G+/C- represent the fungi/bacteria ratio, soil fungi, bacteria, actinomycete, Gram-positive bacteria, Gram-negative bacteria, Gram-positive bacteria / Gram-negative bacteria ratio, respectively; The carboh, pol, carbox, phe, amino, amine represent the carbohydrates, polymers, carboxylic acids, aromatic compounds, amino acids, amines, The Shanindx, Mclnindx and Simpindx represent the aroma index, evenness index and Simpson index, respectively. SS1-SS4, SP1-SP4 and SW1-SW4 represent four repeats of sweet potato-sweet potato continuous cropping, sweet potato-peanut rotation and sweet potato- wheat rotation, respectively"

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