Crops ›› 2021, Vol. 37 ›› Issue (6): 22-27.doi: 10.16035/j.issn.1001-7283.2021.06.004

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Effects of Genetic Interaction between DEP1 and NRT1.1B on Nitrogen Use in Rice

Li Xu(), Fu Lidong, Wang Yu, Sui Xin, Ren Hai, Lü Xiaohong, Ma Chang, Du Meng, Mao Ting()   

  1. Liaoning Institute of Saline-Alkali and Utilization, Panjin 124010, Liaoning, China
  • Received:2021-04-23 Revised:2021-06-15 Online:2021-12-15 Published:2021-12-16
  • Contact: Mao Ting E-mail:chinalixu1983@163.com;chinamaoting1985@163.com

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Abstract:

The improvement of nitrogen use efficiency has been the key direction of rice genetic improvement. The erect panicle allele dep1 and indica rice allele nrt1.1b were beneficial to improve the nitrogen use efficiency of rice. Therefore, it was of great significance to clarify the genetic interaction between DEP1 and NRT1.1B genes for the cultivation of nitrogen-efficient rice varieties. In this study, recombinant inbred lines (RILs) carrying different combinations of DEP1 and NRT1.1B genotypes were used as test materials to analyze the effects of genetic interaction between DEP1 and NRT1.1B genes on nitrogen utilization, yield and its components of rice under low (LN), medium (MN) and high nitrogen (HN) conditions. The results showed that under different nitrogen conditions, dep1/nrt1.1b combination had the largest nitrogen harvest index. Under LN condition, the introduction of nrt1.1b gene was conducive to significantly improving nitrogen use efficiency, and the effect of dep1 was more significant under MN and HN conditions. In terms of yield, the average yield of dep1 carrying lines were significantly higher than that of other lines due to the significant increase in effective panicle number and grain number per panicle, while NRT1.1B gene had no significant effect on yield.

Key words: Rice, Nitrogen use efficiency, DEP1 gene, NRT1.1B gene, Genetic interaction

Table 1

Information of primers"

基因
Gene		 引物名称
Primer name		 引物序列（5′-3′）
Primer sequence (5′-3′)		 片段长度
Fragment size (bp)
DEP1
DEP1-1
DEP1-1-F: AAAGACCAAGGTGCCTCA
DEP1-1-R: TGGTTCAACCTCGTCTCATA		 466 (dep1 allele)
1091 (DEP1 allele)
NRT1.1B


1nrt/1NRT


NRT1.1B-O-F: GATGGAGGCGATGAGGAAGA
NRT1.1B-O-R: GCTGCCAAGAAACACCACAA
NRT1.1B-I-F: TCGTGCACAGCCTCCACTTGCTCGACG
NRT1.1B-I-R: AGGTCGGCGGCGGAGTCGCCGGCTAT		 243 (nrt1.1b allele)
177 (NRT1.1B allele)
368 (CK)

Fig.1

Number of lines of different combinations of DEP1 and NRT1.1B genes in RILs"

Fig.2

Effects of different combinations of DEP1 and NRT1.1B genes on N contents in RILs Different lowercase letters indicate significant difference at 0.05 level, the same below"

Fig.3

Effects of different combinations of DEP1 and NRT1.1B genes on nitrogen harvest indexes in RILs"

Fig.4

Effects of different combinations of DEP1 and NRT1.1B genes on NR and GS activities in RILs"

Table 2

Effects of different combinations of DEP1 and NRT1.1B genes on yield and its components in RILs"

株系
Line		 单株有效穗数Effective panicles per plant 穗粒数Grains per panicle
LN MN HN LN MN HN
DEP1/NRT1.1B 11±1.31b 13±1.38b 16±1.54b 89±7.80b 109±9.89b 118±10.56b
DEP1/nrt1.1b 12±1.52b 12±1.55b 17±1.59b 90±9.20b 108±11.12b 121±11.81b
dep1/NRT1.1B 14±1.68ab 16±1.92ab 20±2.01ab 108±10.32ab 126±12.11a 135±12.94a
dep1/nrt1.1b 15±1.81a 17±1.89a 21±2.22a 109±11.32a 125±11.35ab 133±10.25ab
株系
Line		 千粒重1000-grain weight (g) 产量Yield (kg/hm2)
LN MN HN LN MN HN
DEP1/NRT1.1B 27.21±1.45ab 27.33±1.56a 27.32±1.89a 6840±281b 9015±312b 9315±332b
DEP1/nrt1.1b 27.24±1.39a 27.25±1.42ab 27.25±1.45ab 6930±279b 9180±333b 9345±321b
dep1/NRT1.1B 24.23±1.14b 25.31±1.31b 25.12±1.36b 7680±235a 9675±321a 10260±356a
dep1/nrt1.1b 24.21±1.21b 25.12±1.45b 25.31±1.41b 7650±242ab 9630±299ab 10200±371ab
[1] 石鑫蕊, 任彬彬, 江琳琳, 等. 有机肥替代部分化肥对水稻光合速率、氮素利用率和产量的影响. 应用生态学报, 2021, 32(1):154-162.
[2] 鲁晨妮, 周雪, 程旺大, 等. 嘉兴地区不同年代粳稻品种产量及氮素利用效率. 中国稻米, 2020, 26(4):93-95.
[3] 杨梢娜, 范国灿, 杨飞, 等. 施肥对滨海平原单季稻秀水134产量和氮素利用率的影响. 浙江农业科学, 2020, 61(6):1078-1080.
[4] 彭少兵, 黄见良, 钟旭华, 等. 提高中国稻田氮肥利用率的研究策略. 中国农业科学, 2002, 35(9):1095-1103.
[5] 钟旭华, 黄农荣, 郑海波. 华南双季杂交稻氮素养分消耗量及其影响因素研究. 植物营养与肥料学报, 2007, 13(4):569-576.
[6] 余友玲, 吴文革, 陈刚, 等. 水稻氮高效品种的初步筛选. 中国稻米, 2015, 21(4):99-102.
[7] 张俊国, 张三元, 杨春刚, 等. 水稻氮高效品种资源筛选的初步研究Ⅱ.不同施氮水平下水稻产量性状的品种间差异. 吉林农业科学, 2009, 34(4):27-30.
[8] Suenaga A, Moriya K, Sonoda Y, et al. Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants. Plant Cell Physiology, 2003, 44(2):206-211.
doi: 10.1093/pcp/pcg017
[9] Tabuchi M, Abiko T, Yamaya T. Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza satiliva L.). Journal of Experimental Botany, 58(9):2319-2327.
doi: 10.1093/jxb/erm016
[10] Ishiyama K, Inoue E, Tabuchi M, et al. Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots. Plant Cell Physiology, 2004, 45(11):1640-1647.
doi: 10.1093/pcp/pch190
[11] Teng S, Tian C G, Chen M S, et al. QTLs and candidate genes for chlorate resistance in rice (Oryza sativa L.). Euphytica, 2006, 152:141-148.
doi: 10.1007/s10681-006-9189-1
[12] Fan X R, Feng H M, Tan Y W, et al. A putative 6-transmembrane nitrate transporter OsNRT1.1b plays a key role in rice under low nitrogen. Journal of Integrative Plant Biology, 2016, 58(6):590-599.
doi: 10.1111/jipb.12382
[13] Hu B, Wang W, Ou S J, et al. Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies. Nature Genetics, 2015, 47(7):834-838.
doi: 10.1038/ng.3337
[14] Sun H Y, Qian Q, Wu K, et al. Heterotrimeric G proteins regulate nitrogen-use efficiency in rice. Nature Genetics, 2014, 46(4):652-656.
doi: 10.1038/ng.2958
[15] Huang X, Qian Q, Liu Z, et al. Natural variation at the DEP1 locus enhances grain yield in rice. Nature Genetics, 2009, 41(4):494-497.
doi: 10.1038/ng.352
[16] Yan C J, Zhou H J, Yan S. Identification and characterization of a major QTL responsible for erect panicle trait in japonica rice (Oryza sativa L.). Theoretical and Applied Genetics, 2007, 115:1093-1100.
doi: 10.1007/s00122-007-0635-9
[17] Zhou Y, Zhu J Y, Li Z Y, et al. Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication. Genetics, 2009, 183(1):315-324.
doi: 10.1534/genetics.109.102681
[18] 徐正进, 陈温福. 中国北方粳型超级稻研究进展. 中国农业科学, 2016, 49(2):239-250.
[19] 钮中一, 杨志海, 李建华, 等. 武运粳7号的特征特性和高产栽培技术. 中国稻米, 1998(4):9-10.
[20] 杨贤莉, 王嘉宇, 刘丹, 等. 沈农系列水稻高产品种及其衍生系的遗传结构分析. 中国水稻科学, 2014, 28(5):496-502.
[21] 方琳, 陶亚军, 张灵, 等. 水稻氮高效基因NRT1.1B功能标记开发和资源筛选. 分子植物育种, 2020, 18(23):7795-7800.
[22] 赵明珠. 水稻DEP1调控产量及其与NRT1.1B聚合效应. 沈阳:沈阳农业大学, 2017.
[23] 杨守仁, 张龙步, 陈温福, 等. 水稻超高产育种的理论和方法. 作物学报, 1996, 22(3):295-304.
[24] 袁隆平, 武小金, 颜应成, 等. 水稻广谱广亲和系的选育策略. 中国农业科学, 1997, 30(4):1-8.
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