Crops ›› 2021, Vol. 37 ›› Issue (6): 58-61.doi: 10.16035/j.issn.1001-7283.2021.06.009

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Identification of New Heading Date QTLs Using High Density Genetic Map in Rice

Su Daiqun1(), Chen Liang1, Li Feng1, Wu Qi1, Bai Junjie2, Zou Detang2, Wang Jingguo2, Liu Hualong2(), Zheng Hongliang2   

  1. 1Heilongjiang Province Seed Industry Technical Service Center, Harbin 150008, Heilongjiang, China
    2Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region,Ministry of Education, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
  • Received:2021-03-26 Revised:2021-06-22 Online:2021-12-15 Published:2021-12-16
  • Contact: Liu Hualong E-mail:sudaiqun@163.com;liuhualongneau@163.com

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Abstract:

Heading date is the key factor to determine the rice planting area and its seasonal adaptability, so it is very important to find new major QTLs controlling rice heading date. The high-density genetic linkage map with 527 bin markers of the combinant inbred line (RIL) population derived from 'Kongyu 131/Xiaobaijingzi' was used for genotype analysis of heading date in rice by genotyping by target sequencing (GBTS). Through the basic statistical analysis of the parents and the RIL population, it was found that the parent's heading date was extremely significantly different, the phenotypes were within the range of the RIL population, and there was an obvious phenomenon of transgressive segregation that conformed to the normal distribution. Four QTLs were detected on chromosomes 1, 3, and 7 of rice using full interval mapping method of IciMapping 4.2. Among them, three QTLs contained known genes OsGI, Hd6 and Ghd7 related to heading stage, respectively. qHD-3-1 was a new QTL controlling the heading date of rice.

Key words: Rice, Heading date, Bin marker, QTL, Genotyping by target sequencing (GBTS), Recombinant inbred lines

Table 1

Basic statistical analysis of heading stage of rice"

项目
Item		 亲本Parents 重组自交系群体RIL population
空育131
Kongyu 131 (d)		 小白粳子
Xiaobaijingzi (d)		 平均值
Mean (d)		 标准差
Standard deviation		 变异范围
Range (d)		 变异系数
Coefficient of variation (%)		 峰度
Kurtosis		 偏度
Skewness
抽穗期Heading date 91.00 104.00** 97.74 7.51 76.00~111.00 7.68 -0.42 -0.23

Fig.1

Distribution of QTLs on chromosomes at heading stage in rice"

Table 2

Rice heading stage QTLs and its genetic effects"

数量性状位点
QTL		 染色体
Chromosome		 标记区间
Marker interval		 LOD值
LOD peak		 贡献率
Contribution rate (%)		 加性效应
Additive effect		 已知基因
Known gene
qHD-1-1 1 C1_4330799 C1_4645558 3.0181 4.0619 1.5969 OsGI
qHD-3-1 3 C3_799346 C3_1493308 7.0671 10.5098 2.7637
qHD-3-2 3 C3_29189855 C3_33130038 3.2297 4.5338 1.6866 Hd6
qHD-7-1 7 C7_8809828 C7_13201476 13.2288 21.7016 -3.8409 Ghd7
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