Crops ›› 2022, Vol. 38 ›› Issue (2): 69-74.doi: 10.16035/j.issn.1001-7283.2022.02.010

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QTLs Mapping of Leaf Rust Resistance in Wheat Variety Zhoumai 22

Yan Xiaocui1(), Duan Zhenying2, Yang Huali2, Yao Zhanjun2,*(), Li Zaifeng1,*()   

  1. 1College of Plant Protection, Hebei Agricultural University/Biological Control Center for Plant Diseases and Plant Pests of Hebei, Baoding 071001, Hebei, China
    2College of Agronomy, Hebei Agricultural University/Key Laboratory of Research and Utilization of Crop Germplasm Resources in North China, Ministry of Education, Baoding 071001, Hebei, China
  • Received:2021-03-05 Revised:2021-05-11 Online:2022-04-15 Published:2022-04-24
  • Contact: Yao Zhanjun,Li Zaifeng E-mail:yanxiaocui101412@126.com;yzhj201@aliyun.com;lzf7551@aliyun.com

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Abstract:

Leaf rust (LR) is one of the serious fungal diseases of wheat. Planting resistant cultivars is the most effective and safe way to control the disease. Due to the emergence of new pathotypes in the pathogen population, the resistance of the existing leaf rust resistance gene is gradually lost. Therefore, it is necessary to continuously explore and study new resistance sources to control this important disease. In the present study, the wheat variety Zhoumai 22 showed resistance to leaf rust at the adult plant stage. In order to analyze the genetic basis of resistance to leaf rust at the adult plant stage of Zhoumai 22, 255 F2:3 lines were obtained by crossing Zhoumai 22 with Mingxian 169. The resistance to leaf rust was identified at adult plant stage in field in two crop seasons, and the QTL for leaf rust resistance in the population was analyzed by composite interval mapping. The results showed that two QTLs for leaf rust resistance were detected at adult plant stage in the population, which were located on 1BL and 2BS chromosomes respectively. The two QTLs were designated as QLr.hebau-1BL and QLr.hebau-2BS, and they explained the phenotypic variation of 9.62%-11.88% and 16.89%-20.99%, respectively. The two QTLs showed stable resistance to leaf rust, and they were provided by Zhoumai 22. Results based on preliminary genetic mapping indicated that QLr.hebau-2BS is LrZH22, whereas QLr.hebau-1BL is a new leaf rust resistance QTL.

Key words: Wheat, Leaf rust, Adult-plant resistance, QTL mapping

Fig.1

Frequency distributions of the FDS of Zhoumai 22×Mingxian 169 F2:3 lines for leaf rust Arrows mean FDS average values for the parents ‘Zhoumai 22’ and ‘Mingxian 169’"

Table 1

FDS for leaf rust of 255 F2:3 from Zhoumai 22×Mingxian 169 in two years"

年份
Year		 周麦22
Zhoumai 22		 铭贤169
Mingxian 169		 F2:3群体F2:3 population
均值
Mean		 最小值
Min.		 最大值
Max.
2014-2015 5 80 30.5 5 100
2015-2016 5 90 30.9 5 100

Table 2

Information of 55 SSR markers"

标记Marker 染色体Chromosome F引物（5’-3’）Forward primer (5’-3’) R引物（5’-3’）Reverse primer (5’-3’)
Xwmc336 1A GTCTTACCCCGCGATCTGC GCGGCCTGAGCTTCTTGAG
Xbarc148 1A GCGCAACCACAATGTATGCT GGGGTGTTTTCCTATTTCTT
Xbarc61 1B TGCATACATTGATTCATAACTCTCT TCTTCGAGCGTTATGATTGAT
Xwmc134 1B CCAAGCTGTCTGACTGCCATAg AGTATAGACCTCTGGCTCACGG
Xwmc766 1B AGATGGAGGGGATATGTTGTCAC TCGTCCCTGCTCATGCTG
Xcfd48 1B ATGGTTGATGGTGGGTGTTT ATGTATCGATGAAGGGCCAA
Xgpw1170 1B AGATCGTTCATCCGATCTGC CAATCTCAGTTTGATGTCCTTCAG
Xcfd65 1B AGACGATGAGAAGGAAGCCA CCTCCCTTGTTTTTGGGATT
Xcfd59 1B TCACCTGGAAAATGGTCACA AAGAAGGCTAGGGTTCAGGC
Glu-B3 1B GGTACCAACAACAACAACCC GGTACCAACAACAACAACCC
ω-secalin 1B ACCTTCCTCATCTTTGTCCT CCGATGCCTATACCACTACT
Xwmc44 1B GGTCTTCTGGGCTTTGATCCTG TGTTGCTAGGGACCCGTAGTGG
Xbarc81 1B GCGCTAGTGACCAAGTTGTTATATGA GCGGTTCGGAAAGTGCTATTCTACAGTAA
Xwmc31 1B GTTCACACGGTGATGACTCCCA CTGTTGCTTGCTCTGCACCCTT
Xwmc631 1B TTGCTCGCCCACCTTCTACC GGAAACCATGCGCTTCACAC
Xwmc432 1D ATGACACCAGATCTAGCAC AATATTGGCATGATTACACA
Xbarc212 2A GGCAACTGGAGTGATATAAATACCG CAGGAAGGGAGGAGAACAGAGG
Xbarc220 2A CCTCTGCCATAAACATCACCTCTC GGCCTCAACATCATGTGAAAGA
Xwms614 2A GATCACATGCATGCGTCATG TTTTACCGTTCCGGCCTT
Xbarc45 2B CCCAGATGCAATGAAACCACAAT GCGTAGAACTGAAGCGTAAAATTA
Xwms111 2B TCTGTAGGCTCTCTCCGACTG ACCTGATCAGATCCCACTCG
Xbarc91 2B TTCCCATAACGCCGATAGTA GCGTTTAATATTAGCTTCAAGATCAT
Xbarc55 2B GCGGTCAACACACTCCACTCCTCTCTC CGCTGCTCCCATTGCTCGCCGTTA
Xgwm148 2B GTGAGGCAGCAAGAGAGAAA CAAAGCTTGACTCAGACCAAA
Xgwm410 2B GCTTGAGACCGGCACAGT CGAGACCTTGAGGGTCTAGA
Xgwm374 2B ATAGTGTGTTGCATGCTGTGTG TCTAATTAGCGTTGGCTGCC
Xcfd44 2D AAACCCAATGGCTCTCACAC ATGGCCCAATTATGCAACTC
Xwms349 2D GGCTTCCAGAAAACAACAGG ATCGGTGCGTACCATCCTAC
Xwms155 3A CAATCATTTCCCCCTCCC AATCATTGGAAATCCATATGCC
Xbarc12 3A CGACAGAGTGATCACCCAAATATAA CATCGGTCTAATTGTCAATGTA
Xwms389 3B ATCATGTCGATCTCCTTGACG TGCCATGCACATTAGCAGAT
Xbarc147 3B GCGCCATTTATTCATGTTCCTCAT CCGCTTCACATGCAATCCGTTGAT
Xwms397 4A TGTCATGGATTATTTGGTCGG CTGCACTCTCGGTATACCAGC
Xbarc70 4A GCGAAAAACGATGCGACTCAAAG GCGCCATATAATTCAGACCCACAAAA
Xbarc10 4B GCGTGCCACTGTAACCTTTAGAAGA GCGAGTTGGAATTATTTGAATTAAACAAG
Xwmc68 4B TACACCTCGCGTGTGTAGCCAA GCTCGAATTCTGGCTCGGCAAC
Xwmc419 4B GTTTCGGATAAAACCGGAGTGC ACTACTTGTGGGTTATCACCAGCC
Xbarc20 4B GCGATCCACACTTTGCCTCTTTTACA GCGATGTCGGTTTTCAGCCTTTT
Xbarc1118 4D CGCAGTTGCCTCCCTTGTTAGATGTT CGCTTATTCCTTTCTCATTGGGTTTG
Xbarc155 5A GCGAGTATTGACGTCTTATTTTTGAA GCGTCATGAATTCTAACAATGTGCATA
Xbarc184 5B TTCGGTGATATCTTTTCCCCTTGA CCGAGTTGACTGTGTGGGCTTGCTG
Xwmc376 5B TCTCAACCACCGACTTGTAA ACATGTAATTGGGGACACTG
Xwms371 5B GACCAAGATATTCAAACTGGCC AGCTCAGCTTGCTTGGTACC
Xwms540 5B TCTCGCTGTGAAATCCTATTTC AGGCATGGATAGAGGGGC
Xbarc59 5B GCGTTGGCTAATCATCGTTCCTTC AGCACCCTACCCAGCGTCAGTCAAT
Xwms182 5D TGATGTAGTGAGCCCATAGGC TTGCACACAGCCAAATAAGG
Xgwm 107 6B ATTAATACCTGAGGGAGGTGC GGTCTCAGGAGCAAGAACAC
Xbarc198 6B CGCTGAAAAGAAGTGCCGCATTATGA CGCTGCCTTTTCTGGATTGCTTGTCA
Xwmc469 6D AGGTGGCTGCCAACG CAATTTTATCAGATGCCCGA
Xcfa2240 7A TGCAGCATGCATTTTAGCTT TGCCGCACTTATTTGTTCAC
Xwms282 7A TTGGCCGTGTAAGGCAG TCTCATTCACACACAACACTAGC
Xcfa2019 7A GACGAGCTAACTGCAGACCC CTCAATCCTGATGCGGAGAT
Xbarc65 7B CCCATGGCCAAGTATAATAT GCGAAAAGTCCATAGTCCATAGTCTC
Xwmc273 7B AGTTATGTATTCTCTCGAGCCTG AGTTATGTATTCTCTCGAGCCTG
Xbarc32 7B GCGTGAATCCGGAAACCCAATCTGTG TGGAGAACCTTCGCATTGTGTCATTA

Table 3

QTL for FDS to leaf rust in 255 F2:3 lines from Zhoumai 22/Mingxian 169"

年份
Year		 QTL SSR标记区间
SSR marker interval		 LOD值
LOD value		 表型变异
Phenotypic variation (%)		 加性效应
Add effect		 贡献亲本
Contributing parent
2014-2015 QLr.hbau-1BL Xwmc31-Xwmc631 6.68 9.62 10.17 周麦22
QLr.hbau-2BS Xgwm374-Xbarc55 15.84 20.99 14.09 周麦22
2015-2016 QLr.hbau-1BL Xwmc31-Xwmc631 7.71 11.88 14.56 周麦22
QLr.hbau-2BS Xgwm374-Xbarc55 13.43 16.89 16.50 周麦22

Fig.2

Leaf rust adult-plant resistance QTL on chromosomes 1BL and 2BS in two years"

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