Crops ›› 2022, Vol. 38 ›› Issue (6): 54-60.doi: 10.16035/j.issn.1001-7283.2022.06.007

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Comprehensive Evaluation of Drought Tolerance of Sugar Beet Germplasms at Seedling Stage

Li Wangsheng1,2(), Wang Xueqian1,2, Yin Xilong1,2, Shi Yang1,2, Liu Dali1,2, Tan Wenbo1,2, Xing Wang1,2()   

  1. 1National Medium-Term Repository of Sugar Beet Germplasm, Heilongjiang University, Harbin 150080, Heilongjiang, China
    2College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China
  • Received:2022-04-21 Revised:2022-06-30 Online:2022-12-15 Published:2022-12-21
  • Contact: Xing Wang E-mail:1595764058@qq.com;xyjiayou086@163.com

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Abstract:

In this study, 336 sugar beet germplasm resources were measured at the seedling stage along with 15 phenotypic, physiological and biochemical indices, and the drought tolerance of these resources was thoroughly assessed using the affiliation function method, principal component analysis, cluster analysis and correlation analysis. The results showed that the indicators of leaf dry weight, plant height, root length, leaf fresh weight, root fresh weight, root dry weight, leaf saturated fresh weight and leaf relative water content were significantly decreased, and root to crown ratio, soluble sugar content, soluble protein content and proline content under drought stress were significantly higher than the control. The principal component analysis transformed the 15 single indicators into six composite indicators that could represent 75.95% of the original data information. Cluster analysis divided the 336 sugar beet germplasm resources into five groups, including 16 germplasms with strong drought tolerance, 49 with relatively strong drought tolerance, 109 with medium drought tolerance, 79 with relatively weak drought tolerance and 83 with weak drought tolerance. Correlation analysis showed that embryonic axis diameter, plant height, root length, leaf fresh weight, root fresh weight, root dry weight, leaf dry weight, leaf saturated fresh weight, relative leaf water content and root-shoot ratio were significantly correlated with D values.

Key words: Sugar beet, Germplasm resources, Drought stress, Comprehensive evaluation

Fig.1

Effects of drought stress on indexes of 336 sugar beet germplasm resources"

Table 1

Principal component analysis of various indexes of sugar beet germplasm resources"

指标Index F1 F2 F3 F4 F5 F6
胚轴直径EAD 0.36 0.08 0.14 0.09 0.01 0.10
株高PH 0.29 -0.03 0.09 -0.21 0.13 -0.16
根长RL 0.18 -0.10 0.19 -0.20 -0.06 -0.61
叶鲜重LFW 0.40 0.04 -0.14 0.00 -0.03 0.03
根鲜重RFW 0.38 -0.07 0.21 0.11 0.02 0.13
根干重RDW 0.34 -0.02 0.18 0.19 -0.00 0.20
叶干重LDW 0.37 0.07 -0.04 0.07 -0.02 -0.01
叶片饱和鲜重LSFW 0.40 0.01 -0.10 0.00 -0.04 0.02
叶片相对含水量RLW 0.14 0.17 -0.47 -0.16 0.12 0.15
根冠比RSR -0.11 -0.20 0.56 0.38 0.02 0.23
可溶性糖含量SS content -0.02 0.55 -0.16 0.31 -0.36 -0.10
可溶性蛋白含量SP content -0.05 0.58 0.20 0.13 -0.19 0.14
超氧化物歧化酶活性SOD activity -0.01 0.23 0.30 -0.72 -0.02 0.46
脯氨酸含量Pro content -0.01 0.34 0.38 -0.16 0.02 -0.47
丙二醛含量MDA content -0.03 0.32 0.01 0.17 0.90 -0.04
特征根Characteristic root 5.47 1.66 1.41 1.00 0.94 0.92
累计贡献率Cumulative contribution rate (%) 36.47 47.52 56.88 63.56 69.82 75.95
因子权重Factor weight 0.48 0.15 0.12 0.09 0.08 0.08

Fig.2

Principal component analysis of indexes of 336 sugar beet germplasm resources"

Fig.3

Scatter plot of D values of 336 sugar beet germplasm resources"

Fig.4

Cluster diagram of drought tolerance analysis of 336 sugar beet germplasm resources"

Fig.5

Correlation analysis between D value of drought tolerance measure and drought tolerance coefficient of each index"

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