Crops ›› 2022, Vol. 38 ›› Issue (1): 174-178.doi: 10.16035/j.issn.1001-7283.2022.01.026

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Effects of Melatonin on Yield and AsA-GSH Cycle in Soybean under Drought Stress

Du Xin(), Li Bo, Mao Luxiao, Chen Wei, Zhang Yuxian, Cao Liang   

  1. College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
  • Received:2021-08-15 Revised:2021-09-22 Online:2022-02-15 Published:2022-02-16

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Abstract:

In order to investigate the effect of melatonin on soybean AsA-GSH cycle under drought stress, the soybean variety Suinong 26 was used as material, and three treatments were set up by pot weighing method: normal water supply (CK), drought stress (D) and drought stress with spraying melatonin (D+M). The results showed that the contents of O2-. and H2O2 in soybean leaves under D treatment were significantly increased compared with CK treatment, and the contents of O2-. and H2O2 on the 15th day after drought under D+M treatment were significantly decreased by 18.09% and 17.37%, respectively. On the 10th day after drought, the contents of AsA and GSH in D+M treatment were significantly increased by 22.39% and 15.30% compared with D treatment, respectively. The grain weight per plant of D+M treatment was significantly higher than that of D treatment, and 9.20% higher than that of D treatment. In conclusion, exogenous melatonin could improve the efficiency of AsA-GSH cycle under drought stress and alleviate the oxidative damage of soybean under drought stress.

Key words: Soybean, Melatonin, Yield, AsA-GSH cycle, Drought stress

Fig.1

Effects of melatonin on ROS by-products of soybean under drought stress Different lowercase letters represent significant difference (P < 0.05), the same below"

Fig.2

Effects of melatonin on key enzymes activities of AsA-GSH cycle in soybean under drought stress"

Fig.3

Effects of melatonin on AsA-GSH cycle substances contents in soybean under drought stress"

Table 1

Effects of melatonin on morphological indexes of soybean under drought stress"

处理
Treatment		 株高
Plant height
(cm)		 茎粗
Stem diameter
(mm)		 底荚高
Bottom pod
height (cm)
CK 91.45±0.45a 7.20±0.25a 21.50±1.03a
D 87.42±3.12b 6.80±0.21a 19.50±0.70a
D+M 88.99±2.36ab 6.94±0.19a 19.95±1.12a

Table 2

Effects of melatonin on yield components of soybean under drought stress"

处理
Treatment		 单株荚数
Pod number
per plant		 单株粒数
Grain number
per plant		 百粒重
100-seed
weight (g)		 单株粒重
Grain weight
per plant (g)
CK 17.75±0.14a 38.84±0.25a 19.13±0.71a 9.00±0.40a
D 15.99±0.26c 36.26±0.33c 14.75±0.48c 6.23±0.18c
D+M 16.74±0.14b 37.78±0.19b 16.55±0.49b 6.80±0.52b
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