Crops ›› 2022, Vol. 38 ›› Issue (6): 152-158.doi: 10.16035/j.issn.1001-7283.2022.06.022

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Photosynthetic Physiological Response to Drought Stress in Sugar Beet at Seedling Stage

Yin Xilong1,2(), Shi Yang1,2, Li Wangsheng1,2, Xing Wang1,2()   

  1. 1National Medium-Term Repository of Sugar Beet Germplasm, Heilongjiang University, Harbin 150080, Heilongjiang, China
    2College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China
  • Received:2022-08-30 Revised:2022-10-11 Online:2022-12-15 Published:2022-12-21
  • Contact: Xing Wang E-mail:2567102543@qq.com;xyjiayou_086@163.com

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Abstract:

Drought stress is an important abiotic factor that inhibits the growth and development of sugar beet and affects yield. The drought-tolerant sugar beet germplasm Yi'an No.1 (V1) and the drought-sensitive germplasm 92011/1-6/1 (V2) were used as test materials to investigate the photosynthetic physiological responses of sugar beet seedlings of different drought-tolerant varieties to drought stress. The effects of drought stress on the growth and development, chlorophyll content, and apparent photosynthesis of sugar beet seedlings were investigated. The results showed that the stem diameter, root length, plant height, leaf fresh weight, root fresh weight, leaf dry weight, and root dry weight of the two sugar beet seedlings decreased under drought stress, and the decrease in V1 was less pronounced than V2, and the decrease in V1 was smaller than V2. The net photosynthetic rate, transpiration rate, leaf stomatal conductance and intercellular CO2 concentration of sugar beet seedlings were significantly reduced by drought stress, and V1 was less affected than V2. The response mechanisms of different drought-tolerant sugar beet varieties to drought stress were somewhat different, which could further analyze their drought resistance and provide a theoretical basis for breeding, stress-resistant cultivation and stable yield of sugar beet.

Key words: Sugar beet, Drought stress, Photosynthetic physiology

Table 1

Phenotypic indexes of sugar beet seedlings"

时间
Time (d)		 处理
Treatment		 品种
Variety		 茎粗
Stem
diameter (cm)		 株高
Plant
height (cm)		 根长
Root length
(cm)		 叶鲜重
Leaf fresh
weight (g)		 根鲜重
Root fresh
weight (g)		 叶干重
Leaf dry
weight (g)		 根干重
Root dry
weight (g)
2 CK V1 4.07±0.11a 16.33±0.85a 25.33±0.62a 4.62±0.09a 1.83±0.08a 0.66±0.03a 0.17±0.00a
V2 3.91±0.07a 14.67±0.24a 25.00±0.41a 4.48±0.08a 1.81±0.05a 0.61±0.02ab 0.16±0.00a
DS V1 3.85±0.10a 15.33±0.24a 24.17±0.62a 4.41±0.14a 1.67±0.06a 0.55±0.02b 0.17±0.00a
V2 3.25±0.04b 12.67±0.62b 21.33±0.85b 3.40±0.11b 1.36±0.04b 0.44±0.02c 0.15±0.00b
5 CK V1 5.89±0.07a 20.43±0.76a 31.73±0.61a 12.94±0.57a 4.08±0.51a 0.90±0.06a 0.28±0.01a
V2 5.75±0.06a 19.17±0.24a 30.50±0.41a 13.00±0.72a 3.59±0.18a 0.85±0.02a 0.27±0.02a
DS V1 3.56±0.06b 13.50±0.41b 22.17±0.62b 4.18±0.12b 1.41±0.05b 0.36±0.02b 0.15±0.00b
V2 2.89±0.04c 10.00±0.41c 17.00±0.41c 2.94±0.09b 1.02±0.09b 0.19±0.02c 0.13±0.00b
7 CK V1 8.79±0.18a 24.33±0.47a 37.00±0.82a 16.75±0.53a 5.98±0.13a 1.69±0.10a 0.43±0.02a
V2 8.73±0.18a 23.67±0.24a 36.17±0.62a 16.55±0.17a 5.93±0.10a 1.65±0.07a 0.42±0.02a
DS V1 3.35±0.11b 11.83±0.34b 20.33±0.85b 4.01±0.13b 1.25±0.06b 0.24±0.03b 0.14±0.00b
V2 2.46±0.06c 6.00±0.41c 13.00±0.41c 2.50±0.13c 0.76±0.06c 0.14±0.01b 0.07±0.01c

Fig.1

Total chlorophyll content of sugar beet seedling leaves under normal water conditions Different small letters indicate significant difference (P < 0.05), the same below"

Fig.2

Total chlorophyll content of sugar beet seedling leaves under drought stress"

Fig.3

Total chlorophyll content of V1 sugar beet seedling leaves under different water conditions"

Fig.4

Total chlorophyll content of V2 sugar beet seedling leaves under different water conditions"

Table 2

Apparent photosynthetic index of sugar beet seedling leaves"

时间Time (d) 处理Treatment 品种Variety Pn [μmol/(m2·s)] Tr [mmol/(m2·s)] Gs [mmol/(m2·s)] Ci (μmol/mol)
2 CK V1 20.51±0.22a 1.24±0.07a 70.94±4.14bc 862.77±30.84a
V2 17.90±0.24b 1.35±0.23a 94.21±23.87ab 636.90±16.52b
DS V1 18.58±0.13c 1.49±0.09a 111.60±8.34a 440.97±1.13c
V2 11.68±0.18d 0.75±0.01b 38.80±1.22c 317.57±15.77d
5 CK V1 25.14±0.02a 1.72±0.16a 148.92±33.20a 900.27±23.73a
V2 24.28±1.18a 1.81±0.19a 170.69±20.55a 864.90±47.15a
DS V1 17.13±0.04b 0.69±0.03b 36.49±2.57b 406.00±43.93b
V2 6.18±0.19c 0.36±0.02c 17.99±1.12b 259.97±12.42c
7 CK V1 31.51±0.08a 2.56±0.02a 454.71±13.95a 1336.70±66.56a
V2 30.03±0.08b 1.91±0.15b 223.28±43.64b 1121.30±96.12b
DS V1 15.43±0.10c 0.57±0.01c 35.67±0.55c 380.13±16.82c
V2 1.04±0.08d 0.20±0.01d 8.99±0.38c 173.90±7.45d
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