Crops ›› 2022, Vol. 38 ›› Issue (6): 98-104.doi: 10.16035/j.issn.1001-7283.2022.06.014

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Effects of GA3 on Root Growth and Osmotic Regulation of Lübaonuo Broomcorn Millet Seedlings under Salt Stress

Wang Jinxiang(), Wang Yanzhi, Xing Lixuan, Liu Jianxia, Wang Runmei   

  1. College of Agriculture and Life Science, Shanxi Datong University/Protected Agricultural Technology Development Center, Datong 037009, Shanxi, China
  • Received:2021-08-17 Revised:2021-09-20 Online:2022-12-15 Published:2022-12-21

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Abstract:

The present study was designed to investigate the effects of salt stress on plant seedling growth, the content of osmotic regulation substance and root activity, and the protective role of application of GA3 against osmotic stress caused by NaCl using hydroponic method in Lübaonuo millet. The results showed that compared with control, salt stress significantly inhibited the growth of seedling and inhibitory effect on root was greater than that on bud. Under salt stress, root elongation was strongly inhibited, soluble protein and free proline contents were increased, and root activity significantly decreased. The results also showed that GA3+NaCl significantly increased the volume of cells in elongation region and the content of free proline, alleviated osmotic stress induced by salt, improved the activity of root and the growth of seedling compared with NaCl treatment. The observations suggested that GA3 could promote the growth of Lübaonuo millet seedlings under salt stress and improve salt-tolerance.

Key words: Broomcorn millet, Salt stress, GA3, Root tip cells, Osmotic regulation, Root activity

Fig.1

Effects of salt stress on seedlings growth of Lübaonuo broomcorn millet (a): Effects of salt stress on seedlings growth (on the 7th day of seedling growth). The different letters indicate the significant difference (P < 0.05), the same below"

Fig.2

Effects of salt stress on cell growth in root tip elongation zone of Lübaonuo broomcorn millet seedling (a): cell structure of root tip elongation zone treated with salt, (b): the aspect radio of cell in root tip elongation zone treated with salt. The results were presented as mean values±S.E. (n=10), the same below"

Fig.3

Effects of salt stress on the contents of soluble sugar, soluble protein and proline in roots of Lübaonuo broomcorn millet seedling"

Fig.4

Effects of salt stress on root activity of Lübaonuo broomcorn millet seedling"

Fig.5

Effects of application of GA3 on the growth of Lübaonuo broomcorn millet seedlings under salt stress (a): alleviating effects of different GA3 concentration on seedlings growth under salt stress, (b): effects of GA3 on seedlings growth under salt stress"

Fig.6

Effects of application of GA3 on cell growth in root tip elongation zone of Lübaonuo broomcorn millet seedlings under salt stress"

Fig.7

Effects of application of GA3 on the contents of soluble sugar, soluble protein and free proline in roots of Lübaonuo broomcorn millet seedling under salt stress"

Fig.8

Effects of application of GA3 on root activity of Lübaonuo broomcorn millet seedling under salt stress"

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