Crops ›› 2019, Vol. 35 ›› Issue (6): 27-32.doi: 10.16035/j.issn.1001-7283.2019.06.005

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The Phenotypic Variations and GA Sensitivity of a Dwarf Mutant d93090 in Foxtail Millet

Zhao Lijuan1,Yuan Hongmei2,Zhao Liwei3,Guo Wendong4,Li Zhijiang1,Li Xiangyu1,Ma Jinfeng1,Li Yandong1,Song Weifu1,Yang Xuefeng1,Liu Dongjun1   

  1. 1Crop Resources Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang,China
    2Industrial Crops Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086,Heilongjiang, China
    3Comprehensive Agricultural Technology Service Center, Yangshu Town Government of Acheng District, Harbin 150314, Heilongjiang, China
    4Nature and Ecology Institute, Heilongjiang Academy of Sciences, Harbin 150040, Heilongjiang,China
  • Received:2019-05-17 Revised:2019-10-12 Online:2019-12-15 Published:2019-12-11

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Abstract:

Abstact A stably inherited dwarf mutant d93090 was obtained from a wild type tall line 93090 in foxtail millet induced by 60Co-γ irradiation. Then its dwarfing morphology and the characteristics response to gibberellin were analyzed. The results showed: Compared with the wild type, the plant height of the dwarf mutant was about 60% of that of the wild type. And other features, such as darker leaves in color, slightly sloping stem, the same stem nodes and delayed flowering time of about 3-5 days were also observed. The seedling length, the length of second leaf sheath and hypocotyl length at seedling stage were sensitive to exogenous GA3. While exogenous GA3 was sprayed at jointing stage, plant height of dwarf mutant was partially restored. The content of endogenous GAl in dwarf mutant was significantly lower than that in wild type. It can be concluded this mutant is a semi-dwarf mutant and is related to the GA metabolic pathway. This study provides new germplasm for dwarf breeding in foxtail millet.

Key words: Foxtail millet, Semi-dwarf mutant, Phenotype, GA3 sensitivity

Table 1

Plant height, leaf numbers, leaf length and width of d93090 and wild type at early jointing stage cm"

材料
Meterial		 株高
Plant height		 叶片数
Leaf number		 第4叶长
4th leaf length		 第5叶长
5th leaf length		 第6叶长
6th leaf length		 第4叶宽
4th leaf width		 第5叶宽
5th leaf width		 第6叶宽
6th leaf width
WT 44.5±4.8 11.9±0.6 49.3±2.4 45.5±1.7 39.7±3.2 2.32±0.2 2.18±0.2 2.03±0.1
d93090 33.4±2.2** 11.4±0.5 46.0±2.0** 42.4±1.3** 36.4±1.8** 2.33±0.1 2.20±0.1 2.00±0.1

Table 2

Plant height, leaf numbers, leaf length and width of d93090 and wild type at flowering stage cm"

材料
Meterial		 株高
Plant height		 叶片数
Leaf number		 第1叶长
1st leaf length		 第2叶长
2nd leaf length		 第3叶长
3rd leaf length		 第2叶宽
2nd leaf width		 第3叶宽
3rd leaf width
WT 175.5±4.50 13.2±0.40 39.20±2.60 41.85±2.30 44.35±2.40 2.78±0.20 2.57±0.05
d93090 107.0±1.60** 12.8±0.60 40.17±2.00 45.36±2.20** 47.30±1.40** 2.95±0.01 2.77±0.10

Table 3

Comparition of plant height and panicle characters of d93090 and wild type at mature stage"

材料
Meterial		 株高(cm)
Plant height		 穗长(cm)
Panicle length		 穗粗(cm)
Panicle diameter		 穗重(g)
Panicle weight		 穗粒重(g)
Grain weight per panicle		 千粒重(g)
1000- seed weight
WT 176.05±4.4 18.5±1.4 2.4±0.2 20.9±3.4 18.45±3.1 1.67±0.1
d93090 107.25±1.7** 24.1±0.8** 2.3±0.2 20.2±2.2 17.20±2.0 1.67±0.1

Fig.1

Internode length of each stem node of wild type and d93090 at mature stage"

Fig.2

Internode length changes of corresponding stem node of wild type and d93090 at mature stage"

Fig.3

Variations induced by different concentrations of GA3, BR and IAA in the length, the second sheath length and hypocotyl length of seedlings"

Fig.4

Recovery of plant height after spraying plant hormone in greenhouse "***" represents P<0.001, the same below"

Fig.5

Content of endogenous GA1 in d93090 and wild type at different growth stages "**" represents P<0.01"

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