Crops ›› 2023, Vol. 39 ›› Issue (3): 134-138.doi: 10.16035/j.issn.1001-7283.2023.03.018

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Effects of Foliar Ornithine Spraying at Heading Stage on Yield, Quality and 2-Acetyl-1-Pyrroline Biosynthesis of Fragrant Rice

Xing Pipeng(), Huang Yanfeng, Yi Siyuan, Lan Rujian, Pan Shenggang, Mo Zhaowen, Tian Hua, Duan Meiyang, Tang Xiangru()   

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agricultural Bioresources, South China Agricultural University/Scientific Observing and Experimental Station of Crop Cultivation in South China, Ministry of Agriculture and Rural Affairs/Guangzhou Key Laboratory for Science and Technology of Aromatic rice, Guangzhou 510642, Guangdong, China
  • Received:2021-11-14 Revised:2021-12-01 Online:2023-06-15 Published:2023-06-16

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Abstract:

In order to explore the effect of foliar ornithine spraying at the heading stage on the yield, quality and 2-acetyl-1-pyrroline (2-AP) biosynthesis of fragrant rice, the main fragrant rice variety Meixiangzhan 2 in Guangdong province was used as the material, and a two-year field experiment was carried out. During the heading stage, the fragrant rice plants were sprayed with different concentrations of ornithine solution. Rice quality and 2-AP synthesis characteristics were compared and analyzed. The results showed that spraying ornithine solution with a concentration of 0.4-1.6g/L could significantly increase the chlorophyll content of flag leaves during the filling stage, increase the yield of fragrant rice and the crude protein content of rice, and reduce the chalkiness and chalky grain rate. Compared with the control, the 2-AP contents of fragrant rice under the treatments of spraying ornithine were significantly increased by 14.81%-30.62%. It could be seen that exogenous ornithine has certain application value in fragrant rice production.

Key words: Fragrant rice, Ornithine, 2-acetyl-1-pyrroline, Yield, Rice quality

Table 1

Effects of foliar application of ornithine at heading stage on yield and its components of fragrant rice"

年份
Year		 处理
Treatment		 有效穗数
Productive panicle number (/m2)		 穗粒数
Grains number per panicle		 千粒重
1000-grain weight (g)		 结实率
Seed-setting rate (%)		 产量
Yield (t/hm2)
2019 CK 329.71a 144.22ab 19.90c 72.21c 5.44b
T0.2 328.05a 140.41b 19.75c 72.98c 5.47b
T0.4 329.34a 147.91ab 20.51b 77.47b 5.99a
T0.8 334.33a 146.27ab 21.11a 83.25a 5.98a
T1.6 330.77a 150.50a 20.75ab 82.82a 5.97a
2020 CK 342.05a 149.58a 19.46c 72.07b 5.46b
T0.2 336.36a 144.95a 20.35b 72.95b 5.50b
T0.4 330.05a 144.64a 20.74ab 78.86a 5.61a
T0.8 314.08a 147.70a 20.84a 82.18a 6.04a
T1.6 318.33a 138.01a 21.07a 83.14a 6.16a

Table 2

Effects of foliar application of ornithine at heading stage on grain quality of fragrant rice"

年份
Year		 处理
Treatment		 糙米率
Brown rice rate (%)		 精米率
Milled rice rate (%)		 整精米率
Head rice rate (%)		 垩白粒率
Chalky rice rate (%)		 垩白度
Chalkiness		 粗蛋白
Crude protein (%)		 直链淀粉
Amylose (%)
2019 CK 81.71a 70.65a 58.72a 15.64a 6.26a 8.20c 18.67a
T0.2 80.78ab 70.29a 57.04a 14.46b 6.00a 8.20c 18.59a
T0.4 80.69b 70.40a 58.66a 11.36c 5.23b 8.47b 18.38a
T0.8 80.37b 70.59a 57.72a 11.35c 4.84c 8.77a 18.34a
T1.6 79.82b 71.28a 57.39a 11.56c 4.77c 8.80a 18.64a
2020 CK 80.82a 70.44ab 56.92a 15.42a 6.38a 8.13c 18.27a
T0.2 79.61a 68.77b 57.94a 14.60b 6.18a 8.07c 18.78a
T0.4 80.06a 68.88b 58.56a 11.27c 5.04b 8.47b 18.28a
T0.8 80.02a 70.43ab 59.21a 11.58c 5.13b 8.83a 18.72a
T1.6 80.27a 70.61a 57.98a 11.54c 4.80b 8.70a 18.73a

Fig.1

Effects of foliar application of ornithine at heading stage on chlorophyll content of fragrant rice Different lowercase letters indicate significant difference at the P < 0.05 level, the same below"

Fig.2

Effects of foliar application of ornithine at heading stage on 2-AP content of fragrant rice"

Table 3

Effects of foliar application of ornithine at heading stage on activities of enzymes involved in 2-AP biosynthesis U/(g·min)"

年份
Year		 处理
Treatment		 鸟氨酸转氨酶
Ornithine
aminotransferase		 二胺氧化酶
Diamine
oxidase		 甜菜碱醛脱氢酶
Betaine aldehyde
dehydrogenase
2019 CK 2.07c 3.14c 15.78ab
T0.2 2.23b 3.27c 16.37a
T0.4 2.29b 3.65b 14.96bc
T0.8 2.36b 3.74ab 14.02cd
T1.6 2.53a 3.90a 13.71d
2020 CK 2.08b 3.17c 15.78a
T0.2 2.18b 3.44b 16.25a
T0.4 2.33a 3.64ab 14.46b
T0.8 2.47a 3.86a 13.60b
T1.6 2.38a 3.86a 13.70b
[1] 黄晓琴, 周传猛, 梁琳, 等. 不同香型优质常规稻品种主要农艺性状灰色关联度分析. 中国种业, 2021(9):62-66.
[2] 曾宪楠, 王麒, 宋秋来, 等. 基于Web of Science的香稻研究现状分析. 黑龙江农业科学, 2021(10):130-133.
[3] 潘阳阳, 周德贵, 黄道强, 等. 香稻不同发育时期香味物质的变化分析. 广东农业科学, 2021, 48(10):42-51.
[4] 魏晓东, 张亚东, 赵凌, 等. 稻米香味物质2-乙酰-1-吡咯啉的形成及其影响因素. 中国水稻科学, 2022, 36(2):131-138.
doi: 10.16819/j.1001-7216.2022.201214
[5] 唐湘如, 吴密. 施用锌、铁、镧肥对香稻糙米香气和剑叶脯氨酸含量的影响. 杂交水稻, 2006(6):69-72.
[6] 邢丕鹏, 刘金海, 罗昊文, 等. 有机栽培条件下不同机械种植方式对香稻产量、品质以及香气的影响. 中国稻米, 2021, 27(5):41-44.
doi: 10.3969/j.issn.1006-8082.2021.05.009
[7] 孟庆彬, 黄健文, 冯国辉, 等. 优质三系香稻不育系耕香A的选育. 杂交水稻, 2021, 36(5):14-16.
[8] 宁敏, 刘俊雄, 暴亚冲, 等. 水稻种质资源香味基因Badh2的分子鉴定及香稻筛选. 分子植物育种, 2021, 19(15):5017-5029.
[9] Gholami M, Ghanati F, Fakhari A R, et al. Differential effects of ornithine enantiomers on the activity of anti-oxidant enzymes, polyamines content, and growth of tobacco cells under osmotic stresses. Chirality, 2013, 25(10):583-588.
doi: 10.1002/chir.22173 pmid: 23836605
[10] 夏海威. 外源鸟氨酸和一氧化氮对镉胁迫下菹草缓解效应的研究. 南京:南京师范大学, 2014.
[11] Liu C, Xue Z, Tang D, et al. Ornithine delta-aminotransferase is critical for floret development and seed setting through mediating nitrogen reutilization in rice. Plant Journal, 2018, 96(4):842-854.
doi: 10.1111/tpj.14072
[12] Yoshihashi T, Huong N T T, Inatomi H. Precursors of 2-acetyl- 1-pyrroline, a potent flavor compound of an aromatic rice variety. Journal of Agricultural and Food Chemistry, 2002, 50(7):2001-2004.
pmid: 11902947
[13] 于洋, 敬江博, 王璠. 氨基酸叶面肥在水稻上应用效果. 现代化农业, 2021(7):13-14.
[14] 王艳, 陈芹, 周梅. 氨基酸叶面肥在水稻上应用效果试验总结. 农业开发与装备, 2015(11):72-73.
[15] 余忠平, 李建辉, 张立新. 复旦复农氨基酸叶面肥在水稻上应用效果. 现代化农业, 2010(8):19.
[16] 李国华, 苏娟, 张立新. 恒生氨基酸叶面肥在水稻上应用效果. 现代化农业, 2009(3):15.
[17] 黄忠林, 唐湘如, 王玉良, 等. 增香栽培对香稻香气和产量的影响及其相关生理机制. 中国农业科学, 2012, 45(6):1054-1065.
doi: 10.3864/j.issn.0578-1752.2012.06.003
[18] 赵贵林, 陈强, 胡国霞, 等. 水稻脯氨酸代谢关键酶对水分胁迫的响应. 干旱地区农业研究, 2011, 29(3):80-83.
[19] 李梦兴, 唐湘如, 莫钊文, 等. 硒肥对香稻香气和糙米硒含量以及产量的影响. 华北农学报, 2016, 31(6):213-219.
doi: 10.7668/hbnxb.2016.06.033
[20] 罗笛, 牛向丽, 余进德, 等. 水稻、菠菜甜菜碱醛脱氢酶(BADH)重组蛋白表达及酶活性分析. 农业生物技术学报, 2012, 20(12):1390-1397.
[21] 王奇, 李妹娟, 章家恩, 等. 稻鱼共作对水稻叶绿素荧光特征及产量的影响. 作物杂志, 2021(6):145-151.
[22] 葛君, 刘震. 低温胁迫对拔节期小麦光合色素、光合参数及叶绿素荧光特性的影响. 山西农业科学, 2021, 49(11):1253-1256.
[23] 孟军, 陈温福, 徐正进, 等. 水稻剑叶净光合速率与叶绿素含量的研究初报. 沈阳农业大学学报, 2001(4):247-249.
[24] 朱友理, 何东兵, 邱晓红, 等. 病虫草危害对稻米品质的影响. 中国稻米, 2021, 27(6):115-118.
doi: 10.3969/j.issn.1006-8082.2021.06.023
[25] 田爽, 王晓萍. 水稻蛋白质的研究进展. 哈尔滨师范大学自然科学学报, 2014, 30(5):91-95.
[26] 袁帅, 苏雨婷, 陈平平, 等. 施氮对稻米品质的影响研究进展与展望. 作物研究, 2021, 35(4):394-400.
[27] 殷春渊, 王书玉, 刘贺梅, 等. 水稻食味品质性状间相关性分析及其与叶片光合作用的关系. 中国农业科技导报, 2021, 23(4):119-127.
doi: 10.13304/j.nykjdb.2020.0218
[28] 杨晓龙, 程建平, 汪本福, 等. 灌浆期干旱胁迫对水稻生理性状和产量的影响. 中国水稻科学, 2021, 35(1):38-46.
doi: 10.16819/j.1001-7216.2021.0401
[29] Wakte K, Zanan R, Hinge V, et al. Thirty-three years of 2-acetyl- 1-pyrroline, a principal basmati aroma compound in scented rice (Oryza sativa L.): a status review. Journal of the Science of Food and Agriculture, 2017, 97(2):384-395.
doi: 10.1002/jsfa.2017.97.issue-2
[30] Chen S, Yang Y, Shi W, et al. Badh2,encoding betaine aldehyde dehydrogenase, inhibits the biosynthesis of 2-acetyl-1-pyrroline, a major component in rice fragrance. The Plant Cell, 2008, 20(7):1850-1861.
doi: 10.1105/tpc.108.058917
[31] 李梦兴. 喷施亚硒酸钠对香稻香气和硒含量及产量的影响. 广州:华南农业大学, 2016.
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