Crops ›› 2023, Vol. 39 ›› Issue (4): 104-109.doi: 10.16035/j.issn.1001-7283.2023.04.016

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Analysis of Combining Ability and Genetic Effect of Maize Inbred Lines under Shading Condition

Yuan Liuzheng1,2(), Wang Huiqiang1,2(), WangQiuling 1,2(), Zhu Shidie1,2, ZhaoYueqiang 1,2, Yuan Manman1,2, Wang Huitao1,2, Zhang Yundong1,2, Liu Jiayou1,2(), Yuan Yongqiang1,2   

  1. 1Luohe Academy of Agricultural Sciences,Luohe 462000, Henan, China
    2The Maize Disaster Prevention and Mitigation Engineering Research Center of Henan Province, Luohe 462000, Henan, China
  • Received:2022-02-08 Revised:2022-07-27 Online:2023-08-15 Published:2023-08-15
  • Contact: Liu Jiayou E-mail:yuanliuzheng@126.com;huiqiang715@sina.com;lnkwql@163.com;jiayou.liu@126.com

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Abstract:

In order to improve the tolerance of maize varieties with heterosis,ten inbred lines with different shading tolerance were used as parents, combining ability and genetic parameters of yield and ear characteristics under shading and natural light were analyzed according to NC II 4×6 genetic mating design. The results showed that there were extremely significant differences in yield, ear length, bald tip length, ear diameter, row number per ear, grain number per row and 100-grain weight under natural light and shade treatments. The four inbred lines of Changgai3, R2005, LYM35 and LYM10 had high general combining ability, high special combining ability and strong heterosis under shading treatments, which could be used as candidate parents for shading resistance genetic improvement of maize. The special combining ability effect value of LYM35×Changgai3 was higher in shading treatments, and it showed excellent performance in shading treatments. Genetic parameter analysis showed that the narrow heritabilities of yield, ear length, ear diameter, bald tip length and row number per ear under shading treatments were relatively high, which could be selected for shading tolerance in early generations. The narrow-sense heritabilities of grain number per row and 100-grain weight were low, but the general heritabilities were high, selection should be made in high breeding generations.

Key words: Maize, Heterosis, Tolerance of shade, Combining ability, Narrow-sense heritability, General heritability

Table 1

Inbred lines and origins of test maizes"

编号
Number		 名称
Name		 血缘关系
Kinship
1 昌改3 昌7-2改良系
2 R2005 78599选系
3 LYF9 先锋杂交种选系
4 Y1803 LIBC 4×4CV⊕/4CV
5 LYF5 LH220Ht×6WC⊕/6WC⊕
6 LYM35 PHW17×6WC⊕/6WC/6WC/漯7007/漯7007
7 Y101 R2005×郑58选系
8 LYM90 豫1122×H3659Z
9 LYM11 PHK35×6WC
10 LYM10 德单5号杂交种选系

Table 2

Variance analysis of combing ability on yield and different agronomic traits under different treatments (F value)"

变异来源
Source of
variation		 自由度
Degreeof
freedom		 产量
Yield		 穗长
Ear
length		 穗粗
Ear
diameter		 秃尖长
Bald tip
length		 穗行数
Row number
per ear		 行粒数
Grainnumber
per row		 百粒重
100-grain
weight
CK ZY CK ZY CK ZY CK ZY CK ZY CK ZY CK ZY
区组Group 2 2.28 0.24 2.42 2.64 2.26 0.80 1.51 3.15 0.07 2.84 0.79 1.80 2.11 1.82
组合Combination 23 11.09** 23.33** 89.92** 4.63** 13.25** 3.63** 105.44** 4.84** 8.89** 18.10** 4.17** 24.47** 13.25** 13.20**
亲本1 Parent 1 3 5.15* 5.20* 20.84** 6.49** 4.03* 5.62** 4.47* 8.21** 7.40** 9.06** 3.54* 3.11 29.13** 1.21
亲本2 Parent 2 5 0.79 1.07 1.21 0.97 3.16* 2.49 1.84 1.64 4.24* 0.50 0.37 0.92 1.91 0.81
亲本1×亲本2
Parent 1×parent 2		 15 7.42** 14.92** 24.74** 2.71** 7.10** 1.89 64.47** 2.33* 3.50** 9.32** 3.49** 19.47** 2.72** 13.39**

Table 3

GCA effects of yield and different agronomic traits under different treatments"

自交系
Inbred
line		 产量
Yield		 穗长
Ear length		 穗粗
Ear diameter		 秃尖长
Bald tip length		 穗行数
Row number per ear		 行粒数
Grainnumberper row		 百粒重
100-grainweight
CK ZY CK ZY CK ZY CK ZY CK ZY CK ZY CK ZY
昌改3 Changgai 3 21.28 50.14 -7.15 -10.81 4.62 5.33 -61.81 -45.89 8.83 12.06 5.62 20.60 -7.05 -3.53
R2005 8.73 14.34 -2.35 -2.53 1.66 4.43 0.14 45.83 1.01 4.70 -0.31 -2.75 -4.90 3.87
LYF9 -27.01 -30.59 -8.08 -8.75 -3.56 -2.30 -9.48 -15.58 -0.87 -5.06 -9.57 -10.66 -2.11 -2.48
Y1803 -3.00 -33.88 17.57 22.09 -2.71 -7.46 71.15 15.63 -8.98 -11.69 4.26 -7.19 14.06 2.13
LYF5 -1.84 -20.00 5.87 6.19 -2.91 -6.01 -32.11 20.21 -7.99 -2.40 1.41 -11.29 5.10 1.00
LYM35 1.36 18.72 1.43 6.64 -2.31 0.58 1.84 2.24 -2.80 -0.63 3.45 -8.23 0.69 -5.67
Y101 3.89 -5.21 0.92 -10.10 -4.83 -4.35 -66.05 -39.24 -6.51 -3.15 0.67 -5.30 -4.19 -1.15
LYM90 -18.28 -25.84 -2.67 -5.57 6.43 7.91 23.06 -6.03 8.34 4.41 -2.66 9.08 -0.58 3.71
LYM11 10.57 0.27 -3.95 5.76 2.68 1.62 54.46 4.42 6.26 2.98 -3.81 5.69 -0.11 3.65
LYM10 4.30 32.05 -1.59 -2.92 0.94 0.25 18.81 18.41 2.70 -1.21 0.94 10.06 -0.92 -1.54

Table 4

SCA effects of yield and different agronomic traits under different treatments"

组合
Combination		 产量
Yield		 穗长
Ear length		 穗粗
Ear diameter		 秃尖长
Bald tip length		 穗行数
Row number per ear		 行粒数
Grainnumberper row		 百粒重
100-grainweight
CK ZY CK ZY CK ZY CK ZY CK ZY CK ZY CK ZY
LYF5×昌改3
LYF5×Changgai 3		 -3.21 -40.27 -0.62 -5.14 -1.92 -0.78 -6.08 -37.74 -3.49 -3.43 -1.28 -0.82 1.07 2.96
LYM35×昌改3
LYM35×Changgai 3		 5.59 62.74 0.43 -1.77 0.24 -3.58 27.86 8.97 -7.49 -2.70 4.01 7.80 -0.48 6.94
Y101×昌改3
Y101×Changgai 3		 16.44 -11.96 6.38 18.10 1.47 1.35 27.86 28.25 2.15 4.81 9.23 -1.23 2.74 -5.29
LYM90×昌改3
LYM90×Changgai 3		 -17.05 -73.88 -4.92 -24.05 3.40 -2.57 -27.30 -28.48 3.93 -0.50 -5.07 -14.32 4.87 1.14
LYM11×昌改3
LYM11×Changgai 3		 -15.35 50.50 -4.00 2.56 -4.75 -1.59 -16.27 23.13 3.64 1.19 -7.17 -0.40 -8.81 -9.06
LYM10×昌改3
LYM10×Changgai 3		 13.57 12.88 2.73 10.30 1.56 7.17 -6.08 5.87 1.26 0.63 0.28 8.97 0.61 3.31
LYF5×R2005 10.71 37.90 9.73 9.84 2.14 2.58 -0.14 22.11 -0.42 2.18 4.37 28.86 3.32 0.86
LYM35×R2005 27.42 1.37 -1.24 -0.60 2.65 11.22 33.80 -12.19 2.10 -2.01 4.51 10.55 2.59 5.64
Y101×R2005 -23.67 2.65 -0.22 2.30 -1.43 1.31 -0.14 -3.37 2.84 0.93 -3.02 -8.63 -2.52 9.86
LYM90×R2005 28.79 44.47 4.91 4.29 3.61 -5.26 -38.33 16.99 3.44 3.11 8.98 -1.42 -6.82 -0.70
LYM11×R2005 -30.08 -67.12 -12.34 -19.41 -3.26 -6.56 -45.97 16.56 -7.54 -3.20 -11.02 -33.05 1.56 -12.34
LYM10×R2005 -13.17 -19.27 -0.84 3.58 -3.72 -3.29 50.78 -40.11 -0.42 -1.01 -3.83 3.70 1.87 -3.32
LYF5×LYF9 -1.02 -0.46 -7.39 -4.64 -1.98 2.68 -58.42 -26.23 3.83 8.44 -1.55 -12.82 -5.50 0.38
LYM35×LYF9 -12.14 -31.14 0.65 2.68 0.54 -4.16 -41.44 24.40 -2.55 3.67 -3.04 -2.07 1.83 0.04
Y101×LYF9 -12.76 24.20 -3.99 5.06 -3.72 -3.34 -7.50 -13.82 -8.34 -10.56 -9.75 15.53 -1.77 -4.20
LYM90×LYF9 -17.05 -2.65 -0.65 -3.54 -7.65 -1.06 157.99 47.92 -5.37 -14.87 -10.22 -18.31 2.76 -3.72
LYM11×LYF9 28.58 26.76 7.72 2.37 4.89 1.31 7.78 -50.29 1.46 -0.43 10.99 14.07 4.67 11.05
LYM10×LYF9 14.39 -16.71 3.66 -1.93 7.91 4.57 -58.42 18.03 10.96 13.75 13.57 3.60 -1.99 -3.54
LYF5×Y1803 -6.48 2.83 -1.72 -0.05 1.75 -4.48 64.64 41.86 0.07 -7.18 -1.55 -15.22 1.11 -4.20
LYM35×Y1803 -20.87 -32.97 0.16 -0.32 -3.43 -3.48 -20.23 -21.19 7.94 1.04 -5.48 -16.29 -3.95 -12.62
Y101×Y1803 19.99 -14.89 -2.17 -25.46 3.67 0.68 -20.23 -11.06 3.34 4.81 3.54 -5.66 1.55 -0.37
LYM90×Y1803 5.32 32.05 0.66 23.30 0.65 8.90 -92.36 -36.43 -2.00 12.25 6.32 34.05 -0.81 3.29
LYM11×Y1803 16.85 -10.14 8.62 14.48 3.12 6.84 54.46 10.61 2.45 2.44 7.20 19.38 2.58 10.35
LYM10×Y1803 -14.80 23.11 -5.56 -11.95 -5.76 -8.46 13.72 16.21 -11.80 -13.37 -10.02 -16.27 -0.49 3.55

Table 5

Genetic parameters of main traits under different treatments"

项目
Item		 产量
Yield		 穗长
Ear
length		 穗粗
Ear
diameter		 秃尖长
Bald tip
length		 穗行数
Row number
per ear		 行粒数
Grainnumber
per row		 百粒重
100-grain
weight
CK ZY CK ZY CK ZY CK ZY CK ZY CK ZY CK ZY
加性方差
Additive variance		 12704.54 6729.55 3.60 3.43 0.02 0.06 0.06 0.22 0.91 1.74 3.20 6.45 7.57 0.22
非加性方差
Non-additive variance		 15905.85 8971.07 1.04 2.36 0.04 0.04 0.10 0.11 0.61 1.16 5.39 17.38 1.02 5.78
环境方差
Environmental variance		 7434.10 1932.97 0.13 4.14 0.02 0.12 0.01 0.24 0.73 0.42 6.48 2.82 1.78 1.40
一般配合力方差
Variance of GCA (%)		 44.41 43.47 77.78 59.19 54.90 70.89 44.48 70.47 72.45 60.07 37.20 27.05 88.60 3.59
特殊配合力方差
Variance of SCA (%)		 55.59 56.53 22.22 40.81 45.10 29.11 55.52 29.53 27.55 39.93 62.80 72.95 11.40 96.41
广义遗传力
General heritability (%)		 79.38 89.14 97.27 58.29 81.85 50.30 97.44 60.00 75.14 87.41 56.97 89.41 83.44 81.07
狭义遗传力
Narrow-sense heritability (%)		 35.25 38.75 75.66 34.50 44.93 35.66 43.34 42.28 54.44 52.51 21.20 24.18 73.92 2.91
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