作物杂志,2016, 第1期: 6268 doi: 10.16035/j.issn.1001-7283.2016.01.012
刘新颖1,王柏凤2,周琳1,冯树丹1,宋新元2
Liu Xinying1,Wang Baifeng2,Zhou Lin1,Feng Shudan1,Song Xinyuan2
摘要:
选取我国具有自主知识产权的转cry1Ie基因抗虫玉米IE09S034,利用手捡法和巴氏罐诱捕法,以群落组成、群落结构、优势类群种群动态为参数,研究转基因玉米IE09S034对田间大型土壤动物多样性的影响。研究结果表明,转基因玉米IE09S034较非转基因对照综31,对田间大型土壤动物的群落组成、物种丰富度、Shannon-Wiener多样性指数、Pielou均匀度指数以及优势类群种群动态等均无显著影响,可初步认定转基因抗虫玉米IE09S034对田间大型土壤动物无安全性风险。
[1] | 数据基因农业网.ISAAA信息.生物技术通报, 2015(2):102. |
[2] | 刘慧, 何康来, 白树雄 , 等. 转cry1Ab基因玉米对瓢虫科天敌种群动态的影响. 生物安全学报, 2012,21(2):130-134. |
[3] | 刘俊峰, 邸宏, 曾兴 , 等. 转BcBCP1基因耐盐碱玉米对田间节肢动物群落的影响分析.玉米科学, 2013(3):35-39. |
[4] | Stephens E J, Losey J E, Allee L L , et al. The impact of Cry3Bb Bt-maize on two guilds of beneficial beetles.Agriculture, Ecosystems & Environment, 2012,156:72-81. |
[5] | 孙彩霞, 武志杰, 陈利军 , 等. 转Bt基因玉米的生态安全性研究进展. 生态学报, 2004,24(4):798-805. |
[6] | 吴立成, 李啸风, 叶庆富 , 等. 转cry1Ab基因水稻中毒蛋白的表达、分泌及其在土壤中的残留. 环境科学, 2004,25(5):116-121. |
[7] | Icoz I, Stotzky G . Fate and effects of insect-resistant Bt crops in soil ecosystems. Soil Biology & Biochemistry, 2008,40(3):559-586. |
[8] | 杨艳, 李云河, 曹凤勤 , 等. 转Bt基因抗虫作物对鳞翅目非靶标昆虫生态影响的研究进展. 生物安全学报, 2015,23(4):224-237. |
[9] | 袁一杨, 戈峰 . 转Bt基因作物对非靶标土壤动物的影响.应用生态学报, 2010(5):1339-1345. |
[10] | 尹文英 . 中国土壤动物检索图鉴.北京: 科学出版社, 1998. |
[11] | 钟觉民 . 幼虫分类学.北京: 农业出版社, 1990. |
[12] | 张巍巍, 李元胜 . 中国昆虫生态大图鉴.重庆: 重庆大学出版社, 2011. |
[13] |
Yuan Y, Xin K, Chen F , et al. Decrease in catalase activity of Folsomia candida fed a Bt rice diet. Environmental Pollution, 2011,159(12):3714-3720.
doi: 10.1016/j.envpol.2011.07.015 |
[14] |
Cortet J, Griffiths B S, Bohanec M , et al. Evaluation of effects of transgenic Bt maize on microarthropods in a European multi-site experiment. Pedobiologia, 2007,51(3):207-218.
doi: 10.1016/j.pedobi.2007.04.001 |
[15] |
Vaufleury A D, Kramarz P E, Binet P , et al. Exposure and effects assessments of Bt-maize on non-target organisms (gastropods, microarthropods,mycorrhizal fungi) in microcosms. Pedobiologia, 2007,51(3):185-194.
doi: 10.1016/j.pedobi.2007.04.005 |
[16] |
Birch A N E, Griffiths B S, Caul S , et al. The role of laboratory,glasshouse and field scale experiments in understanding the interactions between genetically modified crops and soil ecosystems:A review of the ECOGEN project. Pedobiologia, 2007,51(3):251-260.
doi: 10.1016/j.pedobi.2007.04.008 |
[17] |
Bing Y, Hui C, Liu X , et al. Bt cotton planting does not affect the community characteristics of rhizosphere soil nematodes. Applied Soil Ecology, 2014,73(1):156-164.
doi: 10.1016/j.apsoil.2013.09.001 |
[18] | Saxena D, Stotzky G . Bacillus thuringiensis (Bt) toxin released from root exudates and biomass of Bt corn has no apparent effect on earthworms,nematodes,protozoa,bacteria,and fungi in soil. Soil Biology & Biochemistry, 2001,33(1):1225-1230. |
[19] | Zeilinger A R, Andow D A, Zwahlen C , et al. Earthworm populations in a northern U.S.Cornbelt soil are not affected by long-term cultivation of Bt maize expressing Cry1Ab and Cry3Bb1 proteins. Soil Biology & Biochemistry, 2010,42(8):1284-1292. |
[20] |
Weber M, Nentwig W . Impact of Bt corn on the diplopod Allajulus latestriatus. Pedobiologia, 2006,50(4):357-368.
doi: 10.1016/j.pedobi.2006.06.004 |
[21] |
Floate K D, Cárcamo H A, Blackshaw R E , et al. Response of ground beetle (Coleoptera:Carabidae) field populations to four years of Lepidoptera-specific Bt corn production. Environmental Entomology, 2007,36(5):1269-1274.
doi: 10.1603/0046-225X(2007)36[1269:ROGBCC]2.0.CO;2 |
[22] | Manachini B . Ground beetle assemblages (Coleoptera,Carabidae) and plant dwelling non-target arthropods in isogenic and transgenic corn crops. Bollettino Di Zoologia Agraria E Di Bachicoltura, 2000,32:181-198. |
[23] | Twardowski J, Beres P, Hurej M , et al. A quantitative assessment of the unintended effects of Bt-maize (MON 810) on rove beetle (Coleoptera,Staphylinidae) assemblages. Polish Journal of Environmental Studies, 2014,23(1):215-220. |
[24] |
祝向钰, 李志毅, 常亮 , 等. 转Bt水稻土壤跳虫群落组成及其数量变化. 生态学报, 2012,32(11):3546-3554.
doi: 10.5846/stxb201105170640 |
[25] |
Chang L, Liu X, Ge F . Effect of elevated O3 associated with Bt cotton on the abundance,diversity and community structure of soil Collembola. Applied Soil Ecology, 2011,47(1):45-50.
doi: 10.1016/j.apsoil.2010.10.013 |
[26] | 郭井菲, 张聪, 袁志华 , 等. 转cry1Ie基因抗虫玉米对田间节肢动物群落多样性的影响. 植物保护学报, 2014,41(4):482-489. |
[27] | 王培, 王应伦, 王振营 , 等. 转cry1Ac基因玉米对玉米田非靶标节肢动物群落多样性的影响.植保科技创新与病虫防控专业化——中国植物保护学会2011年学术年会论文集.北京: 中国农业科学技术出版社, 2011: 814. |
[1] | 李丽娜,金龙国,谢传晓,刘昌林. 转基因玉米和转基因大豆盲样检测方法[J]. 作物杂志, 2017, (6): 3744 |
[2] | 梁晋刚,张正光. 转基因作物种植对土壤生态系统影响的研究进展[J]. 作物杂志, 2017, (4): 16 |
[3] | 郭翠,张维,余桂容,周正富,李亮,冯帅,陈明,王劲. 转G2-EPSPS基因玉米D-3侧翼序列分析与转化体特异性检测方法[J]. 作物杂志, 2016, (1): 6975 |
[4] | 韩猛, 王霄汉, 张中保, 等. BADH基因转人玉米自交系的研究[J]. 作物杂志, 2015, (2): 6469 |
[5] | 李杰, 郭欣慰, 张中保, 等. 将拟南芥ATNCED3基因导人玉米自交系的研究[J]. 作物杂志, 2014, (1): 5862 |
[6] | 赫福霞, 郎志宏, 张杰, 陆伟, 何康来, 黄大昉. 转Bt cry1Ah/2mG2-epsps双价基因抗虫/耐除草剂玉米研究[J]. 作物杂志, 2012, (6): 2933 |
[7] | 陈丽娟, 黄从林, 张秀海, 等. CspB基因植物表达载体的构建及转化玉米自交系的研究[J]. 作物杂志, 2011, (3): 7074 |
[8] | 杨朝国, 杨方方, 张登峰. 抗除草剂草丁膦转基因玉米后代筛选方法的研究[J]. 作物杂志, 2010, (6): 3235 |
|