作物杂志,2018, 第6期: 68–75 doi: 10.16035/j.issn.1001-7283.2018.06.011

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

一株甘蔗内生菌鉴定及其溶磷能力的研究

狄义宁1,刘鲁峰1,谢林艳1,李咏梅2,何鹏飞2,崔文艳2,李富生1,何丽莲1,3   

  1. 1 云南农业大学农学与生物技术学院,650201,云南昆明
    2 云南农业大学植物保护学院,650201,云南昆明
    3 云南农业大学甘蔗研究所,650201,云南昆明
  • 收稿日期:2018-06-22 修回日期:2018-09-13 出版日期:2018-12-15 发布日期:2018-12-06
  • 作者简介:狄义宁,硕士研究生,研究方向为植物资源评价与利用
  • 基金资助:
    云南省现代农业甘蔗产业技术体系建设专项(2017-2018);福建农林大学国家甘蔗工程技术研究中心开放课题(NER02018.6.1)

Identification and Characterization of a Phosphate-Solubilizing Endophyte from Sugarcane

Di Yining1,Liu Lufeng1,Xie Linyan1,Li Yongmei2,He Pengfei2,Cui Wenyan2,Li Fusheng1,He Lilian1,3   

  1. 1 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan,China
    2 College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan, China
    3 Sugarcane Research Institute, Yunnan Agricultural University, Kunming 650201, Yunnan, China
  • Received:2018-06-22 Revised:2018-09-13 Online:2018-12-15 Published:2018-12-06

摘要:

采用形态观察、生理生化测定以及16S rDNA、gyrB和rpoB基因片段比对,对分离自甘蔗品种粤糖86-368茎部内的一株D5内生菌进行分类鉴定。在此基础上,使用平板溶磷圈法和液体培养法研究其溶磷能力,并通过盆栽试验探究其对玉米幼苗生长的影响。结果表明:D5菌株属于Pseudomonas extremorientalis;D5菌株对不同磷酸盐的溶磷能力表现不同,表现为磷酸三钙>植酸钙>磷酸铁,溶磷量分别为0.939±0.012、0.655±0.016、0.125±0.005mg/mL,同时,在溶磷过程中,液体培养基的pH均有不同程度的降低,活菌数除了在磷酸铁培养基中一直下降外,其他磷源培养基中均是先升后降的趋势。施用菌剂后,处理组(D5+P)比对照组(CK+P)玉米幼苗的总鲜重、总干重、苗株总磷含量分别增加45.92%、32.65%、45.01%。因此,D5菌株具有制备微生物菌肥的潜力。

关键词: 甘蔗, 内生菌, 分子鉴定, 溶磷

Abstract:

An endophytic D5 strain was isolated from the stem of Yuetang 86-368 which came from one of the sugarcane cultivars.Colony morphology characteristics, physiological and biochemical tests, 16S rDNA, gyrB and rpoB sequence analyses were carried out. Phosphate-solubilizing capability of the strain was studied by methods of phosphorus hole and liquid culture, and the effect on the growth of maize seedling was studied by a pot experiment. The results showed that the D5 strain belonged to Pseudomonas extremorientalis. Its phosphate-solubilizing capability to different phosphate was different which followed the trend: tricalcium phosphate > phytic acid calcium > iron phosphate. The phosphate-solubilizing capacity was 0.939±0.012, 0.655±0.016, 0.125±0.005mg/mL, respectively. The pH value of the liquid media decreased to different extents and the numbers of living bacterium followed a trend of initial rise and then fall in all phosphorus source in the culture medium except in the iron phosphate cultivation showing a straight decline of dissolved phosphorus. Compared with the control (CK+P), the total fresh weight, total dry weight and total phosphorus content of maize seedlings in the D5 strain were increased by 45.92%, 32.65% and 45.01%, respectively. Therefore, it is an excellent candidate for microbial fertilizer.

Key words: Sugarcane, Endophyte, Molecular identification, Phosphate solubilizing

表1

平板溶磷圈法测量的菌