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作物杂志,2020, 第6期: 163–169 doi: 10.16035/j.issn.1001-7283.2020.06.024

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

链霉菌株Streptomyces sp. FXP04对水稻种子萌发和幼苗生长的影响

付学鹏1,2(), 沈童飞1, 孙晓波3, 刘晓涵1, 杨晓杰1,2()   

  1. 1齐齐哈尔大学生命科学与农林学院,161006,黑龙江齐齐哈尔
    2黑龙江省抗性基因工程与寒地生物多样性保护重点实验室,161006,黑龙江齐齐哈尔
    3泰来县农业技术推广中心,162400,黑龙江泰来
  • 收稿日期:2020-05-02 修回日期:2020-10-14 出版日期:2020-12-15 发布日期:2020-12-09
  • 通讯作者: 杨晓杰
  • 作者简介:付学鹏,研究方向为作物病虫害生物防控,E-mail： 02383@qqhru.edu.cn
  • 基金资助:
    黑龙江省自然科学基金(C2017068);黑龙江省教育厅基本业务专项(135109254);植物性食品加工技术特色学科专项(YSTSXK201888)

Effects of Streptomyces sp. FXP04 on Seed Germination and Seedling Growth of Rice

Fu Xuepeng1,2(), Shen Tongfei1, Sun Xiaobo3, Liu Xiaohan1, Yang Xiaojie1,2()   

  1. 1Department of Life Sciences and Agroforestry, Qiqihar University, Qiqihar 161006, Heilongjiang, China
    2Heilongjiang Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, Qiqihar 161006, Heilongjiang, China
    3Agricultural Technology Extension Center of Tailai County, Tailai 162400, Heilongjiang, China
  • Received:2020-05-02 Revised:2020-10-14 Online:2020-12-15 Published:2020-12-09
  • Contact: Yang Xiaojie

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摘要：

为了明确链霉菌株Streptomyces sp. FXP04对水稻的促生效果,以前期筛选到的链霉菌株Streptomyces sp. FXP04为供试菌株,采用发酵液浸种和菌液喷洒土壤的方法,研究了该菌株生长稳定期的发酵液和菌液对水稻种子萌发和幼苗生长的影响。结果如下,培养皿试验中,Streptomyces sp. FXP04发酵液原液和LB培养液原液（对照）都抑制种子萌发,1/50倍发酵液显著促进种子萌发,而1/10倍和1/100倍发酵液对种子萌发没有显著影响。盆栽试验中,1/50倍和1/100倍的Streptomyces sp. FXP04菌液都促进了水稻种子萌发,增加了水稻幼苗株高、主根长、干重、根总长度、须根数、根表面积和根总体积,并且1/50倍菌液的促生效果和1/100倍菌液无显著差异。研究结果表明,链霉菌株Streptomyces sp. FXP04对水稻种子萌发和幼苗生长有促进作用,可作为水稻的候选促生菌株开展后续研究和应用。

关键词: 链霉菌, Streptomyces sp. FXP04, 水稻, 种子萌发, 幼苗生长

Abstract:

In order to determine the growth promoting effects of Streptomyces sp. FXP0404 on rice growth, the Streptomyces sp. FXP04 isolated in our previous experiments was selected as the test strain to study the effects of fermented liquid and cell suspension of Streptomyces sp. FXP04 on the seed germination and seedling growth of rice, using the methods of soaking seeds with fermented liquid and spraying soil with cell suspension. The results showed that both the original fermented liquid and LB liquid (control) inhibited seeds germination in the culture dish. Compared to water treatment, the rice seed germination was significantly increased in the treatment of 1/50 times fermented liquid, however, there were no significant effect on seed germination in the 1/10 and 1/100 times fermented liquid treatments. In the pot experiment, both 1/50 and 1/100 times cell suspension of Streptomyces sp. FXP04 promoted the germination of rice seeds, increased the plant height, main root length, dry weight, total root length, fibrous root number, root surface area and total root volume of rice seedlings, however, there was no significant difference between 1/50 and 1/100 times of Streptomyces sp. FXP04 cell suspension. The results demonstrated that Streptomyces sp. FXP04 could promote the seed germination and seedling growth of rice, and could be used as a candidate plant growth-promoting rhizobacteria on rice for further research and application.

Key words: Streptomyces, Streptomyces sp. FXP04, Rice, Seed germination, Seedling growth

表1

发酵液对水稻种子萌发率的影响

处理Treatment 处理后天数Days after treating (d)
6 8 10
LB培养液原液Original LB liquid 0.00±0.00d 0.00±0.00c 0.00±0.00d
发酵液原液Original fermentation liquid 0.00±0.00d 0.00±0.00c 0.00±0.00d
1/10倍LB培养液1/10 times LB liquid 62.60±4.42c 83.34±5.21b 86.66±5.64c
1/10倍发酵液1/10 times fermentation liquid 74.00±6.32a 89.34±4.58b 93.34±8.54b
1/50倍LB培养液1/50 times LB liquid 68.00±5.20b 86.66±6.35b 88.46±6.52bc
1/50倍发酵液1/50 times fermentation liquid 78.66±4.33a 94.00±8.25a 96.60±8.25a
1/100倍LB培养液1/100 times LB liquid 69.34±5.24b 85.34±7.53b 89.44±7.97bc
1/100倍发酵液1/100 times fermentation liquid 72.66±5.27b 90.00±8.25ab 90.00±8.25c
H2O 72.00±6.33b 92.00±9.62a 93.34±8.54b

图1

发酵液对水稻种子萌发的影响

表2

土培条件下菌液对水稻种子发芽率的影响

处理Treatment 处理后天数
Days after treating (d)
7 18
1/50倍菌液1/50 times cell suspension 20.00±2.00ab 79.00±3.00a
1/50倍LB培养液1/50 times LB liquid 12.00±1.00b 51.33±8.02c
1/100倍菌液1/100 times cell suspension 26.67±3.51a 76.33±4.51a
1/100倍LB培养液1/100 times LB liquid 25.67±5.03a 66.00±2.65b

表3

土培条件下发酵液对水稻幼苗生长的影响

处理Treatment 株高
Plant height (cm)		 主根长
Main root length (cm)
1/50倍菌液
1/50 times cell suspension		 15.88±1.36a 13.88±1.26a
1/50倍LB培养液
1/50 times LB liquid		 12.50±0.64b 9.325±1.00b
1/100倍菌液
1/100 times cell suspension		 15.20±0.87a 12.50±1.66a
1/100倍LB培养液
1/100 times LB liquid		 13.20±1.30b 8.60±0.99b

图2

土培条件下菌液对水稻幼苗生长的影响

图3

菌液对水稻根系生长的影响 CP50和CP100分别表示1/50和1/100倍的菌液;LB50和LB100分别表示1/50和1/100倍的LB培养液;柱上方的不同小写字母表示组间差异显著（P<0.05）,下同。D表示根直径（mm）

图4

菌液对水稻幼苗干重和根冠比的影响

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