作物杂志,2013, 第3期: 53–55

• 论文 • 上一篇    下一篇

渗透胁迫和盐胁迫对荞麦硝酸还原酶及亚硝酸还原酶活性的影响

杨洪兵,   

  • 出版日期:2013-06-15 发布日期:2018-08-25

  • Online:2013-06-15 Published:2018-08-25

摘要: 以盐敏感荞麦品种TQ-0808和耐盐荞麦品种川荞1号为试验材料,采用NaCl和等渗PEG-6000处理,研究渗透胁迫和盐胁迫对不同耐盐性荞麦品种硝酸还原酶(NR)及亚硝酸还原酶(NiR)活性的影响。结果表明,高浓度盐胁迫下盐敏感荞麦品种叶片NR及NiR活性显著降低,而耐盐荞麦品种降低幅度相对较小,且高浓度盐胁迫下盐敏感荞麦品种叶片NR及NiR活性的降低幅度明显大于渗透胁迫的,说明Na+毒害效应发挥了主要作用。另外,两个荞麦品种叶片NR活性高低与其叶片硝酸盐含量呈正相关。

关键词: 荞麦, 渗透胁迫, 盐胁迫, 硝酸还原酶, 亚硝酸还原酶

Abstract: The salt-sensitive buckwheat variety(TQ-0808) and salt-tolerant buckwheat variety(Chuanqiao No.1) were used as experimental materials,which were treated with NaCl and iso-osmotic PEG-6000.The effects of osmotic and salt stress on nitrate reductase and nitrite reductase activity of buckwheat varieties with different salt tolerance were studied.The results showed that the nitrate reductase and nitrite reductase activity of leaves in salt-sensitive buckwheat variety were decreased significantly under the salt stress of high concentration,while that in salt-tolerant buckwheat variety were decreased relatively small,and that in salt-sensitive buckwheat variety under the salt stress of high concentration were obviously more than those under the osmotic stress.It indicated that ion toxicity effects of Na+ play the major role.In addition,the level of nitrate reductase activity of leaves in two buckwheat varieties was positively correlated with the nitrate content of leaves.

Key words: Buckwheat;Osmotic stress;Salt stress;Nitrate reductase;Nitrite reductase

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