致病疫霉拮抗细菌SR13-2菌株的鉴定及对马铃薯离体组织的防病作用

doi:10.16035/j.issn.1001-7283.2017.03.026

摘要/Abstract

摘要：

SR13-2菌株是前期从种植莴苣的土壤中分离获得的对致病疫霉菌丝生长和孢子萌发有强烈抑制作用的拮抗细菌,为进一步了解其生物学特性以及防治马铃薯晚疫病的潜力,本试验依据其培养特征、生理生化特性及16S rDNA序列对该菌株进行了鉴定,并测试了其菌液在离体马铃薯组织上的防病效果。结果显示该菌株属于深红沙雷氏菌(Serratia rubidaea),菌液在马铃薯块茎切片上对晚疫病的预防效果可达91.83%,治疗效果可达83.33%,显著优于甲霜灵锰锌以及其他几株拮抗细菌,在马铃薯离体叶片上也获得了相似的结果。这表明SR13-2菌株在马铃薯晚疫病防治方面有较大潜力。

关键词: 拮抗细菌, 致病疫霉, 鉴定, 马铃薯, 病害防治

Abstract:

Bacterial SR13-2 strain which strongly inhibited mycelial growth and spore germination of Phytophthora infestans was obtained in earlier experiment in our lab. In order to further understand its biological characteristics and potential in controlling potato late blight, the taxonomic status of this stain was identified, and control efficiency on potato tuber slices and detached leaves were detected in this experiment. The results showed that SR13-2 strain was identified as Serratia rubidaea based on cultural features, thallus morphology, gram staining, spore staining, physiological and biochemical characteristics, as well as 16S rDNA sequences. The prevention efficiency and therapy efficiency of bacterial fluid of this strain on potato tuber slices were 91.83% and 83.33%, respectively, which were significantly better than treatments of Ridomil-MZ and other antagonistic bacteria. Similar results were also obtained on detached potato leaves. These results indicated that SR13-2 strain has a great potential in controlling potato late blight in future.

Key words: Antanistic bacteria, Phytophthora infestans, Identification, Potato, Disease control

蒋继志,王游游,王雪宁,李丽艳,万安琪,李明. 致病疫霉拮抗细菌SR13-2菌株的鉴定及对马铃薯离体组织的防病作用[J]. 作物杂志, 2017 (3): 146–150

Jizhi Jiang,Youyou Wang,Xuening Wang,Liyan Li,Anqi Wan,Ming Li. Identification of SR13-2 Strain against Phytophthora infestans and Control of Late Blight on Detached Potato Tissues[J]. Crops, 2017(3): 146–150

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特性Character	SR13-2	Serratia rubidaea	Serratia nematodiphila	Serratia marcescens
精氨酸双水解酶Arginine dihydrolase	-	-	-	-
鸟氨酸脱羧酶Ornithine decarboxylase	-	-	-	+
赖氨酸脱羧酶Lysine decarboxylase	-	-	-	+
葡萄糖产气Glucose gas production	+	+	-	-
葡萄糖产酸Glucose acid production	-	-	+	+
阿拉伯糖产酸Arabinose acid production	+	+	-	-
阿东醇产酸Adonitol acid production	+	+	-	-
卫矛醇产酸Dulcitol acid production	-	-	-	-
乳糖产酸Lactose acid production	+	+	-	-
利用棉子糖Raffinose utilization	+	+	-	-
利用鸟氨酸Ornithine utilization	+	+	-	+
利用阿拉伯糖Arabinose utilization	-	-	-	+
利用木糖Xylose utilization	+	+	-	+
DNA酶DNA enzyme	+	+	+	+
4℃生长Growth at 4℃	-	-	-	+

菌株 Strain	病害预防Disease prevention		病害治疗Disease therapy
菌株 Strain	病情指数 Disease index	效果(%) Efficiency	病情指数 Disease index	效果(%) Efficiency
SR13-2	8.17d	91.83	16.67f	83.33
S34-1	10.00cd	90.00	50.00e	50.00
SR13-1	10.00cd	90.00	91.67b	8.33
SH-9	13.10c	86.90	75.00d	25.00
YHX3	15.00c	85.00	55.00e	45.00
HT-6	16.16c	83.84	85.31c	14.69
Control-1	60.00b	40.00	82.35c	17.65
Control-2	100.00a	0.00	100.00a	0.00

菌株 Strain	病害预防Disease prevention		病害治疗Disease therapy
菌株 Strain	病情指数 Disease index	效果(%) Efficiency	病情指数 Disease index	效果(%) Efficiency
SR13-2	7.13e	92.87	14.73f	85.27
S34-1	13.60d	86.40	45.30e	54.70
SR13-1	13.30cd	86.70	87.74b	12.26
SH-9	14.52cd	85.48	66.20d	33.80
YHX3	19.70c	80.30	63.71d	36.29
HT-6	20.15c	79.85	80.00c	20.00
Control-1	58.30b	41.70	83.50de	16.50
Control-2	100.00a	0.00	100.00a	0.00