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作物杂志,2019, 第6期: 14–19 doi: 10.16035/j.issn.1001-7283.2019.06.003

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

黄淮南片麦区新育成品种（系）中3个矮秆基因分子标记检测及其与农艺性状的关系

曹廷杰1,张玉娥1,胡卫国1,杨剑1,赵虹1,王西成1,周艳杰1,赵群友2,李会群3   

  1. 1河南省农业科学院小麦研究所,450002,河南郑州
    2南阳市种子管理站,473000,河南南阳
    3濮阳市种子管理站,457000,河南濮阳
  • 收稿日期:2019-05-28 修回日期:2019-08-21 出版日期:2019-12-15 发布日期:2019-12-11
  • 作者简介:曹廷杰，副研究员，主要从事小麦育种及重要性状遗传分析研究
  • 基金资助:
    河南省农业科学院自主创新基金,河南省小麦产业技术体系(S2010-01-G03)

Detection of Three Dwarfing Genes in the New Wheat Cultivars (Lines) Developed in South Huang-Huai Valley and Its Association with Agronomic Traits

Cao Tingjie1,Zhang Yu’e1,Hu Weiguo1,Yang Jian1,Zhao Hong1,Wang Xicheng1,Zhou Yanjie1,Zhao Qunyou2,Li Huiqun3   

  1. 1Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
    2Seed Management Department of Nanyang, Nanyang 473000, Henan, China
    3Seed Management Department of Puyang, Puyang 457000, Henan, China
  • Received:2019-05-28 Revised:2019-08-21 Online:2019-12-15 Published:2019-12-11

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摘要：

利用矮秆基因RhtB1-b、RhtD1-b和Rht8特异分子标记对郑麦583和2015-2016年度参加河南省区域试验、河南省品种比较试验、国家黄淮南片区域试验及国家黄淮麦区品种比较试验的共630份小麦材料的基因型进行检测。结果表明,供试材料中检测到549份材料含有RhtB1-b基因;592份材料含有RhtD1-b基因;513份材料含有Rht8基因;422份材料同时含有3个矮秆基因,169份材料仅含有2个矮秆基因,说明3个主要的矮秆基因在河南小麦育种过程中被聚合使用。此外,分析发现,矮秆基因Rht8与株高和每公顷穗数,以及千粒重具有显著相关性。郑麦583等小麦品种聚合了这3个矮秆基因,具有优良的丰产性,通过选择和利用矮秆基因对于培育具有丰产性优点的小麦品种具有一定价值。

关键词: 小麦, 株高, 矮秆基因, 农艺性状

Abstract:

In this study, Zhengmai 583 and 630 materials derived from variety comparison test and regional trials of Yellow-Huai wheat area and Henan Province at 2015-2016 were screened by specific molecular marker of wheat dwarf genes RhtB1-b, RhtD1-b and Rht8. The results showed that 549 wheat materials carried RhtB1-b gene; 592 wheat materials carried RhtD1-b gene; 513 wheat materials carried Rht8 gene; 422 materials carried all 3 dwarfing genes, and additional 169 lines carried 2 dwarfing genes which indicated that the 3 dwarf genes had been fixed in Henan wheat breeding progress. Moreover, Rht8 showed significantly correlation with reducing plant height, spike number per hectare and increasing 1000-kernel weight. Zhengmai 583 carried 3 dwarf genes, showed excellent fertility. The selection and utilization of dwarf genes are of certain value for the cultivation of wheat varieties with high yield.

Key words: Wheat, Plant height, Dwarfing gene, Agronomic traits

表1

用于检测RhtB1-b、RhtD1-b及Rht8的分子标记"

检测基因名称
ID of detection gene		 正向引物序列(5′-3′)
Sequence of forward primer (5′-3′)		 反向引物序列(5′-3′)
Sequence of reverse primer (5′-3′)		 退火温度(℃)
Annealing temperature
RhtB1-b TCTCCTCCCTCCCCACCCAAC CATCCCCATGGCCATCTCGAGCTA 63
RhtD1-b CGCGCAATTATTGGCCAGAGATAG CCCCATGGCCATCTCGAGCTGCTA 63
Rht8 CTCCCTGTACGCCTAAGGC CTCGCGCTACTAGCCATTG 55

图1

RhtB1-b (a)和RhtD1-b (b)引物在部分参试品种（系）中的扩增结果 M．Marker;1．农林10号;2．郑麦583;3．圣麦197;4．新良3号;5．光泰68;6．徐麦0054;7．泉麦890;8．西农501。下同"

图2

Rht8 4种变异在测试品种（系）中的PCR扩增结果 9．丰德存麦12号;10．龙科1221。a．192bp;b．202bp;c．165bp;d．174bp"

图3

631份小麦品种（系）中RhtB1-b、RhtD1-b、Rht8单倍型的分布频率"

表2

631份小麦品种（系）材料农艺性状的描述性统计"

性状Trait 最小值
Minimum		 最大值
Maximum		 平均值
Mean		 标准差
Standard deviation		 变异系数(%)
Coefficient of variation
株高Plant height (cm) 59.30 91.00 76.99 4.51 0.06
每公顷穗数Spike number per hectare (×106) 3.80 6.63 5.57 0.45 0.08
千粒重1000-kernel weight(g) 37.00 53.40 45.00 2.71 0.06
穗粒数Kernel number per spike 29.10 43.80 34.80 2.19 0.06

图4

4个农艺性状的散点矩阵图 柱状图表示4个性状的频率分布;左下角元件显示两两性状间的二维散点图（直线拟合线）,右上角表示两两性状间的相关系数和显著性（“*” P<0.05）"

表3

Rht8对4个农艺性状的影响"

性状
Trait		 位点
Locus		 样本容量
Sample size		 平均数
Mean		 标准差
Standard error		 最小值
Minimum		 最大值
Maximum		 F 显著性
Significance
株高Plant height (cm) Rht8(+) 513 76.70 4.44 59.30 89.00 11.53 0.001**
Rht8(-) 117 78.26 4.61 63.90 91.00
每公顷穗数Spike number per hectare (×106) Rht8(+) 513 5.54 0.36 3.80 6.63 10.12 0.002**
Rht8(-) 117 5.66 0.48 4.40 6.63
千粒重1000-kernel weight (g) Rht8(+) 513 45.10 2.66 37.80 53.40 4.08 0.044*
Rht8(-) 117 44.54 2.90 37.00 50.90
穗粒数Kernel number per spike Rht8(+) 513 34.93 2.58 29.10 43.80 15.19 0.000**
Rht8(-) 117 34.06 2.41 29.40 40.61
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