作物杂志,2020, 第3期: 197–200 doi: 10.16035/j.issn.1001-7283.2020.03.030

• 植物保护 • 上一篇    

黑龙江省稻瘟病菌无毒基因的检测

孟峰, 张亚玲, 靳学慧()   

  1. 黑龙江八一农垦大学/黑龙江省作物-有害生物互作生物学及生态防控重点实验室/黑龙江省植物抗性研究中心,163319,黑龙江大庆
  • 收稿日期:2019-10-12 修回日期:2020-01-21 出版日期:2020-06-15 发布日期:2020-06-10
  • 通讯作者: 靳学慧 E-mail:Jxh2686@163.com
  • 作者简介:孟峰,主要从事植物病理学研究,E-mail: xmfengf@126.com
  • 基金资助:
    黑龙江八一农垦大学学成、引进人才科研启动计划(XDB-2016-05);黑龙江省自然科学基金(QC2011C046);黑龙江省农垦总局科技攻关项目(HNK125A-08-06);黑龙江省农垦总局科技攻关项目(HNK135-02-02);黑龙江省教育厅项目(12521376)

Detection of Avirulence Genes of Magnaporthe oryzea in Heilongjiang Province

Meng Feng, Zhang Yaling, Jin Xuehui()   

  1. Heilongjiang Bayi Agricultural University/Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control/Heilongjiang Plant Resistance Research Center, Daqing 163319, Heilongjiang, China
  • Received:2019-10-12 Revised:2020-01-21 Online:2020-06-15 Published:2020-06-10
  • Contact: Xuehui Jin E-mail:Jxh2686@163.com

摘要:

为了检测黑龙江省稻瘟病菌无毒基因ACE1、AVR-Pi9、AVR-PiaAVR-PiiAVR1-CO39的存在情况,采用5对无毒基因引物,对2017年黑龙江省208个稻瘟病菌单孢菌株DNA进行PCR扩增检测。结果表明,ACE1出现频率最高(87.98%);AVR1-CO39出现频率最低(0);无毒基因AVR-Pi9的出现频率为79.81%;AVR-Pia的出现频率为16.35%;AVR-Pii的出现频率为0.48%。黑龙江省稻瘟病菌无毒基因群体组成结构错综复杂,且具有明显的地域特点。

关键词: 黑龙江省, 稻瘟病菌, 无毒基因, 检测

Abstract:

In order to detect the presence of the avirulence genes ACE1, AVR-Pi9, AVR-Pia, AVR-Pii and AVR1-CO39 of Magnaporthe oryzae in Heilongjiang Province, the DNA of 208 strains of Magnaporthe oryzae in Heilongjiang Province was detected by PCR using 5 pairs avirulence gene primers. The results showed that the highest frequency of ACE1 was 87.98%; the frequency of AVR1-CO39 was 0; the frequency of avirulence gene AVR-Pi9 was 79.81%; the frequency of amplification of AVR-Pia was 16.35% and the amplification frequency of AVR-Pii was 0.48%. The composition of the avirulence gene population of Magnaporthe oryzae in Heilongjiang Province is complex and has obvious regional characteristics.

Key words: Heilongjiang Province, Magnaporthe oryzae, Avirulence gene, Detection

表1

用于扩增稻瘟病菌无毒基因的引物"

无毒基因
Avirulence
gene
引物序列 (5′-3′)
Primer sequence (5′-3′)
目的片段长度
Length of targeted fragment (bp)
ACE1 F: GTGGAATCTTGTGGTGCTACGG
R: ACGGGAACATCGACGGAAAG
1 057
AVR-Pi9 F: CGGCAGGTGGGTTTATCATC
R: CCAAAGCAGACAGCGACTCTA
1 070
AVR-Pia F: TAGCCATTTCGGTCTCAT
R: CGGGTAAACCCTGTCATA
1 597
AVR-Pii F: TATGCAGGCCCAAATCCGTAG
R: CCCGCAATAGTCCATAACTGTCC
526
AVR1-CO39 F: CCTTGCCGCTACTGAAAC
R: CCCTATCGCAGAGGAACA
419

表2

2017年黑龙江省稻瘟病菌无毒基因的扩增频率"

无毒基因
Avirulent gene
出现个数
Number of occurrences
出现频率
Occurrence frequency (%)
ACE1 183 87.98
AVR-Pi9 166 79.81
AVR-Pia 34 16.35
AVR-Pii 1 0.48
AVR1-CO39 0 0.00

表3

黑龙江省不同地区无毒基因分布情况"

序号
No.
地区Area 总数
Total
ACE1 AVR-Pi9 AVR-Pia AVR-Pii AVR1-CO39
数目
Number
频率(%)
Frequency
数目
Number
频率(%)
Frequency
数目
Number
频率(%)
Frequency
数目
Number
频率(%)
Frequency
数目
Number
频率(%)
Frequency
S1 齐齐哈尔甘南 16 15 93.75 14 87.50 0 0.00 0 0.00 0 0
S2 齐齐哈尔哈拉海农场 6 5 83.33 5 83.33 4 66.67 0 0.00 0 0
S3 大庆杜蒙 19 18 94.74 13 68.42 0 0.00 0 0.00 0 0
S4 大庆肇源 11 11 100.00 11 100.00 0 0.00 0 0.00 0 0
S5 绥化望奎 7 7 100.00 7 100.00 4 57.14 0 0.00 0 0
S6 绥化庆安 14 13 92.86 13 92.86 8 57.14 0 0.00 0 0
S7 哈尔滨通河 16 12 75.00 11 68.75 3 18.75 0 0.00 0 0
S8 哈尔滨依兰 23 21 91.30 21 91.30 0 0.00 0 0.00 0 0
S9 哈尔滨方正 4 4 100.00 4 100.00 0 0.00 0 0.00 0 0
S10 哈尔滨尚志 10 10 100.00 10 100.00 1 10.00 0 0.00 0 0
S11 佳木斯桦川 10 9 90.00 5 50.00 0 0.00 0 0.00 0 0
S12 佳木斯汤原 22 15 68.18 16 72.73 8 36.36 0 0.00 0 0
S13 佳木斯桦南 15 13 86.67 13 86.67 0 0.00 0 0.00 0 0
S14 鹤岗绥滨 16 16 100.00 13 81.25 3 18.75 0 0.00 0 0
S15 鸡西虎林 9 5 55.56 5 55.56 3 33.33 0 0.00 0 0
S16 鸡西密山 10 9 90.00 5 50.00 0 0.00 1 10.00 0 0

图1

各无毒基因在黑龙江省不同地区出现频率比较"

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