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作物杂志,2021, 第3期: 51–56 doi: 10.16035/j.issn.1001-7283.2021.03.008

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

四川豌豆种质资源白粉病抗性及分子鉴定

项超1(), 孙素丽2, 朱振东2, 宗绪晓2(), 杨涛2, 刘荣2, 杨梅1, 鲜东锋1, 杨秀燕1   

  1. 1四川省农业科学院作物研究所,610066,四川成都
    2中国农业科学院作物科学研究所,100081,北京
  • 收稿日期:2020-12-29 修回日期:2021-01-29 出版日期:2021-06-15 发布日期:2021-06-22
  • 通讯作者: 宗绪晓
  • 作者简介:项超,主要从事豆类种质资源与遗传育种,E-mail： xc2011cib@163.com
  • 基金资助:
    国家食用豆产业技术体系(CARS-08);四川省科技计划项目(2018JY0152);四川省财政创新能力提升工程项目(2016ZYPZ-017);四川省财政创新能力提升工程项目(2018QNJJ-029);第十六批“西部之光”访问学者人才计划项目

Resistance and Molecular Identification to Powdery Mildew of Pea Germplasms in Sichuan

Xiang Chao1(), Sun Suli2, Zhu Zhendong2, Zong Xuxiao2(), Yang Tao2, Liu Rong2, Yang Mei1, Xian Dongfeng1, Yang Xiuyan1   

  1. 1Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, Sichuan, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2020-12-29 Revised:2021-01-29 Online:2021-06-15 Published:2021-06-22
  • Contact: Zong Xuxiao

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摘要：

豌豆白粉病是由白粉菌（Erysiphe pisi D. C.）引起的豌豆最重要的病害之一,控制豌豆白粉病最经济有效且环保的方法是种植抗病品种。在温室条件下对400份豌豆种质资源进行白粉病抗性鉴定,同时利用7个与已知豌豆抗白粉病基因连锁的分子标记进行基因型鉴定。结果表明,在400份资源中,有8份表现免疫,3份表现抗病,5份表现抗感分离,其余384份均为感病;16份抗性资源中有10份来自于四川省中部不同纬度地区,其余6份为国外引进资源;7个分子标记将400份种质资源分为39个标记基因型,其中16份抗性资源分为7个标记基因型。上述抗性资源及其标记基因型可有效应用于豌豆白粉病抗性育种的研究中。

关键词: 豌豆, 白粉病, 种质资源, 抗性鉴定

Abstract:

Powdery mildew caused by Erysiphe pisi D. C. is one of the most important diseases of pea. The most economical, effective and environmentally friendly way to control powdery mildew of pea is to plant resistant varieties. A total of 400 pea germplasm resources were identified for the resistance to powdery mildew under greenhouse conditions. At the same time, seven molecular markers linked to known powdery mildew resistance genes of pea were used for genotype identification. The results showed that among the 400 identified germplasms, eight accessions showed immunity, three accessions showed resistance, five accessions showed resistance-susceptibility separation, and the remaining 384 accessions were susceptible. Among the 16 resistant accessions, ten accessions were from different latitudes in central of Sichuan and the remaining six accessions were imported from abroad. Seven molecular markers divided the 400 germplasms into 39 marker genotypes and 16 resistant germplasms were divided into seven marker genotypes. The resistant resources and their marker genotypes described above could be effectively used for the breeding of pea resistant to powdery mildew.

Key words: Pea, Powdery mildew, Germplasm resources, Resistance identification

表1

用于豌豆资源标记基因型鉴定的分子标记

分子标记
Molecular
marker		 引物序列
Primer sequence		 退火温度
Annealing temperature (°C)		 遗传距离
Genetic
distance (cM)		 条带大小
Band
size (bp)		 基因
Gene		 来源文献
Reference
ScOPD-10650
 F: GGTCTACACCTAAACAGTGTCCGT
R: GGTCTACACCTCATATCTTGATGA		 58.0 2.1 650 er1 Timmerman等[17],Tiwari等[18]
ScOPE-161600
 F: GGTGACTGTGGAATGACAAA
R: GGTGACTGTGACAATTCCAG		 55.0 4.0±2.0 1600 er1 Tiwari等[18]
ScOPO-181200
 F: CCCTCTCGCTATCCAATCC
R: CCTCTCGCTATCCGGTGTG		 60.0 0.0 1200 Er1 Tiwari等[18]
ScOPX-04880
 F: CCGCTACCGATGTTATGTTTG
R: CCGCTACCGAACTGGTTGGA		 58.0 0.6 880 Er1 Srivastava等[19]
ScX17_1400
 F: GGACCAAGCTCGGATCTTTC
R: GACACGGACCCAATGACATC		 65.0 2.6 1400 er2 Katoch等[20]
SCW4637
 F: CAGAAGCGGATGAGGCGGA
R: CAGAAGCGGATACAGTACTAAC		 55.0 0.0 637 Er3 Fondevilla等[21]
SCAB1874
 F: CCGTCGGTAGTAAACTA
R: CCGTCGGTAGCCACACCA		 59.5 2.8 874 Er3 Fondevilla等[21]

图1

抗病、免疫和感病植株对比

表2

四川抗性资源分布

名称
Name		 编号
No.		 抗性
Resistance		 地点
Location
昭化麻-1 Zhaohuama-1 179 R/S 昭化
绵阳花Mianyanghua 209 R/S 绵阳
金堂黄麻-2 Jintanghuangma-2 329 I 金堂
大邑白豌豆Dayibaiwandou 382 I 大邑
眉山菜Meishancai 248 R/S 眉山
犍为大绿Qianweidalü 256 I 犍为
高县大麻-2 Gaoxiandama-2 168 R 高县
会理大菜豌Huilidacaiwan 100 R 会理
会理大白Huilidabai 98 R/S 会理
会理大白壳Huilidabaike 99 R/S 会理

图2

7个与白粉病抗性基因连锁的SCAR标记扩增条带 369：食荚小菜豌3号,370：食荚甜脆豌1号-1,371：成豌7号,372：食荚大菜豌1号-2,373：食荚甜脆豌1号-2,374：成豌8号（对照）,375：成豌9号,376：青豌豆,M：DL 2000 Marker

表3

7个分子标记扩增条带资源份数

标记
Marker		 扩增出条带数
Number of accessions
with amplified fragment		 占比
Proportion
(%)		 基因
Gene
ScOPD-10650 365 91.25 er1
ScOPE-161600 334 83.50 er1
ScOPO-181200 350 87.50 Er1
ScOPX-04880 367 91.75 Er1
ScX17_1400 359 89.75 er2
SCW4637 88 22.00 Er3
SCAB1874 394 98.50 Er3

图3

400份豌豆种质资源抗白粉病标记基因型鉴定聚类分析 G1~G39表示39个标记基因型,括号内第一个数字表示资源份数,第二个数字表示抗性资源份数,红线表示存在抗性资源

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