外源黄体酮影响小麦根系伸长生长的生理调控途径

doi:10.16035/j.issn.1001-7283.2026.02.019

摘要/Abstract

摘要：

黄体酮是一种存在于植物体中的类固醇激素，与植物的生长发育紧密相关，对植物抵抗逆境胁迫也有积极作用。小麦根系生长状况直接影响产量与抗逆能力，本试验研究了外源黄体酮对小麦根系伸长生长的影响，并初步分析其调控途径。结果表明，不同浓度外源黄体酮对小麦根系生长的调控效应存在差异，低浓度（0.001和0.01 μmol/L）促进根系伸长生长，高浓度（0.1和1.0 μmol/L）抑制其生长。外源黄体酮处理显著影响小麦幼苗根系中的葡萄糖含量及磷酸果糖激酶（PFK）活性，随着黄体酮处理浓度升高，根系葡萄糖含量先降后升，而PFK活性变化趋势与之相反。进一步研究发现，10 μmol/L葡萄糖处理下幼苗根系葡萄糖含量降低，PFK活性升高；10 000 μmol/L葡萄糖处理下幼苗根系葡萄糖含量升高，PFK活性受到抑制。此外，添加0.1 μmol/L黄体酮可显著促进低浓度葡萄糖处理下根系葡萄糖的积累，并抑制葡萄糖对PFK活性的诱导作用；而添加0.001 μmol/L黄体酮能显著抑制高浓度葡萄糖处理下根系葡萄糖的累积，缓解葡萄糖对PFK活性的抑制作用。外源黄体酮可能通过作用于糖酵解途径的关键限速酶PFK影响糖酵解过程，进而调控根系中葡萄糖含量，实现对小麦根系伸长生长的调控。

关键词: 小麦, 根, 黄体酮, 葡萄糖, 磷酸果糖激酶

Abstract:

Progesterone is a steroid hormone found in plants, which is closely related to plant growth and development and plays a positive role in stress resistance. The growth status of wheat roots directly affects yield and stress resistance. This study investigated the effects of exogenous progesterone on the elongation growth of wheat roots and preliminarily analyzed their regulatory pathways. The results showed that different concentrations of exogenous progesterone exerted varying regulatory effects on wheat root growth: low concentrations (0.001 and 0.01 μmol/L) promoted root elongation, whereas high concentrations (0.1 and 1.0 μmol/L) inhibited growth. Exogenous progesterone treatment significantly affected the glucose content and phosphofructokinase (PFK) activity in the roots of wheat seedlings. With increasing progesterone concentration, the root glucose content first decreased and then increased, while the trend of PFK activity was opposite. Further research found that under 10 μmol/L glucose treatment, the root glucose content decreased and PFK activity increased; under 10 000 μmol/L glucose treatment, the root glucose content increased and PFK activity was inhibited. In addition, the application of 0.1 μmol/L progesterone significantly promoted root glucose accumulation under low-concentration glucose treatment and inhibited the induction effect of glucose on PFK activity. Conversely, the application of 0.001 μmol/L progesterone significantly inhibited root glucose accumulation under high-concentration glucose treatment and alleviated the inhibitory effect of glucose on PFK activity. These findings suggest that exogenous progesterone may affect the glycolysis process by acting on PFK, a key rate-limiting enzyme in the glycolytic pathway, thereby regulating the glucose content in roots and regulating wheat root elongation growth.

Key words: Wheat, Root, Progesterone, Glucose, Phosphofructokinase

王丹, 王润, 胡孝庆, 于会勇, 李江涛, 程星, 郭海悦, 刘婷, 胡珍珍, 李华. 外源黄体酮影响小麦根系伸长生长的生理调控途径[J]. 作物杂志, 2026 (2): 154–159

Wang Dan, Wang Run, Hu Xiaoqing, Yu Huiyong, Li Jiangtao, Cheng Xing, Guo Haiyue, Liu Ting, Hu Zhenzhen, Li Hua. Physiological Regulation Pathways of Exogenous Progesterone Affecting Wheat Root Elongation and Growth[J]. Crops, 2026(2): 154–159

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