镉胁迫对烤烟农艺性状及生理生化指标的影响

doi:10.16035/j.issn.1001-7283.2017.05.026

摘要/Abstract

摘要：

为明确重金属镉（Cd）胁迫对烤烟农艺性状和生理生化指标的影响,以吉烟9号为供试品种,采用盆栽方法,模拟土壤不同浓度Cd胁迫对烤烟农艺性状及生理生化指标的影响。试验结果表明,Cd胁迫下,烤烟叶长、叶宽、株高、茎围在Cd>3mg/kg时随Cd浓度增加而下降（Cd20处理叶宽除外）。CAT、SOD活性随Cd浓度增加先升后降;Pro含量变化趋势则相反;POD活性和MDA含量持续升高,而Chl含量则持续下降。总氮、蛋白质、总糖、还原糖含量均随Cd浓度的升高呈先升后降再升的趋势;烟碱含量随Cd浓度升高逐渐降低。

关键词: 烤烟, Cd胁迫, 农艺性状, 生理生化指标

Abstract:

In order to clarify the effects of heavy metal (Cd) stress on agronomic traits and physiological and biochemical indexes of flue-cured tobacco, the agronomic traits and physiological and biochemical indexes were determined under different concentration Cd stress for cultivated species Jiyan 9 of flue-cured tobacco using potting method in the experiment. The results showed that, leaf length, leaf width, plant height and stem circumference decreased when Cd>3mg/kg (except the leaf width of Cd20 treatment). The activity of catalase (CAT) and superoxide dismutase (SOD) increased first and then decreased with the increase of Cd concentration, which was opposite to the content of proline (Pro). The activity of peroxidase (POD) and content of Malondialdehyde (MDA) increased while the content of chlorophyll (Chl) decreased continuously, the content of total nitrogen, protein, total sugar and reducing sugar increased first and then decreased but then increased again with the increase of Cd concentration. The content of nicotine decreased with the increase of Cd concentration.

Key words: Flue-cured tobacco, Cd stress, Agronomic traits, Physiological and biochemical indexes

闫寒,宋杭霖,张丽,闫晶,石贤吉,朱诗苗,刘璐,李虎林. 镉胁迫对烤烟农艺性状及生理生化指标的影响[J]. 作物杂志, 2017 (5): 156–161

Han Yan,Hanglin Song,Li Zhang,Jing Yan,Xianji Shi,Shimiao Zhu,Lu Liu,Hulin Li. Effects of Cadmium Stress on Agronomic Traits and Physiological and Biochemical Indexes of Flue-Cured Tobacco[J]. Crops, 2017(5): 156–161

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参考文献 17

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处理Treatment	叶长Leaf length (cm)	叶宽Leaf width (cm)	株高Plant height (cm)	茎围Stem girth (cm)	叶片数Leaf No. (个)
CK	59.96±1.73bABC	25.44±0.00abA	110.00±0.75bBC	7.80±0.06abA	19.78±1.03abA
Cd1	62.00±1.15abAB	26.60±0.35aA	114.47±0.73aAB	7.93±0.04abA	18.67±0.73bA
Cd3	65.67±0.19aA	29.73±1.34aA	116.33±1.73aA	8.13±0.08aA	19.67±0.19abA
Cd6	60.13±0.08bABC	26.60±1.33aA	110.33±0.81bBC	7.80±0.46abA	20.00±0.00abA
Cd10	59.53±3.00bABC	24.67±0.79abA	110.00±0.58bBC	7.67±0.68abA	19.33±0.19abA
Cd20	58.00±0.58bcBC	24.73±3.13bA	108.67±1.39bC	7.33±0.19abA	20.67±0.06aA
Cd40	54.80±0.35cC	24.20±1.21abA	94.67±0.39cD	6.87±0.06bA	19.00±0.00bA

浓度Concentration	Chl含量 Chl content (μg/gFW)	CAT活性 CAT activity [U/(min·gFW)]	POD活性 POD activity [U/(min·gFW)]	SOD活性 SOD activity [U/(min·gFW)]	MDA含量 MDA content (μmol/gFW)	可溶性糖含量 Soluble sugar content (μmol/gFW)	Pro含量 Pro content (μg/gFW)
CK	41.02±0.07aA	41.24±0.13cC	307.93±0.17gG	135.84±0.06gG	9.10±0.71eE	11.97±0.16dC	310.72±0.09aA
Cd1	38.01±1.44bB	54.50±0.00bB	314.94±0.23fF	160.67±0.12bfF	10.03±0.15dDE	12.91±0.01cBC	288.47±0.04cC
Cd3	34.26±0.26cC	56.46±0.03aA	327.86±0.36eE	238.51±0.09bB	11.14±0.20cCD	14.00±0.58bB	275.64±0.19fF
Cd6	32.46±0.14cCD	24.26±0.03dD	341.84±0.15dD	277.29±0.10aA	11.52±0.17cC	17.85±0.29aA	251.49±0.01gG
Cd10	30.31±0.03dDE	16.13±0.01eE	390.13±0.08cC	205.83±0.03cC	13.00±0.06bB	16.99±0.10aA	276.58±0.12eE
Cd20	28.31±0.18eEF	12.69±0.10fF	402.16±0.29bB	202.45±0.17dD	13.61±0.01bB	17.00±0.13aA	286.58±0.05dD
Cd40	27.34±0.52eF	9.94±0.31gG	458.54±0.19aA	188.27±0.05eE	16.57±0.13aA	17.25±0.42aA	295.09±0.28bB

部位Position	处理Treatment	总氮Total nitrogen	蛋白质Protein	烟碱Nicotine	还原糖Reducing sugar	总糖Total sugar
上部叶Upper leaf	CK	2.08aA	9.05bB	2.59aA	17.36fD	20.34dD
	Cd1	2.14aA	9.48bB	2.31bB	20.36bB	20.50cdCD
	Cd3	2.26aA	10.26aA	2.20bcBC	25.21aA	27.05aA
	Cd6	2.27aA	10.33aA	1.99cdC	19.98cB	22.17bB
	Cd10	2.11aA	9.25bB	1.97dC	19.94cB	22.09bB
	Cd20	2.11aA	9.24bB	1.91dC	17.94eC	20.62cCD
	Cd40	2.11aA	9.25bB	1.89dC	18.34dC	20.74cC
中部叶Middle leaf	CK	2.13aA	9.26cB	2.50aA	18.66cC	20.99bB
	Cd1	2.23aA	9.84bcAB	2.33aA	18.73cC	21.00bB
	Cd3	2.34aA	10.51abA	2.10aA	26.48aA	28.14aA
	Cd6	2.36aA	10.79aA	1.88aA	20.84bB	21.86bB
	Cd10	2.23aA	9.98abcAB	1.85aA	20.81bB	21.81bB
	Cd20	2.23aA	9.90bcAB	1.80aA	18.81cC	21.04bB
	Cd40	2.30aA	10.24abAB	1.80aA	19.31cC	21.55bB
下部叶Lower leaf	CK	2.02aA	9.00bC	1.72aA	16.78eE	21.07dD
	Cd1	2.19aA	10.09abAB	1.53abAB	21.31bB	27.07aA
	Cd3	2.28aA	10.66aA	1.34bcBC	24.52aA	27.43aA
	Cd6	2.29aA	10.71aA	1.12cCD	19.90cC	24.94bB
	Cd10	2.12aA	9.61abBC	1.09cD	19.88cC	24.24bB
	Cd20	2.12aA	9.62abBC	1.04cD	17.82dD	21.81cdD
	Cd40	2.21aA	10.23abAB	1.03cD	18.35dD	22.83cC