不同氮效率水稻品种叶片光合作用及氮利用特征的差异分析

doi:10.16035/j.issn.1001-7283.2019.01.015

摘要/Abstract

摘要：

以扬稻6（氮高效品种）、武玉粳3（氮低效品种）为材料,在含有2.86mmol/L NH₄ ⁺溶液培养条件下,分别在开花期和灌浆期分析了中位叶、旗叶的净光合速率、氮同化酶的活性、叶片氮含量与转移率以及对子粒的贡献。结果表明：（1）旗叶的净光合速率显著高于中位叶;扬稻6旗叶的净光合速率显著高于武玉粳3,而中位叶净光合速率在品种间差异不显著。（2）硝酸还原酶活性（NRA）、谷氨酰胺合成酶活性（GSA）是中位叶高于旗叶、灌浆期高于开花期、扬稻6高于武玉粳3。从开花到灌浆期,旗叶可溶性糖含量呈增加的趋势,而中位叶可溶性糖含量呈减少的趋势。旗叶可溶性糖含量高于中位叶、扬稻6高于武玉粳3。可溶性蛋白含量呈下降的趋势,扬稻6可溶性蛋白含量高于武玉粳3。扬稻6、武玉粳3中位叶可溶性蛋白的转出率分别是50.3%、37.6%;旗叶可溶性蛋白的转出率分别是69.5%、44.4%。（3）中位叶总氮积累量显著高于旗叶、开花期显著高于灌浆期。扬稻6中位叶、旗叶氮转移量分别为35.6%、34.7%;武玉粳3中位叶、旗叶氮转移量分别为26.9%、35.3%。（4）去除中位叶,子粒氮分别比对照减少了6.9%（扬稻6）、4.6%（武玉粳3）;子粒产量分别比对照减少了7.3%（扬稻6）、4.9%（武玉粳3）。去除旗叶,子粒氮分别比对照减少了10.5%（扬稻6）和9.2%（武玉粳3）;子粒产量分别比对照减少了13.8%（扬稻6）、11.1%（武玉粳3）,表明旗叶对子粒氮和产量的贡献更大。总之,氮高效品种旗叶光合作用和氮同化能力显著高于氮低效品种,另一方面,其中位叶储存的氮也能及时转移到子粒中,提高了氮效率,这反映了不同氮效率品种氮素利用差异的机制。

关键词: 水稻品种, 叶片, 光合作用, 氮素利用效率

Abstract:

Two rice cultivars,Yangdao 6 (a cultivar with high N use efficiency) and Wuyujing 3 (a cultivar with low N use efficiency) were hydroponically grown in nutrient solutions containing 2.86mmol/L NH₄ ⁺. Several physiological indexes in mid-position and flag leaves, such as net photosynthetic rate, the activities of glutamine synthetase (GS) and nitrate reductase (NR), content of soluble sugar and protein, content and transition of N and its contribution to grain N accumulation and yields were conducted. The results were as follows: (1) Net photosynthetic rate of flag leaves were always higher than that of mid-position leaves at the stage of anthesis or grain filling. As for cultivars, net photosynthetic rate of flag leaves in Yangdao 6 was significantly higher than that in Wuyujing 3 (P<0.05), while it showed no significant differences between the mid-position leaves at the stage of anthesis. Furthermore, it was higher in flag leaves than that in mid-position leaves and higher in Yangdao 6 than that in Wuyujing 3. (2) NRA and GSA were higher in mid-position leaves than that in flag leaves and were higher at grain filling stage than at anthesis stage. Also, they were significantly higher in Yangdao 6 than that in Wuyujing 3. From flowering to grain filling stage, content of soluble sugar exhibited increase in flag leaves while decrease in mid-position leaves, respectively. Content of soluble protein decreased, and it was higher in Yangdao 6 than that in Wuyujing 3. The transported soluble protein in mid-position and flag leaves in Yangdao 6, Wuyujing 3 were 50.3%, 37.6% and 69.5%, 44.4%, respectively. (3) Total N accumulation in mid-position leaves was higher than that in flag leaves and was higher at anthesis stage than at grain filling stage. As for cultivars, the amount of total N accumulation and transition in mid-position and flag leaves in Yangdao 6, Wuyujing 3 were 35.6% and 34.7%, 26.9% and 35.3%, respectively. (4) Grain N content and grain yields were significantly reduced under treatments of cutting leaves. Grain N content was decreased by 6.9% in Yangdao 6 and 4.6% in Wuyujing 3 when removed mid-position leaves and decreased by 10.5% in Yangdao 6 and 9.2% in Wuyujing 3 when removed flag leaves. Grain yields were decreased by 7.3% in Yangdao 6 and 4.9% in Wuyujing 3 when removed mid-position leaves and decreased by 13.8% in Yangdao 6 and 11.1% in Wuyujing 3 when removed flag leaves. This suggested that flag leaves contributed largely to the accumulation of grain N and the production of yields. In general, net photosynthesis rate and N assimilation were significantly higher in cultivars with high N use efficiency than those in cultivars with low N use efficiency. On the other hand, N stored in mid-position leaves in cultivars with high N use efficiency can be transferred to the kernel to reutilize, thereby, N use efficiency was improved. This indicated there were different mechanisms in the N use efficiency between various rice cultivars.

Key words: Rice cultivars, Leaves, Photosynthesis, Nitrogen use efficiency

尹晓明,李辰. 不同氮效率水稻品种叶片光合作用及氮利用特征的差异分析[J]. 作物杂志, 2019 (1): 90–96

Xiaoming Yin,Chen Li. Differences in Leaf Photosynthesis and Assimilation of Nitrogen Between Two Rice Cultivars Differing in Nitrogen Use Efficiency[J]. Crops, 2019(1): 90–96

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参考文献 25

[1]	陈颖, 周振翔, 周天阳 . 水稻氮高效吸收利用机制及栽培调控措施. 作物杂志, 2017(6):26-32. doi: 10.16035/j.issn.1001-7283.2016.06.005
[2]	Xue Y G, Duan H, Liu L J ,et al. An improved crop management increases grain yield and nitrogen and water use efficiency in rice. Crop Science, 2013,53(1):271-284. doi: 10.2135/cropsci2012.06.0360
[3]	Zhang W F, Dan Z X, He P ,et al. New technologies reduce greenhouse gas emissions from nitrogenous fertilizer in China. Proceedings of the National Academy of Sciences of the United States of America, 2013,110(211):8375-8380. doi: 10.1073/pnas.1210447110
[4]	江立庚, 戴廷波, 韦善清 , 等. 南方水稻氮素吸收与利用效率的基因型差异及评价. 植物生态学报, 2003,27(4):466-471. doi: 10.17521/cjpe.2003.0067
[5]	张荣萍, 陶诗顺 . 不同基因型水稻产量和氮效率对减量施肥反应的差异性研究. 湖南师范大学自然科学学报, 2016,39(5):27-37. doi: 10.7612/j.issn.1000-2537.2016.05.005
[6]	Tabuchi M, Abiko T, Yamaya T . Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.). Journal of Experimental Botany, 2007,58(9):2319-2327. doi: 10.1093/jxb/erm016 pmid: 17350935
[7]	Singh U, Ladha J K, Castillo E G , et al. Genotypic variation in nitrogen use efficiency in medium- and long-duration rice. Field Crops Research, 1998,58:35-53. doi: 10.1016/S0378-4290(98)00084-7
[8]	Mae T, Ohira K . The remobilization of nitrogen related to leaf growth and senescence in rice plants (Oryza sativa L.). Plant Cell Physiology, 1981,22:1067-1074. doi: 10.1016/0303-7207(81)90019-8
[9]	魏爱丽, 王志敏 . 小麦不同光合器官对穗粒重的作用及基因型差异研究. 麦类作物学报, 2001,21(2):57-61. doi: 10.7606/j.issn.1009-1041.2001.02.043
[10]	陈冬梅, 马永安, 苏玉环 , 等. 不同落黄型小麦品种光合器官衰老及产量对花后高温的响应. 麦类作物学报, 2017,37(12):1596-1603. doi: 10.7606/j.issn.1009-1041.2017.12.10
[11]	孙书娈, 陈秀敏, 乔文臣 , 等. 矮秆、早熟、大穗大粒冬小麦品种衡观35籽粒干物质积累初步研究. 华北农学报, 2010,25(S):157-161. doi: 10.7668/hbnxb.2010.S1.036
[12]	董明辉, 赵步洪, 陈培峰 . 机插方式对杂交粳稻碳氮调节与颖花形成及产量的影响. 农业工程学报, 2017,33(13):65-73. doi: 10.11975/j.issn.1002-6819.2017.13.009
[13]	Li Y L, Fan X R, Shen Q R . The relationship between rhizosphere nitrification and nitrogen-use efficiency in rice plants. Plant Cell and Environment, 2008,31:73-85. doi: 10.1111/j.1365-3040.2007.01737.x pmid: 17944815
[14]	李合生 . 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 125-127.
[15]	郭增江, 于振文, 石玉 , 等. 不同土层测墒补灌对小麦旗叶光合特性和干物质积累与分配的影响. 作物学报, 2014,40(4):731-738. doi: 10.3724/SP.J.1006.2014.00731
[16]	周苏玫, 张珂珂, 张嫚 , 等. 减氮适墒提高冬小麦旗叶光合潜力和籽粒产量. 作物学报, 2016,42(11):1677-1688. doi: 10.3724/SP.J.1006.2016.01677
[17]	王碧茜, 范晓荣, 徐国华 , 等. 不同氮效率水稻品种旗叶的衰老特征. 南京农业大学学报, 2010,33(2):8-12. doi: 10.7685/j.issn.1000-2030.2010.02.002
[18]	Zhang Y L, Lü H J, Wang D S , et al. Partial nitrate nutrition amends photosynthetic characteristics in rice (Oryza sativa L. var. japonica) differing in nitrogen-use efficiency. Plant Growth Regulation, 2011,63:235-242. doi: 10.1007/s10725-010-9520-7
[19]	江文文, 尹燕枰, 卢昆丽 , 等. 花后高温胁迫下氮肥追施后移对小麦产量及旗叶生理特性的影响. 作物学报, 2014,40(5):942-949. doi: 10.3724/SP.J.1006.2014.00942
[20]	宁书菊, 陈晓飞, 张国英 , 等. 水稻生育后期剑叶氮代谢相关酶活性及动力学变化. 中国生态农业学报, 2012,20(12):1606-1613. doi: 10.3724/SP.J.1011.2012.01606
[21]	路文静, 张树华, 郭程瑾 , 等. 不同氮素利用效率小麦品种的氮效率相关生理参数研究. 植物营养与肥料学报, 2017(5):985-991.
[22]	Fan X R, Shen Q R, Ma Z Q , et al. A comparison of nitrate transport in four different rice (Oryza sativa L.) cultivars. Science in China Ser. C Life Sciences, 2005,48:897-911.
[23]	Duan Y H, Zhang Y L, Ye L T , et al. Responses of rice cultivars with different nitrogen use efficiency to partial nitrate nutrition. Annals of Botany, 2007,99(6):1153-1160. doi: 10.1093/aob/mcm051 pmid: 3244343
[24]	Fan X R, Shen Q R, Li Y L , et al. Comparing nitrate storage and remobilization in two rice cultivars that differ in their nitrogen use efficiency. Journal of Experimental Botany, 2007,58:1729-1740. doi: 10.1093/jxb/erm033 pmid: 17351248
[25]	王绍华, 吉志军, 刘胜环 , 等. 水稻氮素供需差与不同叶位叶片氮转运和衰老的关系. 中国农业科学, 2003,36(11):1261-1265. doi: 10.3321/j.issn:0578-1752.2003.11.005

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