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作物杂志,2019, 第3期: 55–65 doi: 10.16035/j.issn.1001-7283.2019.03.010

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

大豆PHD家族蛋白的全基因组鉴定及表达特征分析

杨珺凯,沈阳,才晓溪,邬升杨,李建伟,孙明哲,贾博为,孙晓丽   

  1. 黑龙江八一农垦大学农学院/作物逆境分子生物学实验室,163319,黑龙江大庆
  • 收稿日期:2018-10-30 修回日期:2019-02-27 出版日期:2019-06-15 发布日期:2019-06-12
  • 通讯作者: 孙晓丽
  • 作者简介:杨珺凯,硕士研究生,研究方向为作物遗传育种;
  • 基金资助:
    国家自然科学基金面上项目(31671596);中国博士后科学基金特别资助项目(2017T100253);黑龙江八一农垦大学研究生创新科研项目(YJSCX2017-Y08)

Genome-Wide Identification and Expression Patterns Analysis of the PHD Family Protein in Glycine max

Yang Junkai,Shen Yang,Cai Xiaoxi,Wu Shengyang,Li Jianwei,Sun Mingzhe,Jia Bowei,Sun Xiaoli   

  1. College of Agronomy, Heilongjiang Bayi Agricultural University/Crop Stress Molecular Biology Laboratory, Daqing 163319, Heilongjiang, China
  • Received:2018-10-30 Revised:2019-02-27 Online:2019-06-15 Published:2019-06-12
  • Contact: Xiaoli Sun

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摘要：

PHD（Plant Homeodomain Finger）基因家族编码一类锌指转录因子,广泛参与植物的生长发育和逆境应答过程。通过全基因组鉴定获得了95个大豆PHD家族蛋白。通过共线性分析、进化树构建、基因结构和功能结构域鉴定、GO注释分析、不同组织间和非生物胁迫下表达分析等,获得了大豆PHD家族基因复制、家族进化、保守结构域及基因表达等信息。结果表明,大豆PHD基因在家族进化、基因结构和保守结构域上存在较大变异,可能参与Zn 2+结合、DNA结合及表观遗传调控等分子过程,参与调控植物生长发育和逆境应答。这些结果为进一步研究大豆PHD家族在生长发育和逆境应答中的生物学功能提供重要线索。

关键词: 大豆, PHD转录因子, PHD家族分析, 表达特征, 逆境胁迫

Abstract:

PHD (Plant Homeodomain Finger) family genes encode a class of zinc finger transcription factor proteins, which are widely involved in regulating plant growth and stress responses. In this study, through genome-wide analysis, we identified a total of 95 PHD family proteins in soybean proteome. We further performed the synteny analysis, constructed the phylogenic tree, identified the gene structures and functional domains, analyzed the gene ontology (GO) annotation, and investigated the expression patterns in different tissues and under abiotic stresses. These data provided comprehensive information regarding the gene duplication, family evolution, conserved domains and gene expression of the soybean PHD family. Our results presented in this study suggest that soybean PHD family displays a great divergence in family evolution, gene structure and conserved domain organization; possibly participates in different molecular processes, including Zn 2+ binding, DNA binding and epigenetic regulation; and probably controls plant development and environmental stress responses. In summary, these findings will provide a crucial cue for further characterizing the biological function of soybean PHD family in plant growth and environmental stress responses.

Key words: Soybean, PHD transcription factor, Gene family analysis, Expression patterns, Environmental stress

图1

大豆PHD家族蛋白PHD结构域氨基酸序列比对"

图2

大豆PHD家族基因染色体定位和基因复制分析"

图3

大豆PHD家族基因进化关系分析"

图4

大豆PHD家族基因结构分析"

图5

大豆PHD家族蛋白保守结构域分析"

图6

大豆PHD家族基因本体映射"

图7

大豆PHD家族基因组织表达特性分析"

图8

大豆PHD家族基因在逆境胁迫下的表达模式分析"

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