检索
E-mail
RSS
高级检索 图表检索

当期目录 我要投稿 过刊浏览
高级检索 图表检索

作物杂志,2022, 第2期: 143–152 doi: 10.16035/j.issn.1001-7283.2022.02.020

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

叶面喷施钙素对光胁迫条件下烤烟光合生理特性的调控效应

云菲1(), 任天宝1,*(), 殷全玉1, 靳双珍2, 郑聪3, 金磊4, 李静静4, 刘国顺1, 杨喜田5,*()   

  1. 1河南农业大学烟草学院/河南省生物炭工程技术研究中心,450002,河南郑州
    2云南省烟草公司大理州公司,671000,云南大理
    3福建省烟草公司南平市公司,353100,福建南平
    4河南省烟草公司济源市公司,454650,河南济源
    5河南农业大学林学院,450002,河南郑州
  • 收稿日期:2021-08-25 修回日期:2021-10-09 出版日期:2022-04-15 发布日期:2022-04-24
  • 通讯作者: 任天宝,杨喜田
  • 作者简介:云菲,主要从事烟草栽培生理生态研究,E-mail： yunfeifei55@126.com
  • 基金资助:
    河南省自然科学基金(212300410160);河南省科技攻关计划（高新技术领域）(172102210306);福建省烟草公司南平市公司项目(南烟司叶[2017]21号);国家重点研发计划(2017YFD0200808)

Effects of Calcium by Foliage Spraying on Photosynthesis Physiology Characteristics of Flue-Cured Tobacco under Light Stress

Yun Fei1(), Ren Tianbao1,*(), Yin Quanyu1, Jin Shuangzhen2, Zheng Cong3, Jin Lei4, Li Jingjing4, Liu Guoshun1, Yang Xitian5,*()   

  1. 1College of Tobacco Science, Henan Agricultural University/Henan Engineering Research Center for Biochar, Zhengzhou 450002, Henan, China
    2Dali Municipal Branch of Yunnan Tobacco Company, Dali 671000, Yunnan, China
    3Nanping Municipal Branch of Fujian Tobacco Company, Nanping 353100, Fujian, China
    4Jiyuan Municipal Branch of Henan Tobacco Company, Jiyuan 454650, Henan, China
    5College of Forestry, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2021-08-25 Revised:2021-10-09 Online:2022-04-15 Published:2022-04-24
  • Contact: Ren Tianbao,Yang Xitian

RichHTML

7

PDF (PC)

252

摘要：

以烤烟品种K326为供试材料开展土培盆栽试验,在人工气候室条件下设置3种光照强度,研究叶面喷施钙素对不同光照强度下烤烟叶片光合色素、气体交换参数、抗氧化酶活性、渗透调节物质及干物质积累的影响,探明外源钙对光胁迫条件下烤烟光合生理特性及抗氧化系统的调控作用。结果表明,喷施外源钙可以增加不同光照强度下烤烟叶片的叶绿素（Chla+b）及类胡萝卜素（Car）含量,Chla+b和Car含量的最高值分别出现在400~500μmol/(m2·s)低光（L1）和1500~1800μmol/(m2·s)高光（L3）处理。烤烟叶片的净光合速率（Pn）随光强增加而升高,但光能利用效率（LUE）以L1处理最高。增施外源钙后烤烟叶片的Pn、蒸腾速率（Tr）、气孔导度（Gs）以及LUE显著增加,且在L1与L3处理下LUE增加比例较高,分别比对照增加了3.83%~5.63%和4.65%~7.75%。高光喷钙处理的超氧化物歧化酶活性、过氧化物酶活性、可溶性糖含量以及干物质积累量最高,丙二醛含量最低。喷施外源钙可提高烟草叶片活性氧清除酶活性和渗透调节物质含量来保护光胁迫条件下光系统Ⅱ（PSⅡ）反应中心不受伤害,促进光合效率提高,有利于光合碳同化产物的积累。

关键词: 钙, 光胁迫, 烤烟, 光合生理特性

Abstract:

The effects of the exogenous calcium on the photosynthetic pigments, gas exchange parameters, antioxidant enzyme activities, osmoregulation substances and dry matter accumulation of flue-cured tobacco were investigated with flue-cured tobacco variety K326 as experimental material. The soil-culture pot experiment was carried out under three light intensities in the artificial climate chamber in order to study the regulating effects of the exogenous calcium salts on the photosynthetic physiological characteristics and antioxidant system of flue-cured tobacco under light stress. The results showed that the contents of chlorophyll (Chla+b) and carotenoid (Car) in flue-cured tobacco leaves under different light intensities were increased by the application of exogenous calcium, and the highest values of Chla+b and Car respectively appeared in 400-500μmol/(m2·s) low light treatment (L1) and 1500-1800μmol/(m2·s) high light treatment (L3). With the increase of light intensity, the net photosynthetic rate (Pn) was increased, but the light energy utilization efficiency (LUE) of L1 treatment was the highest. The values of Pn, transpiration rate (Tr), stomatal conductance (Gs) and LUE were significantly increased by spraying exogenous calcium on flue-cured tobacco leaves,the increase proportion of LUE was higher under L1 and L3 treatments, increased by 3.83%-5.63% and 4.65%-7.75% compared with the control, respectively. The superoxide dismutase and peroxidase activities, the soluble sugar and dry matter accumulation contents were the highest under the condition of L3 treatment with exogenous calcium application, but the content of malonaldehyde was the lowest. Consequently, in order to protect the PSII reaction center under light stress, the exogenous calcium application enhanced the active oxygen scavenging enzyme activity and osmotic adjustment substance content, the photosynthetic efficiency of flue-cured tobacco could be effectively improved so that the photosynthetic carbon assimilation products could be promoted.

Key words: Calcium, Light stress, Flue-cured tobacco (Nicotinna tobacum L.), Photosynthesis physiology characteristics

图1

叶面喷施钙素对光胁迫条件下烤烟叶片光合色素含量的影响 不同小写字母分别表示不同光照强度（括号外）和钙盐处理间（括号内）在P<0.05水平差异显著,下同

图2

叶面喷施钙素对光胁迫条件下烤烟叶片气体交换参数的影响

图3

叶面喷施钙素对光胁迫条件下烤烟LUE的影响

图4

叶面喷施钙素对光胁迫条件下烤烟叶片抗氧化酶活性的影响

图5

叶面喷施钙素对光胁迫条件下烤烟叶片MDA及SS含量的影响

图6

叶面喷施钙素对光胁迫条件下烤烟干物质积累量的影响

[1] Chen L Y, Tarin M W K, Huo H Q, et al. Photosynthetic responses of Anthurium ‘Red’ under different light conditions. Plants, 2021, 10(5):857.
doi: 10.3390/plants10050857
[2] 魏明月, 云菲, 刘国顺, 等. 不同光环境下烟草光合特性及同化产物的积累与分配机制. 应用生态学报, 2017, 28(1):159-168.
[3] Shafiq I, Hussain S, Raza M A, et al. Crop photosynthetic response to light quality and light intensity. Journal of Integrative Agriculture, 2021, 20(1):4-23.
doi: 10.1016/S2095-3119(20)63227-0
[4] Tan W, Meng Q W, Bresticb M, et al. Photosynthesis is improved by exogenous calcium in heat-stressed tobacco plants. Journal of Plant Physiology, 2011, 168:2063-2071.
doi: 10.1016/j.jplph.2011.06.009
[5] 张会慧, 包卓, 许楠, 等. 钙对低温高光锻炼下烤烟幼苗光合的促进效应. 核农学报, 2011, 25(3):588-587.
[6] 张燕, 方力, 李天飞, 等. 钙对烟草叶片热激忍耐和活性氧代谢的影响. 植物学通报, 2002, 19(6):721-726.
[7] 云菲, 刘国顺, 杨永峰, 等. 外源钙盐对两种基因型烤烟光合作用的调控效应. 植物生理学报, 2013, 49 (12):1375-1384.
[8] 郑远, 陈兆进. 植物细胞器钙信号研究进展. 植物生理学报, 2015, 51(8):1195-1203.
[9] Liu Y F, Zhang G X, Qi M F, et al. Effects of calcium on Photosynthesis,antioxidant system,and chloroplast ultrastructure in tomato leaves under low night temperature stress. Journal of Plant Growth Regulation, 2015, 34:263-273.
doi: 10.1007/s00344-014-9462-9
[10] 魏明月, 云菲, 刘国顺, 等. CaCl2对强光胁迫下烤烟光合特性的影响. 中国烟草学报, 2016, 22(3):37-43.
[11] Fan Y F, Chen J X, Wang Z L, et al. Soybean (Glycine max L. Merr.) seedlings response to shading:leaf structure,photosynthesis and proteomic analysis. BMC Plant Biology, 2019, 19(1):34.
doi: 10.1186/s12870-019-1633-1
[12] 徐臣善. 钙对盐胁迫下小金海棠幼苗生物量及抗氧化系统的影响. 植物生理学报, 2014, 50(6):817-822.
[13] Naeem M, Ahmad R, Ihsan M Z, et al. Foliar calcium spray confers drought stress tolerance in maize via modulation of plant growth,water relations,proline content and hydrogen peroxide activity. Archives of Agronomy and Soil Science, 2018, 64:116-131.
doi: 10.1080/03650340.2017.1327713
[14] 张国显. 外源钙缓解低夜温导致番茄叶片光抑制的机理. 沈阳:沈阳农业大学, 2015.
[15] Wu D, Liu Y F, Pang J Y, et al. Exogenous calcium alleviates nocturnal chilling-induced feedback inhibition of photosynthesis by improving sink demand in peanut (Arachis hypogaea). Frontiers in Plant Science, 2020, 11:607029.
doi: 10.3389/fpls.2020.607029
[16] 任城帅, 李慧, 翁小航, 等. 外源钙对水曲柳生长、光合特性及水分利用效率的影响. 沈阳农业大学学报, 2020, 51(6):663-669.
[17] 李合生, 孙群, 赵世杰, 等. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
[18] 王嘉佳, 唐中华. 可溶性糖对植物生长发育调控作用的研究进展. 植物学研究, 2014(3):71-76.
[19] 云菲, 宋晶, 郭二辉, 等. 不同光照条件下外源钙对“烟株―土壤”系统中光合碳分配的影响. 中国烟草学报, 2019, 25(6):27-37.
[20] 李贺, 刘世琦, 陈祥伟, 等. 钙对水培青蒜苗生长、光合特性及品质的影响. 植物营养与肥料学报, 2013, 19(5):1118-1128.
[21] 周君, 肖伟, 陈修德, 等. 外源钙对‘黄金梨’叶片光合特性及果实品质的影响. 植物生理学报, 2018, 54(3):449-455.
[22] 杜保伟, 方庆, 胡月华, 等. 叶面喷钙对‘3-18’桃叶片性状的影响. 北方园艺, 2014,(24):21-24.
[23] 程亚娇, 范元芳, 谌俊旭, 等. 光照强度对大豆叶片光合特性及同化物的影响. 作物学报, 2018, 44(12):1867-1874.
[24] Hochmal A K, Schulze S, Trompelt K, et al. Calcium-dependent regulation of photosynththesis. Biochimica ET Biophysica Acta-Biomembranes, 2015, 1847:993-1003.
[25] Petroutsos D, Busch A, Janssen I, et al. The chloroplast calcium sensor CAS is required for photoacclimation in Chlamydomonas reinhardtii. Plant Cell, 2011, 23:2950-2963.
doi: 10.1105/tpc.111.087973
[26] Schwartz A, Ilan N, Grantz D A. Calcium effects on stomatal movements in Commelina communis L.:use of EGTA to modulate stomatal response to light,KCl and CO2. Plant Physiology, 1988, 87(3):583-587.
doi: 10.1104/pp.87.3.583 pmid: 16666189
[27] 齐明芳, 刘玉凤, 周龙发, 等. 钙对亚高温下番茄幼苗叶片光合作用的调控作用. 中国农业科学, 2011, 44(3):531-537.
[28] Hou J F, Li J, Yang Y, et al. Physiological and transcriptomic analyses elucidate that exogenous calcium can relieve injuries to potato plants (Solanum tuberosum L.) under weak light. International Journal of Molecular Sciences, 2019, 20(20):5133.
doi: 10.3390/ijms20205133
[29] 李利红. 水杨酸、Ca2+和NO对高温强光胁迫下小麦叶绿体D1蛋白和PSII功能的调节作用. 郑州:河南农业大学, 2010.
[30] 秦舒浩, 张俊莲, 孔令娟, 等. 高温强光下Ca2+对西葫芦幼苗膜质过氧化、抗氧化酶系统及热耗散的影响. 中国生态农业, 2012, 20(3):343-347.
[31] 张燕, 李天飞, 方力, 等. 钙对高温胁迫下烟草幼苗抗氧化代谢的影响. 生命科学研究, 2002, 6(4):356-361.
[32] 杨亚军.外源Ca2+对高温强光胁迫下小麦叶绿体D1蛋白周转及光合作用的影响. 郑州:河南农业大学, 2009.
[33] 王芳, 杨莎, 郭峰, 等. 钙对花生幼苗生长、活性氧积累和光抑制程度的影响. 生态学报, 2015, 35(5):1496-1504.
[1] 何宇轩, 李雅娟, 周明卓, 眭锋, 吕伟生, 张俊, 曾勇军, 黄山. 秸秆全量还田下施用过氧化钙对南方双季稻产量和稻田温室气体排放的影响[J]. 作物杂志, 2022, (1): 116–123
[2] 谷宇超, 杨懿德, 鄢敏, 刘勇, 杨建, 向金友, 罗柱石, 李林秋, 景延秋, 杨洋. 打顶后喷施不同浓度GA3和6-BA对烤烟农艺性状和化学成分的影响[J]. 作物杂志, 2021, (6): 171–176
[3] 王初亮, 宋文峰, 关罗浩, 谢晋, 黄浩, 李旺阳, 王维. 云南红河烟区覆膜方式及移栽苗龄对烤烟产量和品质形成的影响[J]. 作物杂志, 2021, (6): 95–100
[4] 刘文龙, 宁尚辉, 曹明锋, 祝利, 高玉珍, 张学伟, 文梓祥, 姜宝迪, 景延秋, 邓勇. 桃源县植烟土壤微量元素与烟叶常规化学成分相关性分析[J]. 作物杂志, 2021, (5): 176–180
[5] 张久权, 余祥文, 凌爱芬, 王勇, 李磊磊, 董建新. 烤烟膜下小苗移栽育苗盘规格优选研究[J]. 作物杂志, 2021, (4): 123–129
[6] 于天一, 郑亚萍, 邱少芬, 姜大奇, 吴正锋, 郑永美, 孙学武, 沈浦, 王才斌, 张建成. 酸化土壤施钙对不同花生品种（系）钙吸收、利用及产量的影响[J]. 作物杂志, 2021, (4): 80–85
[7] 汪坤, 魏跃伟, 姬小明, 云菲, 邹凯, 隆准. 生物炭基肥与哈茨木霉菌剂配施对烤烟和植烟土壤质量的影响[J]. 作物杂志, 2021, (3): 106–113
[8] 王慧芳, 张希, 冯小虎, 李一凡, 张红, 赵松超, 赵铭钦. 不同植物生长调节剂对烤烟生长发育的影响[J]. 作物杂志, 2021, (3): 173–177
[9] 冯新维, 黄莺, 吴贵丽, 芶剑渝, 彭玉龙. 不同钙浓度对烤烟生长及镁吸收的影响[J]. 作物杂志, 2021, (3): 190–194
[10] 张希, 王慧芳, 代卓毅, 薛刚, 徐世晓, 杨铁钊. 基因型、施氮量及其互作对烤烟多酚类物质的影响[J]. 作物杂志, 2021, (3): 84–90
[11] 张芸萍, 郭山虎, 张锦涛, 解燕, 易克, 李强. 曲靖烟区烟叶微量元素与土壤pH值关系研究[J]. 作物杂志, 2021, (2): 178–182
[12] 王德权, 孙延国, 杜玉海, 刘洋, 王艺, 马兴华, 张玉琴, 张日强. 移栽时间与方式对烤烟生长发育及产量、品质的影响[J]. 作物杂志, 2021, (2): 87–95
[13] 黄丹, 杨富文, 刘琳, 王月, 贺国强, 王洪瑞, 孙广玉, 敖红. 氮及氮素形态对烤烟叶片叶绿素光合荧光参数的影响[J]. 作物杂志, 2021, (1): 150–159
[14] 周江, 谢宜章, 向平安. 湖南主要大田作物系统投入产出的能值分析[J]. 作物杂志, 2021, (1): 175–181
[15] 李迪秦, 王青, 胡亚杰, 王艳, 王妍妮, 钟仪, 刘铭晖, 丁春霞. 湘烟5号对植烟土壤镉耐受力响应研究[J]. 作物杂志, 2021, (1): 182–186
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!