Crops ›› 2016, Vol. 32 ›› Issue (6): 154-159.doi: 10.16035/j.issn.1001-7283.2016.06.026

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Effects of Arc-Tooth-Shaped Corona Discharge Field Treatment on Improving Corn Seed Vigor and Physiological Indexes

Yang Cuicui1,Li Fade2,Li Yan1,Wu Chenglai1,Zhang Chunqing1   

  1. 1 State Key Laboratory of Crop Biology,Agronomy college,Shandong Agricultural University,Taian 271018,Shandong,China
    2 Mechanical and Electrical Engineering College,Shandong Agricultural University,Taian 271018,Shandong,China
  • Received:2016-09-02 Revised:2016-11-01 Online:2016-12-15 Published:2018-08-26
  • Contact: Chunqing Zhang

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Abstract:

To understand mechanism of arc-tooth-shaped corona discharge field (ATSCDF) on improving corn seed vigor, three samples of two hybrids, Nongda 108 and Jinbei 288, were used to study influences of seed moisture content and seed vigor after ATSCDF treatment. The optimized electric field strength and treatment time changed with seed moisture content. The longer treatment time and the greater varied the field strength became. Percentage of seed germination of middle and low vigor seeds could be increased by from 12% to 24%, of which vigor index was increased by from 28%-51%. The optimal treatment condition of ATSCDF was about 350 kV/m and 5.6min under normal moisture content. The six physiological indexes of seeds treated under optimal conditions, which were the electrical conductivity, the activities of amylase, dehydrogenase, superoxide dismutase (SOD), peroxidase(POD) and catalase(CAT), were tested and compared with control. The activities of amylase, dehydrogenase, SOD, POD, and CAT increased significantly compared with the control for seeds of different vigor, and the electrical conductivity decreased significantly to the control. It was cleared that seed vigor could be actively increased by the treatment of ATSCDF under optimal condition, because of improvement of activities of many kinds of metabolic enzymes and protective enzymes, and of capability of repairing cell membrane.

Key words: Corn seed, Arc-tooth-shaped corona discharge field (ATSCDF), Seed moisture content, Seed vigor, Physiological indexes

Fig.1

Schematic of seed high-voltage electric field processing1.AC transformer,2.High-voltage silicon stack,3.Arc electrodes,4.Bottom plate,5.Corn seeds,6.Ammeter,7.High-voltage meter"

Table 1

Factors and levels of experiment"

水平
Level		 因素Factor
场强Field strength(kV/m) 时间Time(min)
6.0% 12.5% 16.0% 6.0% 12.5% 16.0%
-1.414 285 280 160 5.6 4.8 3.6
-1.000 320 300 200 6.0 5.0 4.0
0.000 400 350 300 7.0 5.5 5.0
1.000 480 400 400 8.0 6.0 6.0
1.414 515 420 440 8.4 6.2 6.4

Table 2

Comparison of vigor index on maize seeds of three moisture content"

指标Index 处理
Treatment		 种子含水量
Seed moisture content
6.0% 12.5% 16.0%
发芽势(%)
Germination potential		 CK 64 54 67
优化条件处理 79** 69** 77**
相对提高(%) 23.4 27.8 14.9
发芽率(%)
Germination rate		 CK 74 74 74
优化条件处理 85** 84** 84**
相对提高(%) 14.9 13.5 13.5
发芽指数
Germination index		 CK 9.1 8.8 9.2
优化条件处理 10.6** 10.1** 10.4**
相对提高(%) 16.9 15.3 13.5
活力指数Vigor index CK 5.11 4.85 5.71
优化条件处理 6.89** 6.26** 7.23**
相对提高(%) 34.8 29.1 26.7
综合活力指标
Comprehensive vigor index		 CK 7.88 7.54 8.16
优化条件处理 9.69** 9.05** 9.64**
相对提高(%) 23.0 20.0 18.1

Fig.2

Comparison of four vigor index with three kinds of vigor maize seeds Different capital letters and small letters mean significant difference at 0.01 and 0.05 level, respectively, the same below"

Fig.3

Comparison of electric conductivity on three kinds of maize seed with different vigor"

Fig.4

Comparison of α-amylase and dehydrogenase activity on three kinds of maize seed with different vigor"

Fig.5

Comparison of SOD, POD, and CAT activity on three kinds of maize seed with different vigor"

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