Crops ›› 2017, Vol. 33 ›› Issue (1): 83-87.doi: 10.16035/j.issn.1001-7283.2017.01.016

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Physiological and Biochemical Characteristics and Root Morphology of Fagopyrum megaspartanium under Hydroponic Culture

Wang Hongxin1,Yuan Yi2,Yang Sudan3,Liu Hongmei3   

  1. 1Sanya College,Sanya 572000,Hainan,China
    2Jiangxi Institute of Fashion Technology,Nanchang 330201,Jiangxi,China
    3Chongqing Vocational Institute of Tourism,Chongqing 409000,China
  • Received:2016-11-18 Revised:2016-12-10 Online:2017-02-15 Published:2018-08-26

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Abstract:

In order to study the adaptation of Fagopyrum megaspartanium in hydroponic culture providing theoretical foundation for its hydroponic-culture, a hydroponic experiment was conducted with three different NAA levels (0.27, 0.54, 1.08mmol/L) to determinate the root growth of F.megaspartanium after 10d and 20d. Total length, surface area and volume of roots were calculated to obtain suitable NAA level for root growth of F.megaspartanium. The branches of F.megaspartanium were treated through the optimal NAA level and put into water to induce adventitious roots. The branches were divided into two groups to be cultured in nutrient solution and soil respectively. The root morphology and physiological indexes were determined after 40d of culture．Results showed that the optimal inducing concentration of NAA promoting F.megaspartanium rooting of water root was 0.27mmol/L; Compared with the short brown hairy roots of the soil cultivated plants, the hydroponic roots were white or light green with secondary roots, but without root hairs. Roots from the hydroponic culture had more fresh weight, root/shoot ratio, total root length, surface area, volume and antioxidant enzymes activities than those from the soil culture. The contents of chlorophyll a,chlorophyll-b,total chlorophyll and carotenoid in hydroponic group were lower than that in soil cultured group except for water content and chlorophyll a/b. The activities of POD, SOD in roots were higher than that in leaves of hydroponic and cultured group ( P<0.05) . The activities of POD, SOD in roots of hydroponic group were higher than that in roots of soil cultured group ( P<0.05) ,and the activity of CAT and the MDA content showed no significant difference. Hydroponic could influence root morphological characteristics, pigment contents and some of the capacity of antioxidant, however part of the morphological, and physiological and biochemical indexes were changed to adapt hydroponic environment.

Key words: Hydroponic culture, Fagopyrum megaspartanium, Root morphology, Anti-oxidant enzymes, Nitrate reductase, Malondialdehyde

Table 1

The root system morphological characteristics of F.megaspartanium under different concentrations of NAA"

NAA
(mmol/L)		 总根长Total root length(cm) 根表面积Root surface area(cm2) 根体积Root volume(cm3)
10d 20d 10d 20d 10d 20d
0(CK) 273.44±9.22b 343.37±2.70d 52.00±2.39d 68.91±1.53c 0.38±0.31c 0.45±0.03d
0.27 321.11±5.79a 418.36±4.95a 66.30±1.01a 89.19±1.00a 0.53±0.40a 0.62±0.04a
0.54 309.97±3.97a 408.63±2.18b 61.89±1.51b 86.52±3.05a 0.49±0.15b 0.59±0.02b
1.08 281.89±9.46b 336.21±5.84c 58.53±1.45c 74.37±1.20b 0.41±0.06c 0.51±0.03c

Fig.1

The contrast of root morphology between hydroponic and soil cultured F.megaspartanium"

Table 2

The root system morphological characteristics of F.megaspartanium under different culture condition"

处理
Treatment		 根系鲜重(g)
Root fresh weight		 根冠比(%)
Root/shoot ratio		 总根长(cm)
Total root length		 根表面积(cm2)
Root surface area		 根体积(cm3)
Root volume		 根系活力[g/(g·h)]
Root activity
水培Hydroponics culture 0.796±0.035a 0.082±0.0036a 617.03±7.55a 100.66±5.2a 0.75±0.05a 0.584±0.200a
土培Soil culture 0.619±0.027b 0.067±0.0046b 558.23±6.50b 83.46±4.54b 0.63±0.03b 0.497±0.045b

Table 3

The photosynthetic pigment contents and water content in fronds of F.megaspartanium under different culture condition"

处理Treatment 叶绿素a(mg/g)
Chlorophyll a		 叶绿素b(mg/g)
Chlorophyll b		 叶绿素a/b
Chlorophyll a/b		 总叶绿素(mg/g)
Total chlorophyll		 类胡萝卜素(mg/g)
Carotenoid		 水分(%)
Water
水培Hydroponics culture 1.65±0.03b 0.39±0.02b 4.28±0.04a 2.81±0.02b 0.27±0.02b 88.65±3.21a
土培Soil culture 2.33±0.02a 0.76±0.01a 3.07±0.03b 3.61±0.04a 0.33±0.02a 75.15±3.16b

Table 4

The physiological and biochemical characteristics of F.megaspartanium under different culture condition"

处理Treatment POD[U/(g·min)] SOD[U/(g·min)] CAT[U/(g·min)] NR[U/(g·min)] MDA(nmol/mg)
水培叶Leaf of hydroponics culture 147.95±3.05c 89.83±1.31c 97.22±1.00b 42.61±0.56c 3.35±0.02b
土培叶Leaf of soil culture 126.33±3.05d 76.89±2.29d 95.60±0.56b 42.56±0.58c 3.14±0.06b
水培根Root of hydroponics culture 416.65±4.51a 113.80±1.47a 148.36±2.81a 338.60±2.82a 5.09±0.91a
土培根Root of soil culture 368.73±2.52b 96.49±2.80b 145.90±2.43a 112.66±2.60b 4.87±0.18a
[1] Li A R, Hong S P. Flora of China. Beijing: Science Press, 2004: 132-135.
[2] 何显忠 . 金荞麦的药理作用和临床应用. 时珍国医国药, 2001,12(4):316-317.
[3] 艾群, 王斌, 王国清 . 金荞麦制剂的抑菌研究. 黑龙江医学, 2002,26(9):666.
[4] 吴清, 梁国鲁 . 金荞麦野生资源的开发与利用. 中国野生植物资源, 2001,20(2):27-28.
[5] 赵丽丽, 邓蓉, 向清华 , 等. 高温对金荞麦光合气体交换和叶绿素荧光特性的影响. 草业科学, 2015,32(4):560-569.
doi: 10.11829\j.issn.1001-0629.2014-0353
[6] 王雯, 张益锋 . 干旱及增强UV-B胁迫对金荞麦光合色素的影响. 安徽农业科学, 2012,40(11):6463-6467.
[7] 何鑫, 张存政, 刘贤金 , 等. 外源硝酸钙对水培生菜生长及矿质元素吸收的影响. 核农学报, 2016,30(12):2460-2466.
[8] 秦成, 裴红宾, 张永清 , 等. 磷素对小豆幼苗生长发育的影响.作物杂志, 2015(3):122-129.
[9] 邓衍明, 叶晓青, 梁丽建 , 等. 不同瓣型茉莉水培生根能力及根尖解剖结构比较. 植物资源与环境学报, 2014,23(1):9-15.
[10] 徐开杰, 史丽丽, 王勇锋 , 等. 水培条件下pH值对柳枝稷幼苗生长发育的影响. 生态学报, 2015,35(23):7690-7698.
doi: 10.5846/stxb201405040877
[11] 张志良, 瞿伟菁 . 植物生理学试验指导.3版.北京: 高等教育出版, 2003.
[12] 李合生 . 植物生理生化试验原理和技术.北京: 高等教育出版社, 2000.
[13] 张景云, 白雅梅, 缪南生 , 等. 盐胁迫对不同耐盐性二倍体马铃薯叶片质膜透性、丙二醛和脯氨酸含量的影响.作物杂志, 2013(4):75-79.
[14] Kyaing M S, 顾立江,程红梅.植物中硝酸还原酶和亚硝酸还原酶的作用. 生物技术进展, 2011,1(3):159-164.
[15] 原红娟, 王福生 . 白鹤芋水培与土培根显微系结构比较.江苏农业科学, 2010(3):244-245.
[16] 马雪泷, 吴杰, 邹鹏飞 , 等. 水培与土培栀子显微结构及生理指标的比较. 东北林业大学学报, 2011,39(7):19-21.
[17] 邸保, 陈段芬, 方正 , 等. 新几内亚凤仙水培与土培根系特征的比较. 中国农学通报, 2012,28(1):162-165.
[18] 孔妤, 王忠, 顾蕴洁 , 等. 土培和水培吊兰根系结构的观察. 园艺学报, 2009,36(4):533-538.
[19] 王立德, 廖红, 王秀荣 , 等. 植物根毛的发生、发育及养分吸收. 植物学通报, 2004,21(6):649-659.
[20] 陈海生 . 营养液膜条件下樱桃番茄生长和生理特性研究.农机化研究, 2011(10):123-125.
[21] 孟佳丽, 王夏雯, 余翔 , 等. 不同生根剂处理对水培密叶朱蕉根系的影响. 江苏农业科学, 2014,42(1):142-144.
[22] Zhang Y F, Li W L, Meng W F . A comparative study on the leaf characteristics and root vigor of bowl lotus under hydroponics and soil culture. Agricultural Science & Technology, 2013,14(9):1267-1270.
[23] 李奕奉, 闫冬, 何映雪 , 等. 在水培方式下锶对白菜和油菜某些生理指标的影响. 癌变·畸变·突变, 2016,28(3):223-226.
[24] 卢鹏, 龚一富 . 水培对中国红豆杉(Taxus chinensis)根系结构及生理生化的影响. 生物学杂志, 2015,32(1):67-71.
[25] 顿颖 . 柑橘水培体系优化及N、Fe胁迫下根系形态研究. 武汉:华中农业大学, 2011.
[26] 王智威, 牟思维, 闫丽丽 , 等. 水分胁迫对春播玉米苗期生长及其生理生化特性的影响. 西北植物学报, 2013,33(3):343-351.
[27] 田华, 段美洋, 王兰 . 植物硝酸还原酶功能的研究进展. 中国农学通报, 2009,25(10):96-99.
[28] 潘瑞炽, 王小菁, 李娘辉 . 植物生理学.6版.北京: 高等教育出版社, 2008: 45.
[29] 华春, 王仁雷 . 杂交稻及其三系叶片衰老过程中SOD、CAT活性和MDA含量的变化. 西北植物学报, 2003,23(3):406-409.
[1] . [J]. Crops, 2013, 29(3): 53-55.
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