Crops ›› 2017, Vol. 33 ›› Issue (1): 83-87.doi: 10.16035/j.issn.1001-7283.2017.01.016

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Physiological and Biochemical Characteristics and Root Morphology of Fagopyrum megaspartanium under Hydroponic Culture

Wang Hongxin1,Yuan Yi2,Yang Sudan3,Liu Hongmei3   

  1. 1Sanya College,Sanya 572000,Hainan,China
    2Jiangxi Institute of Fashion Technology,Nanchang 330201,Jiangxi,China
    3Chongqing Vocational Institute of Tourism,Chongqing 409000,China
  • Received:2016-11-18 Revised:2016-12-10 Online:2017-02-15 Published:2018-08-26

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Abstract:

In order to study the adaptation of Fagopyrum megaspartanium in hydroponic culture providing theoretical foundation for its hydroponic-culture, a hydroponic experiment was conducted with three different NAA levels (0.27, 0.54, 1.08mmol/L) to determinate the root growth of F.megaspartanium after 10d and 20d. Total length, surface area and volume of roots were calculated to obtain suitable NAA level for root growth of F.megaspartanium. The branches of F.megaspartanium were treated through the optimal NAA level and put into water to induce adventitious roots. The branches were divided into two groups to be cultured in nutrient solution and soil respectively. The root morphology and physiological indexes were determined after 40d of culture．Results showed that the optimal inducing concentration of NAA promoting F.megaspartanium rooting of water root was 0.27mmol/L; Compared with the short brown hairy roots of the soil cultivated plants, the hydroponic roots were white or light green with secondary roots, but without root hairs. Roots from the hydroponic culture had more fresh weight, root/shoot ratio, total root length, surface area, volume and antioxidant enzymes activities than those from the soil culture. The contents of chlorophyll a,chlorophyll-b,total chlorophyll and carotenoid in hydroponic group were lower than that in soil cultured group except for water content and chlorophyll a/b. The activities of POD, SOD in roots were higher than that in leaves of hydroponic and cultured group ( P<0.05) . The activities of POD, SOD in roots of hydroponic group were higher than that in roots of soil cultured group ( P<0.05) ,and the activity of CAT and the MDA content showed no significant difference. Hydroponic could influence root morphological characteristics, pigment contents and some of the capacity of antioxidant, however part of the morphological, and physiological and biochemical indexes were changed to adapt hydroponic environment.

Key words: Hydroponic culture, Fagopyrum megaspartanium, Root morphology, Anti-oxidant enzymes, Nitrate reductase, Malondialdehyde

Table 1

The root system morphological characteristics of F.megaspartanium under different concentrations of NAA"

NAA
(mmol/L)		 总根长Total root length(cm) 根表面积Root surface area(cm2) 根体积Root volume(cm3)
10d 20d 10d 20d 10d 20d
0(CK) 273.44±9.22b 343.37±2.70d 52.00±2.39d 68.91±1.53c 0.38±0.31c 0.45±0.03d
0.27 321.11±5.79a 418.36±4.95a 66.30±1.01a 89.19±1.00a 0.53±0.40a 0.62±0.04a
0.54 309.97±3.97a 408.63±2.18b 61.89±1.51b 86.52±3.05a 0.49±0.15b 0.59±0.02b
1.08 281.89±9.46b 336.21±5.84c 58.53±1.45c 74.37±1.20b 0.41±0.06c 0.51±0.03c

Fig.1

The contrast of root morphology between hydroponic and soil cultured F.megaspartanium"

Table 2

The root system morphological characteristics of F.megaspartanium under different culture condition"

处理
Treatment		 根系鲜重(g)
Root fresh weight		 根冠比(%)
Root/shoot ratio		 总根长(cm)
Total root length		 根表面积(cm2)
Root surface area		 根体积(cm3)
Root volume		 根系活力[g/(g·h)]
Root activity
水培Hydroponics culture 0.796±0.035a 0.082±0.0036a 617.03±7.55a 100.66±5.2a 0.75±0.05a 0.584±0.200a
土培Soil culture 0.619±0.027b 0.067±0.0046b 558.23±6.50b 83.46±4.54b 0.63±0.03b 0.497±0.045b

Table 3

The photosynthetic pigment contents and water content in fronds of F.megaspartanium under different culture condition"

处理Treatment 叶绿素a(mg/g)
Chlorophyll a		 叶绿素b(mg/g)
Chlorophyll b		 叶绿素a/b
Chlorophyll a/b		 总叶绿素(mg/g)
Total chlorophyll		 类胡萝卜素(mg/g)
Carotenoid		 水分(%)
Water
水培Hydroponics culture 1.65±0.03b 0.39±0.02b 4.28±0.04a 2.81±0.02b 0.27±0.02b 88.65±3.21a
土培Soil culture 2.33±0.02a 0.76±0.01a 3.07±0.03b 3.61±0.04a 0.33±0.02a 75.15±3.16b

Table 4

The physiological and biochemical characteristics of F.megaspartanium under different culture condition"

处理Treatment POD[U/(g·min)] SOD[U/(g·min)] CAT[U/(g·min)] NR[U/(g·min)] MDA(nmol/mg)
水培叶Leaf of hydroponics culture 147.95±3.05c 89.83±1.31c 97.22±1.00b 42.61±0.56c 3.35±0.02b
土培叶Leaf of soil culture 126.33±3.05d 76.89±2.29d 95.60±0.56b 42.56±0.58c 3.14±0.06b
水培根Root of hydroponics culture 416.65±4.51a 113.80±1.47a 148.36±2.81a 338.60±2.82a 5.09±0.91a
土培根Root of soil culture 368.73±2.52b 96.49±2.80b 145.90±2.43a 112.66±2.60b 4.87±0.18a
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