Crops ›› 2018, Vol. 34 ›› Issue (5): 17-20.doi: 10.16035/j.issn.1001-7283.2018.05.003

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Analysis of Peroxidase Zymogram and Genetic Effects between Rice Lines and Their Parents During Grain Filling

Ji Shengdong1,Li Peng1,Li Jiangwei2,Song Liumin1,Liu Miaomiao1,Gao Kuanglong1,Yin Haiqing3   

  1. 1 College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China
    2 Henan Xinxiang Academy of Agricultural Sciences, Xinxiang 453000, Henan, China
    3 Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2018-03-08 Revised:2018-08-27 Online:2018-10-15 Published:2018-10-12
  • Contact: Haiqing Yin

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Abstract:

The dynamic changes of peroxidase zymogram of the C and their parents at the grain filling stage were analyzed by renaturation electrophoresis. The results showed as follows: (1) The C showed a different type of new peroxidase zymogram than parents in grain filling stage, the C band type partial A and the enzyme activity changes was similar to the B. (2) The 52kD new band was detected in the mid-filling stage of C. The 53kD band was detected only in the late filling stage of the A and C, and enzyme activity of the C was weaker than that of the A. (3) 70~63kD in A, 63~58kD and 42kD in the B, both of them didn’t detect in all filling stage in C. Peroxidase zymogram differences in C resulting from parental gene interaction were expected from breeding. Further study on the relationship between the new band and the differential zymogram and the new progeny traits would provide important theoretical basis for hybrid rice breeding.

Key words: Peroxidase, Genetic effects, Renaturation electrophoresis, Rice, Parents

Table 1

Comparison of differences between parents and offspring of rice"

材料
Material		 株高(cm)
Plant height		 剑叶长(cm)
Length of
flag leaf		 穗长(cm)
Ear length		 单株有效分蘖/单株总分蘖
Effective tillers per plant/
Total tillers per plant		 穗总粒数
Grains per
panicle		 穗实粒数
Solid grains
per panicle		 千粒重(g)
1000-seed weight		 米质
Rice
quality		 生育期(d)
Growing
period		 穗颈瘟
Rice blast disease
A 105 36 23 17/20 175 163 26.11 等外 153 感
B 96 25 15 10/12 122 111 27.2 优2 165 中抗
C 98 26 19 13/15 125 113 26.27 优3 161 中抗

Fig.1

The leaf POD zymogram of rice offspring and parents in grain filling stage A, Female parent; B, Male parent; C, Offspring; The number 1, 2 and 3 were respectively in the early, middle and late stage of grain filling; M was the standard protein marker. The same below"

Fig.2

The leaf total POD activity of rice offspring and parents in grain filling stage"

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