Crops ›› 2019, Vol. 35 ›› Issue (1): 68-74.doi: 10.16035/j.issn.1001-7283.2019.01.011

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Screening of High Aroma Mutants from Progenies of EMS Mutagenized Flue-Cured Tobacco

Xinling Yang1,Qian Yao1,Wenli Ping2,Yiqiong Ma1,Baolin Wang1,Guotao Jia1,Yongfeng Yang1,Hong Cui3   

  1. 1 Technology Centre, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450000, Henan, China
    2 Institute of Tobacco Research, HAAS/Key Laboratory for Green Preservation & Control of Tobacco Disease & Pests in Huanghuai Tobacco Growing Area, Xuchang 460001, Henan, China;
    3 College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2018-09-12 Revised:2018-11-21 Online:2019-02-15 Published:2019-02-01
  • Contact: Yongfeng Yang

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Abstract:

In order to screen the high-aroma and high-quality flue-cured tobacco offspring, Zhongyan 100 was used as the experimental material, and EMS mutagenesis technology was employed to treat the Zhongyan 100 seed and screen out two mutations with high density of leaf glandular trichomes. The body material was evaluated by field test for its glandular trichomes density, chemical composition of the leaf surface, chemical composition after baking, aroma substance content and sensory quality evaluation, and compared with the normal tobacco Zhongyan 100 offspring and NC89. The results showed that the density of glandular trichomes of EMS-2 mutagenized strains was 1.8 times and 1.2 times that of Zhongyan 100 and NC89, respectively. The chemical composition analysis of leaf surface showed that EMS-1 mutagenized strains the contents of cembranes and alkanes were the highest. The content of the sucrose esters in the EMS-2 mutant strain was the most abundant. The chemical composition analysis of the EMS-2 mutant strain showed that the chemical components of the EMS-2 mutant strain were more harmonious, and the aroma components were the highest. Compared with Zhongyan 100 and NC89, it increased by 34.5% and 29.0%. Combined with the sensory evaluation of mutagenized offspring, it was concluded that the comprehensive quality of mutagenic strains was better, and the quality of tobacco leaves could be improved. The comprehensive quality of EMS-2 was the best and could be used as selected strains proceed to the next breeding.

Key words: Flue-cured tobacco, EMS induced mutation, Glandular trichomes density, Chemical components, Aroma substance, Mutant screening

Fig.1

The comparisons of leaf glandular trichomes density among mutant strains, Zhongyan100 and NC89 Different letters indicate significant difference at 5% level, the same below"

Fig.2

The comparison of cembrane content of leaf surface"

Fig.3

The comparison of alkanes content of leaf surface"

Fig.4

The comparison of sucrose esters content of leaf surface"

Table 1

Content of chemical components in mutants"

株系
Strain		 总氮(%)
Total nitrogen		 蛋白质(%)
Protein		 还原糖(%)
Reducing sugar		 总糖(%)
Total sugar		 烟碱(%)
Nicotine		 钾(%)
Potassium		 氯(%)
Chlorine		 总糖/烟碱
Total sugar/Nicotine		 还原糖/总糖
Reducing sugar/Total sugar		 钾/氯
K/Cl
EMS-1 2.18ab 11.41ab 15.87bc 21.13ab 2.55a 1.08b 0.24a 8.39ab 0.75b 4.48a
EMS-2 2.47a 12.22a 18.66ab 23.16a 2.33a 0.94b 0.22a 9.95a 0.81ab 4.51a
中烟100
Zhongyan100		 2.01b 10.45b 19.03a 21.93ab 3.04a 1.54a 0.32a 7.51b 0.87a 5.66a
NC89 2.11b 11.34ab 15.48c 19.66b 2.51a 0.99b 0.28a 7.86ab 0.78ab 3.55a

Fig.5

Analysis of netural aroma components in different strains"

Table 2

Sensory taste result of mutants"

株系
Srain		 香气质(9)
Aroma quality		 香气量(9)
Aroma amount		 浓度(9)
Concentrattion		 柔细度(9)
Tender degrees		 余味(9)
Aftertaste		 杂气(9)
Offensive odor		 刺激性(9)
Irritancy		 劲头(9)
Physiological strength		 总分
Total score
EMS-1 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 66.0
EMS-2 6.0 6.5 6.0 6.5 6.0 6.5 6.0 6.0 68.5
中烟100
Zhongyan100		 5.5 6.0 6.0 6.0 5.5 6.0 6.0 5.5 64.0
NC89 5.5 6.0 6.5 5.5 6.5 6.0 6.0 6.0 65.5
[1] 孙计平, 吴照辉, 李雪君 , 等. 烤烟品种中烟100与NC89及其F1主要特性研究. 河南农业科学, 2014,43(9):46-51,55.
doi: 10.3969/j.issn.1004-3268.2014.09.011
[2] 张杨, 裴军, 王方峰 , 等. 不同施肥水平对中烟100产质量的影响. 中国烟草科学, 2017(6):33-35.
doi: 10.3969/j.issn.1007-5119.2007.06.008
[3] 王正旭, 向德恩, 孟贵星 , 等. 施氮量和留叶数对中烟100产质量的影响. 中国烟草科学, 2017(S1):45-49.
doi: 10.3969/j.issn.1007-5119.2011.z1.010
[4] 李章海, 潘文杰, 朱晓兰 , 等. 不同烘烤方式烘烤过程中烟叶表面腺毛分泌物变化的研究. 中国烟草学报, 2017(6):81-85.
doi: 10.3969/j.issn.1004-5708.2011.06.016
[5] 王伟, 孔光辉, 李佛琳 , 等. 烤烟烟叶腺毛及其分泌物研究进展. 中国农学通报, 2017(2):251-254.
doi: 10.3969/j.issn.1000-6850.2007.02.059
[6] 平文丽, 金立锋, 张小全 , 等. 快中子辐射诱变K326突变体库的创建及初步分析. 烟草科技, 2018,51(1):21-27.
doi: 10.16135/j.issn1002-0861.2017.0356
[7] 朱晓宇, 姚珊珊, 赵宸楠 , 等. DIS过程中烤烟叶片腺毛形态结构及分泌物变化. 江西农业学报, 2017(5):9-11.
doi: 10.3969/j.issn.1001-8581.2010.05.003
[8] 崔清志, 刘晓虹, 陈惠明 . EMS诱变技术研究进展. 湖南农业科学, 2017(5):7-9.
doi: 10.3969/j.issn.1006-060X.2013.05.003
[9] 杨攀, 雷振生, 吴政卿 , 等. 航天诱变小麦新品种郑麦3596的选育. 作物杂志, 2017(5):163-167.
[10] 李小六, 张运峰, 张淑红 , 等. 原生质体EMS诱变选育杏鲍菇高产菌丝多糖菌株. 核农学报, 2017(8):1463-1468.
doi: 10.11869/j.issn.100-8551.2017.08.1463
[11] 佟星, 赵波, 金文林 , 等. 小豆EMS诱变M3代叶形突变体的筛选及农艺性状分析. 安徽农业科学, 2017(9):4472-4474.
doi: 10.3969/j.issn.0517-6611.2010.09.020
[12] 侯睿, 岳福良, 张小红 , 等. EMS诱变对花生油酸、蛋白质含量的影响. 西南农业学报, 2017(6):1245-1249.
doi: 10.16213/j.cnki.scjas.2016.06.002
[13] 姜颖, 孙宇峰, 潘冬梅 , 等. 工业大麻甲基磺酸乙酯(EMS)诱变体的筛选及RAPD分析. 作物杂志, 2017(6):50-54.
doi: 10.16035/j.issn.1001-7283.2017.06.009
[14] 高志勇, 谢恒星, 王志平 , 等. 植物突变体库的作用及构建研究进展. 作物杂志, 2017(6):16-19.
doi: 10.16035/j.issn.1001-7283.2016.06.003
[15] 刘威, 杨峰, 任旭东 , 等. EMS对种子萌发影响的进展. 分子植物育种, 2017,15(11):4585-4589.
[16] 韩锦峰, 张志勇, 刘华山 , 等. 烟草腺毛及其分泌物西柏三烯醇类物质的研究进展. 中国烟草学报, 2017(5):118-124.
doi: 10.3969/j.issn.1004-5708.2013.05.021
[17] 王瑞新 . 烟草化学. 北京:中国农业出版社, 2003: 1305-1312.
[18] 李艳华, 张洪映, 魏跃伟 , 等. 典型烤烟品种腺毛形态及分泌特性比较分析. 中国烟草学报, 2017,23(2):84-91.
doi: 10.16472/j.chinatobacco.2016.315
[19] 杜咏梅, 郭承芳, 张怀宝 , 等. 水溶性糖、烟碱、总氮含量与烤烟吃味品质的关系研究. 中国烟草科学, 2017(1):9-12.
doi: 10.3969/j.issn.1007-5119.2000.01.008
[20] 何承刚, 曾旭波 . 烤烟香气物质的影响因素及其代谢研究进展. 中国烟草科学, 2017(2):40-43.
doi: 10.3969/j.issn.1007-5119.2005.02.013
[21] 陈顺辉, 李文卿, 江荣风 , 等. 施氮量对烤烟产量和品质的影响. 中国烟草学报, 2017(S):36-40.
doi: 10.3321/j.issn:1004-5708.2003.z1.006
[22] 刘贯山 . 烟草突变体的筛选与鉴定. 中国烟草科学, 2017(1):102-103.
[23] 叶敬忠, 李华 . 关于转基因技术的综述与思考. 农业技术经济, 2017(1):11-21.
[24] 过伟民, 张艳玲, 蔡宪杰 , 等. 光质对烤烟品质及光合色素含量的影响. 烟草科技, 2017(9):65-70.
doi: 10.3969/j.issn.1002-0861.2011.09.015
[25] 史宏志, 官春云 . 烟草腺毛分泌物的化学成分及遗传. 作物研究, 1995,9(3):46-49.
[26] 付玉兰, 杨焕文, 邓建华 , 等. 津巴布韦引进与云南主栽烤烟品种腺毛密度及分泌物的差异. 烟草科技, 2017(9):82-86.
doi: 10.3969/j.issn.1002-0861.2012.09.018
[27] 薛晓明 . 烤烟腺毛的形态学观察和密度研究. 中国农学通报, 2017(4):98-103.
doi: 10.3969/j.issn.1000-6850.2012.04.018
[28] 杨铁钊, 李伟, 李钦奎 , 等. 烤烟叶面腺毛密度及其分泌物变化动态的相关分析. 中国烟草科学, 2017(1):43-46.
doi: 10.3969/j.issn.1007-5119.2005.01.014
[29] 王宝华, 吴帼英, 黄静勲 . 烟叶植物学特性的观察Ⅰ、烤烟烟叶腺毛的密度及其与烟叶品质和化学成分的关系. 中国烟草科学, 2017(2):1-6.
[30] 周昆, 周清明, 胡晓兰 . 烤烟香气物质研究进展. 中国烟草科学, 2008,29(2) : 58-61.
doi: 10.3969/j.issn.1007-5119.2008.02.014
[31] 薛晓明, 张晶, 于丽杰 . 烤烟腺毛分泌细胞的超微结构观察. 贵州农业科学, 2017(8):45-48.
doi: 10.3969/j.issn.1001-3601.2011.08.013
[32] 龚丝雨, 钟思荣, 张世川 , 等. 增施生物炭对烤烟生长及产量、质量的影响. 作物杂志, 2017(2):154-160.
doi: 10.16035/j.issn.1001-7283.2018.02.027
[33] 陆永恒 . 生态条件对烟叶品质影响的研究进展. 中国烟草科学. 2017(3):43-46.
[34] 高华军, 林北森 . 生物钾肥对烤烟产质量影响的研究进展. 中国烟草科学, 2017(3):73-76.
doi: 10.3969/j.issn.1007-5119.2009.03.016
[35] 刘燕, 孙焕良, 李辉 , 等. 烟草腺毛密度和分泌物与烟叶品质的关系. 作物研究, 2012,26(6):737-739.
doi: 10.3969/j.issn.1001-5280.2012.06.31
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