Crops ›› 2019, Vol. 35 ›› Issue (1): 68-74.doi: 10.16035/j.issn.1001-7283.2019.01.011

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Screening of High Aroma Mutants from Progenies of EMS Mutagenized Flue-Cured Tobacco

Xinling Yang1,Qian Yao1,Wenli Ping2,Yiqiong Ma1,Baolin Wang1,Guotao Jia1,Yongfeng Yang1,Hong Cui3   

  1. 1 Technology Centre, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450000, Henan, China
    2 Institute of Tobacco Research, HAAS/Key Laboratory for Green Preservation & Control of Tobacco Disease & Pests in Huanghuai Tobacco Growing Area, Xuchang 460001, Henan, China;
    3 College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2018-09-12 Revised:2018-11-21 Online:2019-02-15 Published:2019-02-01
  • Contact: Yongfeng Yang

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Abstract:

In order to screen the high-aroma and high-quality flue-cured tobacco offspring, Zhongyan 100 was used as the experimental material, and EMS mutagenesis technology was employed to treat the Zhongyan 100 seed and screen out two mutations with high density of leaf glandular trichomes. The body material was evaluated by field test for its glandular trichomes density, chemical composition of the leaf surface, chemical composition after baking, aroma substance content and sensory quality evaluation, and compared with the normal tobacco Zhongyan 100 offspring and NC89. The results showed that the density of glandular trichomes of EMS-2 mutagenized strains was 1.8 times and 1.2 times that of Zhongyan 100 and NC89, respectively. The chemical composition analysis of leaf surface showed that EMS-1 mutagenized strains the contents of cembranes and alkanes were the highest. The content of the sucrose esters in the EMS-2 mutant strain was the most abundant. The chemical composition analysis of the EMS-2 mutant strain showed that the chemical components of the EMS-2 mutant strain were more harmonious, and the aroma components were the highest. Compared with Zhongyan 100 and NC89, it increased by 34.5% and 29.0%. Combined with the sensory evaluation of mutagenized offspring, it was concluded that the comprehensive quality of mutagenic strains was better, and the quality of tobacco leaves could be improved. The comprehensive quality of EMS-2 was the best and could be used as selected strains proceed to the next breeding.

Key words: Flue-cured tobacco, EMS induced mutation, Glandular trichomes density, Chemical components, Aroma substance, Mutant screening

Fig.1

The comparisons of leaf glandular trichomes density among mutant strains, Zhongyan100 and NC89 Different letters indicate significant difference at 5% level, the same below"

Fig.2

The comparison of cembrane content of leaf surface"

Fig.3

The comparison of alkanes content of leaf surface"

Fig.4

The comparison of sucrose esters content of leaf surface"

Table 1

Content of chemical components in mutants"

株系
Strain		 总氮(%)
Total nitrogen		 蛋白质(%)
Protein		 还原糖(%)
Reducing sugar		 总糖(%)
Total sugar		 烟碱(%)
Nicotine		 钾(%)
Potassium		 氯(%)
Chlorine		 总糖/烟碱
Total sugar/Nicotine		 还原糖/总糖
Reducing sugar/Total sugar		 钾/氯
K/Cl
EMS-1 2.18ab 11.41ab 15.87bc 21.13ab 2.55a 1.08b 0.24a 8.39ab 0.75b 4.48a
EMS-2 2.47a 12.22a 18.66ab 23.16a 2.33a 0.94b 0.22a 9.95a 0.81ab 4.51a
中烟100
Zhongyan100		 2.01b 10.45b 19.03a 21.93ab 3.04a 1.54a 0.32a 7.51b 0.87a 5.66a
NC89 2.11b 11.34ab 15.48c 19.66b 2.51a 0.99b 0.28a 7.86ab 0.78ab 3.55a

Fig.5

Analysis of netural aroma components in different strains"

Table 2

Sensory taste result of mutants"

株系
Srain		 香气质(9)
Aroma quality		 香气量(9)
Aroma amount		 浓度(9)
Concentrattion		 柔细度(9)
Tender degrees		 余味(9)
Aftertaste		 杂气(9)
Offensive odor		 刺激性(9)
Irritancy		 劲头(9)
Physiological strength		 总分
Total score
EMS-1 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 66.0
EMS-2 6.0 6.5 6.0 6.5 6.0 6.5 6.0 6.0 68.5
中烟100
Zhongyan100		 5.5 6.0 6.0 6.0 5.5 6.0 6.0 5.5 64.0
NC89 5.5 6.0 6.5 5.5 6.5 6.0 6.0 6.0 65.5
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