Crops ›› 2019, Vol. 35 ›› Issue (5): 52-56.doi: 10.16035/j.issn.1001-7283.2019.05.009

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Effects of Artificial Aging on Genetic Integrity of Elymus sibiricus L.

Huang Fan,Li Jun,Liu Lei,Li Zhiyong,Li Hongyan,Shi Wengui,Xie Yongfeng   

  1. Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Huhhot 010010, Inner Mongolia, China
  • Received:2018-11-01 Revised:2019-06-20 Online:2019-10-15 Published:2019-11-07
  • Contact: Zhiyong Li

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Abstract:

The effects of artificial aging on seed vigor and genetic integrity of gliadin in four Elymus sibiricus L. accessions were studied. The results showed that the germination rate, germination potential, germinating index and vigor index of the Elymus sibiricus L. seeds decreased gradually with the aging time prolonging, the germination potential and vigor index of 4 materials were highly significantly different from CK with the decrease of germination rate among them. The genetic integrity of gliadin also changed obviously compared to CK. Two and three types of gliadin spectrum of varieties Elymus sibiricus L. cv. Chuancao No.1 and Elymus sibiricus L. cv. Nongmu were reduced to only 1 type while the types of gliadin spectrum of 2 wild accessions decreased most obviously than CK when the germination rate reduced to about 55%. The genetic diversity index analysis on gliadin spectrum of Elymus sibiricus L. also showed the allele number of 4 materials was significantly different from that of the control when the germination rate was less than 55%. Therefore, it is recommended that 60%~70% as the reference standard of germination rate for renewingElymus sibiricus L. by reproduction and the lowest critical value is 55%.

Key words: Elymus sibiricus L., Genetic integrity, Seed vigor, Gliadin, Artificial aging

Table 1

Testing materials"

编号
Number		 种质名称
Germplasm name		 种质类型
Germplasm type		 选育单位/原产地
Breeding unit/Origin
G1 农牧 育成品种 内蒙古农业大学
G2 川草1号 育成品种 四川省草原科学研究所
G3 05189 野生材料 新疆乌鲁木齐
G4 05191 野生材料 西藏拉萨

Fig.1

Effects of artificial aging on seed vigor of Elymus sibiricus L. Different small letters indicate significant difference (P<0.05)"

Fig.2

Effects of artificial aging on the gliadin spectrum types of Elymus sibiricus L."

Table 2

Effects of artificial aging on the gliadin spectrum types and frequency of Elymus sibiricus L."

编号
Number		 发芽率(%)
Germination rate		 带型数
Number of gliadin spectrum type		 各带型频率Frequency of gliadin spectrum type (%)
Ⅰ Ⅱ Ⅲ Ⅳ Ⅴ Ⅵ Ⅶ
G1-CK 95.3 3 85.15 10.21 4.64 - - - -
G1-1 83.2 2 71.37 28.63 - - - - -
G1-2 53.9 1 100.00 - - - - - -
G1-3 23.7 1 100.00 - - - - - -
G2-CK 95.9 2 83.95 16.05 - - - - -
G2-1 81.6 2 73.28 26.72 - - - - -
G2-2 49.6 1 100.00 - - - - - -
G2-3 25.7 1 100.00 - - - - - -
G3-CK 92.3 6 75.44 3.33 12.33 3.46 3.33 2.11 -
G3-1 84.7 5 87.22 4.89 2.25 2.67 2.97 0.00 -
G3-2 44.1 4 82.53 4.44 6.36 6.67 0.00 0.00 -
G3-3 28.6 4 88.95 2.55 1.36 7.14 0.00 0.00 -
G4-CK 98.1 7 78.46 5.26 2.26 2.22 4.95 3.33 3.52
G4-1 84.0 6 79.21 7.89 3.22 3.67 3.08 2.93 0.00
G4-2 50.8 4 83.58 5.44 4.31 6.67 0.00 0.00 0.00
G4-3 29.3 4 89.95 6.57 2.38 1.10 0.00 0.00 0.00

Table 3

Genetic analysis of gliadin spectrum types of Elymus sibiricus L."

编号Number 等位基因数Na 有效等位基因数Ne 多样性指数He 香农指数I
G1-CK 1.4344±0.0095 1.4110±0.0019 0.2147±0.0057 0.2966±0.0020
G1-1 1.3753±0.0389 1.4035±0.0051 0.2104±0.0059 0.2881±0.0066
G1-2 1.3616±0.0401* 1.4022±0.0072 0.2013±0.0064* 0.2846±0.0058
G1-3 1.3603±0.0352* 1.3917±0.0130 0.2001±0.0056* 0.2817±0.0080
G2-CK 1.4385±0.0056 1.4099±0.0047 0.2107±0.0054 0.2971±0.0034
G2-1 1.4299±0.0030 1.4080±0.0061 0.2090±0.0069 0.2909±0.0041
G2-2 1.4164±0.0064* 1.4056±0.0052 0.1945±0.0067* 0.2874±0.0064
G2-3 1.4095±0.0083* 1.3954±0.0118 0.1921±0.0054* 0.2847±0.0081
G3-CK 1.4416±0.0026 1.3438±0.0041 0.1987±0.0044 0.2824±0.0049
G3-1 1.4365±0.0047 1.3423±0.0021 0.1971±0.0049 0.2774±0.0026
G3-2 1.4178±0.0051* 1.3393±0.0017 0.1939±0.0054 0.2745±0.0034
G3-3 1.4117±0.0088* 1.3372±0.0039 0.1930±00.050 0.2693±0.0043
G4-CK 1.6596±0.0079 1.4415±0.0036 0.2397±0.0071 0.3703±0.0052
G4-1 1.6537±0.0078 1.4364±0.0025 0.2366±0.0058 0.3655±0.0062
G4-2 1.6371±0.0089* 1.4300±0.0102 0.2338±0.0061 0.3639±0.0073
G4-3 1.6296±0.0062* 1.4298±0.0109 0.2302±0.0081 0.3610±0.0080
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