Crops ›› 2019, Vol. 35 ›› Issue (6): 177-181.doi: 10.16035/j.issn.1001-7283.2019.06.028

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Pathogen Identification and Fungicide Screening of Stem Base Rot Disease on Pinellia pedatisecta

Han Xing1,2,He Yunzhuan1,Kang Zhanhai1,Wang Gaoyang1,Wang Bing1,Li Yaning1,Liu Daqun1,2   

  1. 1College of Plant Protection, Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, Hebei, China
    2Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2018-09-28 Revised:2019-08-27 Online:2019-12-15 Published:2019-12-11
  • Contact: Yaning Li

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Abstract:

Stem base rot is a serious disease with high incidence rate on the production of Pinellia pedatisecta in Anguo city, Hebei Province. Isolation, identification, pathogenicity and screening of its fungicides were carried out in our study. The pathogen was identified as Pythium aphanidermatum through measuring morphological characteristics and rDNA-ITS sequence analysis. Toxicity of five common chemical fungicides, two biological fungicides and crude antibiotic extracts of Streptomyces roseoflavus Men-myco-93-63 to Py. aphanidermatum was determined in laboratory. The results showed that 35% metalaxyl-M ES, crude antibiotic extracts of S. roseoflavus Men-myco-93-63, 40% flusilazole EC, and 99% hymexazol TC had certain inhibition with EC50 of 12.81, 41.74, 54.16, and 56.90μg/mL respectively. While the inhibitory effects of 1×10 11 spores per gram Bacillus subtilis WP, 1×10 6spores per gram Py. oligandrum WP, 3% metalaxyl·hymexazol AS and 50% dimethomorph WP were not obvious.

Key words: Pinellia pedatisecta, Stem base rot disease, rDNA-ITS, Pythium aphanidermatum, Toxicity measurement

Table 1

Concentration setting of each tested fungicides"

供试药剂Test fungicide 浓度梯度Concentration set (mg/L)
3%甲霜·恶霉灵AS 3% metalaxyl·hymexazol AS 25 50 100 200 500
50%烯酰吗啉WP 50% dimethomorph WP 10 20 50 200 500
40%氟硅唑EC 40% flusilazole EC 10 20 40 80 160
99%恶霉灵TC 99% hymexazol TC 25 50 100 200 400
35%精甲霜灵ES 35% metalaxyl-M ES 1.25 2.5 5 10 20
1×106孢子/g寡雄腐霉菌WP 1×106 spores per gram Py. oligandrum WP 2.5 5 10 20 50
1×1011芽孢/g枯草芽孢杆菌WP 1×1011 spores per gramBacillus subtilis WP 0.2 1 5 25 125
玫瑰黄链霉菌Men-myco-93-63抗生素粗提物 Crude antibiotic extracts of S. roseoflavus Men-myco-93-63 12.5 25 50 100 200

Fig.1

Phylogenetic tree of N4 based on rDNA-ITS sequences"

Table 2

Toxicity test of fungicides to Py. aphanidermatum in laboratory"

编号
No.		 供试药剂Test fungicide 毒力回归方程
Virulence regression equation		 R2 EC50(μg/mL)
1 35%精甲霜灵ES 35% metalaxyl-M ES Y=1.00X+3.89 0.974 12.81
2 玫瑰黄链霉菌Men-myco-93-63抗生素粗提物
Crude antibiotic extracts of S. roseoflavus Men-myco-93-63		 Y=1.55X+2.49 0.988 41.74
3 40%氟硅唑EC 40% flusilazole EC Y=1.70X+2.05 0.992 54.16
4 99%恶霉灵TC 99% hymexazol TC Y=1.59X+2.20 0.990 56.90
5 1×1011芽孢/g枯草芽孢杆菌WP 1×1011spores per gram B. subtilis WP Y=0.49X+3.76 0.929 359.80
6 1×106孢子/g寡雄腐霉菌WP 1×106 spores per gram Py. oligandrum WP Y=0.54X+3.59 0.976 414.50
7 3%甲霜·恶霉灵AS 3% metalaxyl·hymexazol AS Y=0.83X+2.56 0.981 864.00
8 50%烯酰吗啉WP 50% dimethomorph WP Y=1.13X+1.28 0.970 1 940.00
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