Crops ›› 2019, Vol. 35 ›› Issue (6): 14-19.doi: 10.16035/j.issn.1001-7283.2019.06.003

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Detection of Three Dwarfing Genes in the New Wheat Cultivars (Lines) Developed in South Huang-Huai Valley and Its Association with Agronomic Traits

Cao Tingjie1,Zhang Yu’e1,Hu Weiguo1,Yang Jian1,Zhao Hong1,Wang Xicheng1,Zhou Yanjie1,Zhao Qunyou2,Li Huiqun3   

  1. 1Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
    2Seed Management Department of Nanyang, Nanyang 473000, Henan, China
    3Seed Management Department of Puyang, Puyang 457000, Henan, China
  • Received:2019-05-28 Revised:2019-08-21 Online:2019-12-15 Published:2019-12-11

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Abstract:

In this study, Zhengmai 583 and 630 materials derived from variety comparison test and regional trials of Yellow-Huai wheat area and Henan Province at 2015-2016 were screened by specific molecular marker of wheat dwarf genes RhtB1-b, RhtD1-b and Rht8. The results showed that 549 wheat materials carried RhtB1-b gene; 592 wheat materials carried RhtD1-b gene; 513 wheat materials carried Rht8 gene; 422 materials carried all 3 dwarfing genes, and additional 169 lines carried 2 dwarfing genes which indicated that the 3 dwarf genes had been fixed in Henan wheat breeding progress. Moreover, Rht8 showed significantly correlation with reducing plant height, spike number per hectare and increasing 1000-kernel weight. Zhengmai 583 carried 3 dwarf genes, showed excellent fertility. The selection and utilization of dwarf genes are of certain value for the cultivation of wheat varieties with high yield.

Key words: Wheat, Plant height, Dwarfing gene, Agronomic traits

Table 1

Molecular markers for detection of RhtB1-b、RhtD1-b and Rht8"

检测基因名称
ID of detection gene		 正向引物序列(5′-3′)
Sequence of forward primer (5′-3′)		 反向引物序列(5′-3′)
Sequence of reverse primer (5′-3′)		 退火温度(℃)
Annealing temperature
RhtB1-b TCTCCTCCCTCCCCACCCAAC CATCCCCATGGCCATCTCGAGCTA 63
RhtD1-b CGCGCAATTATTGGCCAGAGATAG CCCCATGGCCATCTCGAGCTGCTA 63
Rht8 CTCCCTGTACGCCTAAGGC CTCGCGCTACTAGCCATTG 55

Fig.1

Amplification results of RhtB1-b (a) and RhtD1-b (b) primers in some tested cultivars (lines) M. Marker; 1. Norin 10; 2. Zhengmai 583; 3. Shengmai 197; 4. Xinliang 3; 5. Guangtai 68; 6. Xumai 0054; 7. Quanmai 890; 8. Xinong 501. The same below"

Fig.2

PCR amplification result of 4 varieties of Rht8 in test cultivars (lines) 9. Fengdecunmai 12; 10. Longke 1221. a. 192bp; b. 202bp; c. 165bp; d. 174bp"

Fig.3

Distribution frequency of RhtB1-b, RhtD1-b and Rht8 haplotypes in 631 wheat cultivars (lines)"

Table 2

Descriptive statistics of agronomic traits in 631 wheat cultivars (lines)"

性状Trait 最小值
Minimum		 最大值
Maximum		 平均值
Mean		 标准差
Standard deviation		 变异系数(%)
Coefficient of variation
株高Plant height (cm) 59.30 91.00 76.99 4.51 0.06
每公顷穗数Spike number per hectare (×106) 3.80 6.63 5.57 0.45 0.08
千粒重1000-kernel weight(g) 37.00 53.40 45.00 2.71 0.06
穗粒数Kernel number per spike 29.10 43.80 34.80 2.19 0.06

Fig.4

Scatter plot matrix of 4 agronomic traits Histogram represents the frequency distribution of 4 traits; Lower left quarter plot elements display the pairwise two-dimensional scatter plot (the straight fitting line); Upper right corner display the pairwise correlation coefficient ("*" P<0.05)"

Table 3

Effects of Rht8 on 4 agronomic traits"

性状
Trait		 位点
Locus		 样本容量
Sample size		 平均数
Mean		 标准差
Standard error		 最小值
Minimum		 最大值
Maximum		 F 显著性
Significance
株高Plant height (cm) Rht8(+) 513 76.70 4.44 59.30 89.00 11.53 0.001**
Rht8(-) 117 78.26 4.61 63.90 91.00
每公顷穗数Spike number per hectare (×106) Rht8(+) 513 5.54 0.36 3.80 6.63 10.12 0.002**
Rht8(-) 117 5.66 0.48 4.40 6.63
千粒重1000-kernel weight (g) Rht8(+) 513 45.10 2.66 37.80 53.40 4.08 0.044*
Rht8(-) 117 44.54 2.90 37.00 50.90
穗粒数Kernel number per spike Rht8(+) 513 34.93 2.58 29.10 43.80 15.19 0.000**
Rht8(-) 117 34.06 2.41 29.40 40.61
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