Crops ›› 2021, Vol. 37 ›› Issue (3): 210-216.doi: 10.16035/j.issn.1001-7283.2021.03.032

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Effects of Selenium on Seed Germination, Physiological Characteristics and Nutritional Quality of Kidney Bean

Shi Yahan(), Du Tianqing(), Zhai Hongmei, Yang Shutian, Gong Rui, Li Yuhang, Lu Boyu, Cui Fuzhu, Gao Zhiqiang   

  1. College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China
  • Received:2020-05-27 Revised:2021-02-22 Online:2021-06-15 Published:2021-06-22
  • Contact: Du Tianqing E-mail:363274276@qq.com;tgdtq1968@sina.com

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Abstract:

In order to explore the effects of selenium on the seed germination, physiological characteristics and nutritional quality of kidney bean seeds, the "British Red" kidney bean variety was used as material for germination test and field trial. The germination test set seven selenium soaking concentration treatments, 0 (J0), 7.5 (J1), 15.0 (J2), 22.5 (J3), 30.0 (J4), 37.5 (J5) and 45.0mg/L (J6). In the field experiment, four selenium spraying doses were set: 0 (P0), 10 (P1), 20 (P2) and 30g/hm2 (P3). The results showed that 30.0mg/L selenium soaking concentration significantly promoted the germination of kidney bean seeds. Compared with the control (no selenium),under the condition of spraying with 30g/hm2 selenium, the POD activity, GSH content, and chlorophyll content of kidney bean leaves increased by 35.29%, 32.34% and 7.92%, respectively, and the MDA content decreased by 20.32%; the soluble sugar, sucrose, crude fat, crude protein, crude starch, and selenium content of grains increased by 23.65%, 37.23%, 75.00%, 62.14%, 32.21%, 24.11%, respectively.

Key words: Selenium, Germination, Kidney bean, Physiological characteristics, Nutritional quality

Table 1

Effects of seed soaking with selenium on seed germination of kidney bean"

处理
Treatment		 发芽势
Germination vigor (%)		 发芽率
Germination percentage (%)		 发芽指数
Germination index		 活力指数
Vitality index
J0 78.5de 79.7e 19.4b 1.27d
J1 80.2cd 82.2d 19.7b 1.32d
J2 81.3bc 84.7c 29.8a 1.61c
J3 83.2b 86.5b 30.9a 1.95b
J4 88.2a 93.5a 31.2a 2.43a
J5 80.2cd 83.7cd 17.2b 1.63c
J6 76.7e 79.5e 16.9b 1.30d

Fig.1

Effects of foliar selenium spraying on physiological indexes of kidney bean Different lowercase letters indicate significant difference at the level of P < 0.05, the same below"

Fig.2

The effects of foliar selenium spray on selenium content in kidney bean grains"

Table 2

The effects of foliar selenium spraying on the nutritional quality of kidney bean"

处理
Treatment		 可溶性糖含量
Soluble sugar content (mg/g)		 蔗糖含量
Sucrose content (mg/g)		 粗脂肪含量
Crude fat content (%)		 粗蛋白含量
Crude protein content (%)		 粗淀粉含量
Crude starch content (%)
P0 77.8±3.52b 41.9±3.92b 0.8±0.09c 14.0±0.81c 26.7±0.94d
P1 87.0±4.26ab 42.3±4.00b 1.1±0.07b 17.3±1.25b 28.3±0.47c
P2 92.5±2.17a 56.3±5.57a 1.3±0.02a 20.3±0.47a 32.3±1.70b
P3 96.2±4.24a 57.5±5.91a 1.4±0.02a 22.7±1.25a 35.3±1.25a

Table 3

Correlation analysis of selenium content and kidney bean physiological characteristics and nutritional quality"

指标
Index		 硒含量
Se
content		 POD活性
POD
activity		 MDA含量
MDA
content		 GSH含量
GSH
content		 叶绿素含量
Chlorophyll content		 粗脂肪含量
Crude fat content		 粗蛋白含量
Crude protein content		 粗淀粉含量
Crude starch content		 可溶性糖含量
Soluble sugar content		 蔗糖含量
Sucrose content
硒含量
Se content		 1.0000
POD活性
POD activity		 0.968** 1.0000
MDA含量
MDA content		 -0.970** -0.947** 1.0000
GSH含量
GSH content		 0.975** 0.943** -0.971** 1
叶绿素含量
Chlorophyll content		 0.638*0 0.646* -0.671** 0.719** 1
粗脂肪含量
Crude fat content		 0.952** 0.952** -0.938** 0.940** 0.613* 1
粗蛋白含量
Crude protein content		 0.944** 0.946** -0.936** 0.957** 0.693** 0.883** 1
粗淀粉含量
Crude starch content		 0.908** 0.861** -0.859** 0.945** 0.637* 0.886** 0.882** 1
可溶性糖含量
Soluble sugar content		 0.872** 0.890** -0.849** 0.872** 0.594* 0.815** 0.952** 0.805** 1
蔗糖含量
Sucrose content		 0.784** 0.701** -0.773** 0.751** 0.453 0.695** 0.655* 0.652* 0.472 1
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