Crops ›› 2021, Vol. 37 ›› Issue (4): 206-211.doi: 10.16035/j.issn.1001-7283.2021.04.032

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Effects of Hydrogen-Rich Water on Barley Seed Germination and Barley Seedling Biomass Distribution under Drought Stress

Song Ruijiao(), Feng Caijun, Qi Juncang()   

  1. Agricultural College of Shihezi University/The Key Laboratory of Oasis Eco-Agriculture, Xinjiang Production and Construction Corps, Shihezi 832003, Xinjiang, China
  • Received:2020-08-14 Revised:2020-12-24 Online:2021-08-15 Published:2021-08-13
  • Contact: Qi Juncang E-mail:435991524@qq.com;shzqjc@qq.com

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Abstract:

To investigate the effects of hydrogen-rich water (HRW) on drought resistance in germination period and seedling biomass distribution under drought stress, barley variety Xinpi 6 was used as material, which cultured in 20% polyethylene glycol-6000 (PEG). The 16 indexes were compared in different concentrations of HRW, such as germination rate and seedling root-shoot ratio. The results showed that the suitable concentration of HRW treatment could significantly increase the germination rate, dry matter transfer amount, transfer rate, and conversion efficiency; reduced respiratory consumption of dry matter and root-shoot ratio; increased the roots and buds’ dry weight; promoted the accumulation of soluble sugar, soluble protein, and chlorophyll in barley seedlings. It could be concluded that suitable concentration of HRW could increase the germination rate of barley seeds under drought stress by regulating the transport of dry matter, and reduce the adverse effects of drought stress on the proportion of root and bud biomass of seedlings by regulating the contents of soluble sugar, soluble protein, and chlorophyll.

Key words: Hydrogen-rich water, Drought stress, Barley, Germination

Fig.1

Germination rate of barley seeds soaked in different concentrations HRW under drought stress Different lowercase letters indicate significant differences between treatments (P < 0.05), different capital letters indicate extremely significant differences between treatments (P < 0.01), the same below"

Table 1

The effects of seed soaking in different concentrations HRW on barley seed dry matter transport under drought stress"

富氢水浓度
HRW
concentration (%)		 干物质转移量
Dry matter transfer amount (mg)		 干物质转移率
Dry matter transfer
rate (%)		 呼吸消耗干物质量
Respiratory consumption
of dry matter (mg)		 干物质转化效率
Dry matter conversion
efficiency (%)
0(CK) 426.39±20.15bA 67.72±3.20bA 313.79±22.98aA 26.59±1.98bBC
25 454.13±8.20abA 72.12±1.31abA 302.89±12.83bA 33.36±1.64aB
50 465.49±19.97abA 73.93±3.17abA 303.79±20.49bA 34.87±1.58aA
75 474.26±2.20aA 75.32±0.35aA 310.89±8.68bA 34.45±1.61aB
100 482.59±10.51aA 76.64±1.67aA 362.93±15.91aA 24.87±1.73aC

Table 2

The effects of seed soaking in different concentrations HRW on root and bud biomass distribution of barley seedlings under drought stress"

富氢水浓度
HRW concentration (%)		 幼苗干重
Seedling dry weight (mg)		 根干重
Root dry weight (mg)		 芽干重
Bud dry weight (mg)		 根冠比
Root-shoot ratio
0(CK) 112.60±3.10bB 72.10±2.48cC 40.50±2.35cB 1.79±0.14abAB
25 151.23±4.72aA 82.70±4.69bcBC 68.53±0.68aA 1.21±0.07cB
50 161.70±1.76aA 91.97±2.40bAB 69.73±2.11aA 1.32±0.07bcAB
75 163.37±7.15aA 105.87±6.95aAB 57.50±1.81bA 1.85±0.13abAB
100 119.67±5.82bB 79.80±0.81bcBC 39.87±6.54cB 2.10±0.31aA

Fig.2

The effects of seed soaking in different concentrations HRW on barley seedling soluble sugar contents of root and bud under drought stress"

Fig.3

The effects of seed soaking in different concentrations HRW on barley seedling soluble protein contents of root and bud under drought stress"

Table 3

The effects of seed soaking in different concentrations HRW on barley seedling chlorophyll a, chlorophyll b and total chlorophyll contents under drought stress mg/g"

富氢水浓度
HRW concentration (%)		 叶绿素a
Chlorophyll a		 叶绿素b
Chlorophyll b		 叶绿素总含量
Total chlorophyll content
0(CK) 780.07±23.21dC 249.68±21.52abA 1029.76±33.64cD
25 1173.08±63.89aA 259.51±15.37aA 1432.59±78.98aA
50 1034.44±34.40bB 218.03±7.17bA 1252.48±41.51bB
75 968.96±8.45bB 214.88±2.79bA 1183.84±9.79bBC
100 850.46±33.16cC 219.22±34.81bA 1069.69±67.54cCD
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