Crops ›› 2022, Vol. 38 ›› Issue (3): 104-108.doi: 10.16035/j.issn.1001-7283.2022.03.015

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Bioinformatics and Expression Analysis of HaLACS7 Gene in Sunflower

Zhou Fei1,2()   

  1. 1Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086, Heilongjiang, China
    2Industrial Crops Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
  • Received:2021-04-12 Revised:2021-07-01 Online:2022-06-15 Published:2022-06-20

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Abstract:

LACS encodes for long-chain acyl-CoA synthetase, a protein that converts free fatty acids into long-chain acyl-CoA, an essential lipid metabolite. The bioinformatics analysis of the HaLACS7 encoded protein in sunflower was carried out in this study. The protein encoded by HaLACS7 was expected to be an acidic and hydrophilic protein. The protein encoded by HaLACS7 had an irregular coil secondary structure, and it’s subcellular location was anticipated to be in the peroxisome. With 84% sequence similarity, HaLACS7 was shown to be closely related to Lactuca sativa L. and Cynara cardunculus var. Scolymus L. LACS6, and clustered in a broad branch with LACS6 and LACS7 of Arabidopsis thaliana L. The expression level of HaLACS7 in sunflower tissues was ligulate flowers > root > seed > tubular flower > leaf > stem. The expression level of HaLACS7 was higher at the late development stage of high and low oleic acid sunflower seeds, but the expression trend was significantly different at different seed development stages of the two materials. Therefore, it was suggested that the expression level of HaLACS7 may affect the synthesis of oleic acid in sunflower seeds.

Key words: Sunflower, Oleic acid, HaLACS7, Bioinformatics, Expression anaylsis

Table 1

Amino acid composition of protein encoded by HaLACS7 in sunflower"

种类Category 数量Quantity 质量分数Mass fraction (%) 种类Category 数量Quantity 质量分数Mass fraction (%)
丙氨酸Ala (A) 18 5.7 甲硫氨酸Met (M) 7 2.2
精氨酸Arg (R) 14 4.4 苯丙氨酸Phe (F) 18 5.7
天冬酰胺Asn (N) 17 5.4 脯氨酸Pro (P) 18 5.7
天冬氨酸Asp (D) 17 5.4 丝氨酸Ser (S) 21 6.7
半胱氨酸Cys (C) 10 3.2 苏氨酸Thr (T) 12 3.8
谷氨酰胺Gln (Q) 12 3.8 色氨酸Trp (W) 6 1.9
谷氨酸Glu (E) 20 6.3 酪氨酸Tyr (Y) 12 3.8
甘氨酸Gly (G) 19 6.0 缬氨酸Val (V) 27 8.6
组氨酸His (H) 5 1.6 吡咯赖氨酸Pyl (O) 0 0.0
异亮氨酸Ile (I) 24 7.6 赖氨酸Lys (K) 19 6.0
亮氨酸Leu (L) 19 6.0 硒代半胱氨酸Sec (U) 0 0.0

Fig.1

Secondary structure prediction of protein encoded by HaLACS7 in sunflower"

Fig.2

Hydrophilic/hydrophobic analysis of protein encoded by HaLACS7 in sunflower"

Fig.3

Phylogenetic tree analysis of HaLACS7 gene in sunflower"

Fig.4

The expression patterns of HaLACS7 gene at different development stages and different parts"

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