Crops ›› 2022, Vol. 38 ›› Issue (5): 146-152.doi: 10.16035/j.issn.1001-7283.2022.05.021

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Analysis of Physiological and Biochemical Mechanism and Growth and Development Characteristics of Saline and Alkali Resistance in Sunflower

Jia Xiuping1(), Mao Xuhui1, Liang Gensheng1, Liu Runping2, Liu Feng2, Wang Xingzhen1   

  1. 1Institute of Crop, Gansu Academy of Agriculture Sciences, Lanzhou 730070, Gansu, China
    2Institute of Agricultural Economics and Information, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
  • Received:2021-05-18 Revised:2021-08-16 Online:2022-10-15 Published:2022-10-19

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Abstract:

A total of 135 strains of the F2 population, produced by crossing of Y05-222A and Y06-136R, were used as materials to assess the levels of proline (Pro), soluble protein, malondialdehyde (MDA) as well as the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), and variance, correlation, and path analyses were performed. The F2 populationʼs skewness and kurtosis were also examined. The outcomes demonstrated that the frequencies followed a normal distribution and the six physiological indexes of the F2 population were P > 0.05. It simultaneously demonstrated the separation of the bidirectional superaffinity. The six physiological indexes had highly correlations with salinity and alkali resistance coefficients, according to correlation and path analysis. The direction of the total indirect path coefficient and correlation was identical. The direct path coefficient of POD activity was 0.5003, which indicated it directly affected saline and alkali resistance. The direct path coefficients of CAT activity and Pro content were -0.1317 and -0.0384, respectively, which indicated it indirectly affected saline and alkali resistance, and SOD, MDA and soluble protein directly or indirectly affected salinity resistance. The Anti-saline effects of POD, SOD, Pro, CAT, soluble protein, MDA showed POD > CAT > Pro > soluble protein > MDA > SOD. Number of leaves, plant height, stem diameter and disk diameter had significant positive correlation with the salinity and alkali resistance coefficient. And the correlation showed number of leaves > disk diameter > plant height > stem diameter. The results provide theoretical basis for salinity salt and alkali resistance of oil sunflower.

Key words: Oil sunflower, F2 populations, Correlation analysis, Path analysis

Table 1

Performance and analysis of specificity of physiological indicators in the F2 population and its parents"

指标
Index		 亲本Parents F2群体F2 population
P1 P2 F检测值
F-value		 均值
Mean		 变异范围
Range of
variation		 偏度数
Skewness		 偏度系数P值
Skewness P
value		 峰度
系数
Kurtosis		 峰度系数P值
Kurtosis
P value
SOD活性
SOD activity (U/mg)		 660.47±10.96 346.58±10.84 868.31** 552.61 100.86~897.25 -0.03 0.90 -0.39 0.35
POD活性
POD activity (U/g)		 24.63±1.15 12.27±0.48 295.21** 45.60 4.67~91.67 0.15 0.49 -0.30 0.47
CAT活性
CAT activity (U/g)		 12.22±0.98 2.58±0.19 93.69** 32.86 2.39~66.00 0.21 0.30 0.00 0.99
可溶性蛋白含量
Soluble protein content (mg/g)		 191.90±6.57 104.63±10.47 149.62** 24.10 9.79~41.67 0.11 0.59 -0.61 0.14
Pro含量
Pro content (µg/g)		 146.15±6.61 95.67±4.92 112.63** 219.49 120.79~354.65 0.25 0.21 -0.38 0.36
MDA含量
MDA content (µmol/g)		 1.14±0.03 1.51±0.44 157.96** 57.97 16.50~94.49 0.26 0.21 0.02 0.99
抗盐碱系数
Salt-alkali resistance coefficient		 0.75±0.07 0.54±0.01 27.80** 0.70 0.22~0.90

Fig.1

Frequency distribution of related indicators for F2 population"

Table 2

Correlation analysis salt-alkali resistance coefficient and related indicators in the F2 population"

指标
Index		 SOD活性
SOD
activity		 POD活性
POD
activity		 CAT活性
CAT
activity		 可溶性蛋白含量
Soluble
protein content		 Pro含量
Pro
content		 MDA含量
MDA
content		 抗盐碱性系数
Salt-alkali resistance
coefficient
SOD活性SOD activity 1.0000
POD活性POD activity 0.6577** 1.0000
CAT活性CAT activity 0.6455** 0.6681** 1.0000
可溶性蛋白含量Soluble protein content -0.7061** -0.5194** -0.5262** 1.0000
Pro含量Pro content 0.7935** 0.6493** 0.8013** -0.6383** 1.0000
MDA含量MDA content -0.5347** -0.5967** -0.5123** 0.4699** -0.5563** 1.0000
抗盐碱系数
Salt-alkali resistance coefficient		 0.3904** 0.5256** 0.2987** -0.2675** 0.3298** -0.3725** 1.0000

Table 3

Path analysis of salt-alkali resistance coefficient and its indexes of F2 population"

指标
Index		 直接通径系数
DPC		 间接通径系数IPC 总间接通径系数
TIPC
X1→Y X2→Y X3→Y X4→Y X5→Y X6→Y
X1 0.1676 0.3291 -0.085 -0.0464 -0.0305 0.0556 0.2228
X2 0.5003 0.1102 -0.088 -0.0341 -0.0249 0.0621 0.0253
X3 -0.1317 0.1081 0.3343 -0.0346 -0.0308 0.0533 0.4303
X4 0.0657 -0.1183 -0.2599 0.0693 0.0245 -0.0489 -0.3333
X5 -0.0384 0.1329 0.3249 -0.1055 -0.0420 0.0579 0.3682
X6 -0.1040 -0.0896 -0.2985 0.0674 0.0309 0.0214 -0.2684

Table 4

Analysis of morphological characteristics in parents and F2 population"

性状
Trait		 亲本Parents F2群体F2 population
P1 P2 F检测值F detection value 均值Mean 变异范围Range of variation
株高Plant height (cm) 148.30 96.53 68.62** 110.98 36.42~166.18
茎粗Stem diameter (cm) 1.70 1.38 74.93** 1.42 0.54~2.25
叶片数Number of leaves 15.33 12.00 16.20* 8.96 5.00~12.00
盘径Disk diameter (cm) 12.97 10.07 30.66** 8.42 3.20~12.40

Table 5

Correlation analysis of salt-alkali resistance coefficient and morphological characteristics in F2 population"

性状
Trait		 株高
Plant height		 茎粗
Stem diameter		 叶片数
Number of leaves		 盘径
Disk diameter		 抗盐碱系数
Salt-alkali resistance coefficient
株高Plant height 1.0000
茎粗Stem diameter 0.8361** 1.0000
叶片数Number of leaves 0.9150** 0.7902** 1.0000
盘径Disk diameter 0.9378** 0.8180** 0.9238** 1.0000
抗盐碱系数Salt and alkali resistance coefficient 0.8501** 0.8440** 0.8786** 0.8759** 1.0000
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