Crops ›› 2020, Vol. 36 ›› Issue (3): 47-52.doi: 10.16035/j.issn.1001-7283.2020.03.008

Previous Articles     Next Articles

Drought-Resistance Identification and Evaluation of 152 Sunflower Recombinant Inbred Lines (RILs) at Seedling Stage

Zhou Zichao, Hou Jianhua(), Zhen Zilong, Shi Huimin   

  1. College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010010, Inner Mongolia, China
  • Received:2020-01-17 Revised:2020-04-08 Online:2020-06-15 Published:2020-06-10
  • Contact: Jianhua Hou E-mail:houjh68@163.com

RichHTML

8

PDF (PC)

274

Abstract:

In this experiment, 152 oil sunflower recombination inbred lines were used as test materials in greenhouse pot experiment, to study the effects of drought stress on their seedling phenotype, physiological and biochemical characteristics. The drought-resistance coefficient, comprehensive drought resistance (D-value), correlation analysis, linear regression analysis and cluster analysis were used for the calculation and analysis of data to select the simple and effective identification indexes and drought resistance germplasms of sunflower at seedling stage. The results showed that the drought-resistance coefficient from high to low is in order of root-shoot ratio, root surface area, root length, root volume, electrical conductivity, SPAD value, plant height, relative water content of leaves and leaf area. Linear regression analysis and correlation analysis of drought resistance D-value showed that the nine indicators were significantly correlated with drought-resistance coefficients at seedling stage out of which the root length and root surface area are more significantly related to drought-resistance coefficients. D-value clustering grouped the tested inbred line materials into 4 categories, including 2 strong drought-tolerant types, 2 drought tolerant types, 83 weak drought-tolerant types and 65 non-drought-tolerant types. Among them, the D-values of the comprehensive drought-resistant ability of strain 149 and strain 10 were significantly higher than that of other strains.

Key words: Sunflower, Seedling stage, Recombinant inbred line, Drought resistance

Table 1

Soil water content and watering amount"

胁迫天数
Days of
stressing (d)		 土壤含水量
Soil moisture content (%)		 浇水量
Watering amount (mL)
对照
Control		 干旱胁迫
Drought stress		 对照
Control		 干旱胁迫
Drought stress
3 18.5 8.5 120 60
6 18.0 7.5 160 120
9 17.0 8.0 200 160
12 16.5 7.8 320 200
15 19.0 8.8 400 320

Table 2

Descriptive statistics of drought-resistance indicators at seedling stage"

指标
Indicator		 均值Mean 变异幅度Variability 变异系数
Coefficient of variation (%)		 广义遗传力
Generalized heritability (%)		 抗旱系数
Drought-resistance coefficient (%)
对照
Control		 干旱胁迫
Drought stress		 对照
Control		 干旱胁迫
Drought stress		 对照
Control		 干旱胁迫
Drought stress
电导率Electrical conductivity (μS/cm) 0.66 0.81 0.69 0.70 20.68 14.34 90.93 122.7
根表面积Root surface area (cm2) 3.27 5.00 7.84 10.47 36.66 32.05 61.73 152.9
根冠比Root-shoot ratio 0.09 0.24 0.33 0.61 59.60 42.58 15.52 266.7
根长Root length (cm) 21.12 27.66 53.84 56.97 35.86 39.51 46.09 131.0
根体积Root volume (cm3) 0.12 0.15 0.75 0.38 61.66 38.81 80.00 125.0
SPAD值SPAD value 33.34 30.25 32.07 27.10 15.90 12.32 79.61 90.7
叶面积Leaf area (cm2) 13.15 4.39 6.87 7.85 40.81 30.37 53.18 33.4
叶片相对含水量
Relative water content of leaves (%)		 0.87 0.60 0.30 0.55 7.70 17.01 30.77 68.9
株高Plant height (cm) 16.95 14.32 23.17 31.67 18.60 26.43 83.73 84.3

Table 3

Correlation analysis of drought-resistance coefficients of all indicators at seedling stage"

指标
Indicator		 电导率
Electrical
conductivity		 根表面积
Root surface
area		 根冠比
Root-shoot
ratio		 根长
Root
length		 根体积
Root
volume		 SPAD值
SPAD
value		 叶面积
Leaf
area		 叶片相对含水量
Relative water
content of leaves
根表面积Root surface area -0.258**
根冠比Root-shoot ratio -0.180* -0.198*
根长Root length -0.197* -0.185* -0.235**
根体积Root volume -0.186* -0.194* -0.120 0.239**
SPAD值SPAD value -0.245** -0.323** -0.272** 0.268** -0.072
叶面积Leaf area -0.180* -0.178* -0.230** 0.008 -0.419** -0.295**
叶片相对含水量Relative water content of leaves -0.237** -0.240** -0.323** 0.291** -0.376** -0.343** 0.244**
株高Plant height -0.038 -0.249** -0.159* 0.258** -0.320** -0.283** 0.296** -0.048

Table 4

The linear regression models with drought-resistance coefficient as dependent variable and the comprehensive drought-resistance subordinate function value as predictive variable"

回归变量Regression variable 回归模型Regression model R2
电导率Electrical conductivity y=0.3771x-0.0220 0.9993
根表面积Root surface area y=0.2708x-0.2707 0.9995
根冠比Root-shoot ratio y=0.0655x-0.0037 0.9995
根长Root length y=0.3795x-0.2501 0.9996
根体积Root volume y=0.1800x-0.0176 0.9989
SPAD值SPAD value y=0.9363x-0.2073 0.9991
叶面积Leaf area y=0.8528x-0.0510 0.9991
叶片相对含水量
Relative water content of leaves		 y=1.2755x-0.5599 0.9992
株高Plant height y=0.4151x-0.2117 0.9987

Table 5

Drought-resistance comprehensive evaluation of 152 sunflower materials"

样品编号
Sample number		 综合抗旱能力D值
D value of drought-resistance
comprehensive evaluation		 D值排序
D-value
order
10 0.48 1
149 0.44 2
75、138 0.39 3
120 0.35 4
126、95、110、123 0.33 5
16、77、97、109 0.32 6
6、100、143 0.31 7
1、4、5、59、116、129、151 0.30 8
48、72、85、92、96、107、146 0.29 9
7、8、17、23、38、46、49、68、73、76、102、104、119、135 0.28 10
3、9、18、24、29、37、44、51、54、56、64、69、82、114、130、133、136、145 0.27 11
2、15、22、25、27、45、61、67、106、111、124、131 0.26 12
33、50、62、70、99、103、105、113、117、122、128、139、140 0.25 13
39、42、66、71、74、84、86、94、112、115、132、150 0.24 14
19、28、31、35、47、52、53、55、58、78、79、89、93、101、108、125、127、147 0.23 15
11、12、32、137、141、142、144 0.22 16
34、40、57、63、65、87、90、91、98、118、121、134 0.21 17
14、21、30、36、43、80、88、152 0.20 18
13、26、41、60、83 0.19 19
81、148 0.18 20
20 0.17 21

Fig.1

Cluster analysis 152 sunflower inbred lines based on D-value of drought-resistance comprehensive evaluation"

Table 6

Correlation analysis of drought-resistance coefficients and D-value of drought-resistance comprehensive evaluation of 9 indicators"

株高
Plant height		 叶片相对含水量
Relative water content of leaves		 叶面积
Leaf area		 根冠比
Root-shoot ratio		 电导率
Electrical conductivity		 根长
Root length		 根表面积
Root surface area		 根体积
Root volume		 SPAD值
SPAD value
0.256** 0.374** 0.462** 0.247** 0.183* 0.578** 0.403** 0.429** 0.169*
[1] 管晓丹, 石瑞, 孔祥宁 , 等. 全球变化背景下半干旱区陆气机制研究综述. 地球科学进展, 2018,33(10):995-1004.
[2] 张振宇, 刘丽娟, 刘小玉 . 干旱区膜下滴灌向日葵农田蒸散发特征. 中国农业生态学报(中英文), 2019,27(8):1195-1204.
[3] Jordaan E, van der Waals J E, McLaren N W . Effect of irrigation on charcoal rot severity,yield loss and colonization of soybean and sunflower. Crop Protection, 2019,122:63-69.
doi: 10.1016/j.cropro.2019.04.026
[4] Miner G L, Bauerle W L . Seasonal responses of photosynthetic parameters in maize and sunflower and their relationship with leaf functional traits. Plant, Cell & Environment, 2019,42(5):1561-1574.
[5] 王尊欣, 张树珍 . 作物抗旱性及抗旱育种研究进展. 作物杂志, 2014(2):26-31.
[6] 张海燕, 解备涛, 汪宝卿 , 等. 不同甘薯品种抗旱性评价及耐旱指标筛选. 作物学报, 2019,45(3):419-430.
[7] 杨如萍, 包振贤, 陈光荣 , 等. 大豆抗旱性研究进展. 作物杂志, 2012(5):8-12.
[8] 陈娇, 谢小玉, 张小短 , 等. 甘蓝型油菜苗期抗旱性鉴定及综合抗旱指标筛选. 中国油料作物学报, 2019,41(5):713-722.
[9] 张晓蒙, 菅志亮, 李素萍 . 向日葵种植存在问题及对策. 农业与技术, 2017,37(10):92.
[10] 柳延涛, 陈寅初, 李万云 , 等. 向日葵产业技术体系需求调研报告. 新疆农垦科技, 2012,35(10):3-6.
[11] 张立华 . 内蒙古向日葵生产的现状及发展对策. 呼和浩特:内蒙古农业大学, 2006.
[12] 钟娜 . DDS-11A型电导仪的使用维修. 企业标准化, 2004(12):47.
[13] 兰巨生 . 农作物综合抗旱性评价方法的研究. 西北农业学报, 1998,7(3):85-87.
[14] 陈立松, 刘星辉 . 作物抗旱鉴定指标的种类及其综合评价. 福建农业大学学报, 1997,26(1):48-55.
[15] Lan J S, Si B C . Soil trend effect on genetic correlation of crop properties. North China Agricultural Bulletin, 1994(S2):8-11.
[16] 龚明 . 作物抗旱性鉴定方法与指标及其综合评价. 云南农业大学学报, 1989,4(1):73-81.
[17] 马宇 . 向日葵苗期抗旱性评价及全基因组关联分析. 呼和浩特:内蒙古农业大学, 2018.
[18] 张鸿, 李其勇, 朱从桦 , 等. 作物抗旱性鉴定的主要评价方法. 四川农业科技, 2017(6):7-9.
[19] 孙彩霞, 沈秀瑛 . 玉米根系生态型及生理活性与抗旱性关系的研究. 华北农学报, 2002,17(3):20-24.
doi: 10.3321/j.issn:1000-7091.2002.03.004
[20] 孙彩霞, 沈秀瑛 . 作物抗旱性鉴定指标及数量分析方法的研究进展. 中国农学通报, 2002,18(1):49-51.
[21] 王贺正 . 水稻抗旱性研究及其鉴定指标的筛选. 雅安:四川农业大学, 2007.
[22] 陈志辉, 李立, 黄虎兰 . 玉米品种抗旱性鉴定方法与指标研究. 农业现代化研究, 2011,32(1):120-124.
[23] 黄清华, 曹连莆, 张新忠 , 等. 数量性状定位及作物抗旱性分析研究进展. 安徽农业科学, 2007,35(21):6386-6388.
[24] 赵永锋, 王亚卿, 贾晓艳 , 等. 玉米杂交种苗期抗旱性鉴定与评价. 安徽农业科学, 2018,46(9):51-53.
[25] 路之娟 . 不同苦荞品种耐旱性差异鉴定及生理生态特性研究. 临汾:山西师范大学, 2018.
[26] 张永芳, 钱肖娜, 王润梅 , 等. 不同大豆材料的抗旱性鉴定及耐旱品种筛选. 作物杂志, 2019(5):41-45.
[27] 汪灿, 周棱波, 张国兵 , 等. 薏苡种质资源萌发期抗旱性鉴定及抗旱指标筛选. 植物遗传资源学报, 2017,18(5):846-859.
[28] 徐银萍, 潘永东, 刘强德 , 等. 大麦种质资源成株期抗旱性鉴定及抗旱指标筛选. 作物学报, 2020,46(3):448-461.
[1] Zhang Zhizhen, Li Wen, Zhou Qixian, Sun Wei, Zheng Chongke, Xie Xianzhi. Salt Tolerance Evaluation of Different Rice Varieties at Seedling Stage [J]. Crops, 2020, 36(3): 92-101.
[2] Li Jiming,Li Aiguo,Jia Yingquan,Song Congmin,Liu Guihua,Xu Guizhen,Li Heping. Effects of Plant Spacing on Growth and Yield of Oil Sunflower under Mechanized Cultivation Conditions [J]. Crops, 2019, 35(6): 71-75.
[3] Fan Liqin,Li Lei,Wu Xia. Effects of Different Planting Patterns for Oil Sunflower on Saline-Alkali Soil Temperature, Moisture and Electrical Conductivity in Northern Yinchuan Irrigation District [J]. Crops, 2019, 35(6): 127-133.
[4] Wang Yongxing,Shan Feibiao,Yan Wenzhi,Du Ruixia,Yang Qinfang,Liu Chunhui,Bai Lihua. Genetic Diversity Analysis and Code Classification Based on DUS Testing in Sunflower [J]. Crops, 2019, 35(5): 22-27.
[5] Zhang Yongfang,Qian Xiaona,Wang Runmei,Shi Pengqing,Yang Rong. Identification of Drought Resistance of Different Soybean Materials and Screening of Drought Tolerant Varieties [J]. Crops, 2019, 35(5): 41-45.
[6] Yue Linqi,Shi Weiping,Guo Jiahui,Guo Pingyi,Guo Jie. Response of Cutin Synthetic Genes of Foxtail Millet to Drought Stress [J]. Crops, 2019, 35(4): 183-190.
[7] Haiyan Ding,Chunlin Wang,Yan Wu,Guoling Ren,Zuhuan Zhai,Jun Ma. Karyotype Analysis of Chromosome of Sunflower Longkuiza10 [J]. Crops, 2019, 35(2): 99-102.
[8] Yu Bai,Xueying Han,Haiyang Dong,Sudu Wuyun,Bingzhen Li,Guilin Chen. Effects of Rotation and Agronomic Measures on Reducing the Damage of Orobanche cumana [J]. Crops, 2019, 35(2): 192-196.
[9] Shuping Hu,Tiantian Meng,Hui Zhao,Haizhu Bao. Effects of Subsoiling on Photosynthetic Performance and Yield of Sunflower [J]. Crops, 2019, 35(1): 116-120.
[10] Jiao Yuzi,Guo Junmei,Yang Junxing,Li Houen,Xu Tiebing,Ye Yong,Zhou Xiaoyong. Field Study on Variety Difference of Cadmium Accumulation in Sunflower (Helianthus annuus L.) [J]. Crops, 2018, 34(6): 89-95.
[11] Youyu Jia,Xiong Zhang,Yu Gao,Jian Zhang,Yongfeng Ren,Xiulan Yin,Peiyi Zhao,Shengpeng Diao,Jing Nie,Caixia Di,Hao An. Effects of Mulching Ways on Soil Hydro-Thermal Condition and Yield of Sunflowers on Rainfed Farmland [J]. Crops, 2017, 33(6): 72-78.
[12] Shuguang Wang,Yugang Shi,Huawei Shi,Yaping Cao,Daizhen Sun. Research on Relationship between Photosynthetic Characteristics and Drought Resistance in Spring Wheat [J]. Crops, 2017, 33(6): 23-29.
[13] Miao Xu,Wenshou He,Kun Ma. Effects of Chemical and Organic Fertilizers on Yield and Quality of Oilseed Sunflower [J]. Crops, 2017, 33(5): 129-135.
[14] Zhequn Cao,Furong Xiao,Shuying Chen,Lilian He,Fusheng Li. Identification of Cold Tolerance in Seven Wild Sugarcane and Three Offsprings at Seedling Stage [J]. Crops, 2017, 33(5): 43-48.
[15] Guolong Li,Yaqing Sun,Shiqin Shao,Yongfeng Zhang. Response of Antioxidant System to Drought Stress in Sugar Beet Leaves at Seedling Stage [J]. Crops, 2017, 33(5): 73-79.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!