Crops ›› 2023, Vol. 39 ›› Issue (2): 77-82.doi: 10.16035/j.issn.1001-7283.2023.02.011

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Changes of Insect Resistance and Its Correlation Analysis with Yield Traits in Transgenic Bt Cotton from 2005 to 2020

Tang Zhongjie1(), Xie Deyi1(), Xu Shouming2, Nie Lihong1, Lü Shuping1, Wang Mingkun3   

  1. 1Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan,China
    2State Key Laboratory of Cotton Biology, Henan University, Kaifeng 475004, Henan, China
    3Dengzhou Agricultural Technology Extension Center, Dengzhou 474150, Henan, China
  • Received:2021-09-28 Revised:2021-10-09 Online:2023-04-15 Published:2023-04-11

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Abstract:

To provide references for the sustainable use of transgenic Bt cotton in both scientific research and production, this study recorded Yinshan 8 of transgenic Bt cotton for 16 consecutive years since 2005. The results indicated that with the increase of planting-generations, the adjusted mortality of the second, third, and fourth generations of cotton bollworm larvae, and the expression of insecticidal protein of leaves and bolls at bolling stage of Yinshan 8 showed a linear increase in varying degrees, and an increasing trend was also observed for the linear regression of lint yield. The expression of insecticidal protein in leaves at the seedling stage, and in leaves and buds at the bud stage decreased linearly. Correlation analysis suggested that there was a positive correlation between the resistance of insect-resistant cotton and lint yield. The boll number and ginning outturn were important factors constituting the lint yield, which reached extremely significant and significant levels, respectively. The exogenous Bt gene could be stably passed to offspring after being transferred into cotton. Through kanamycin-based identification and systematic selection, the insect-resistance phenotype of transgenic Bt cotton could be maintained for multiple generations. Through purification and rejuvenation, it was even possible to improve the insect resistance of the transgenic Bt cotton in a specific growth period or in certain organs.

Key words: Transgenic Bt cotton, Insect resistance, Yield traits, Correlation analysis

Table 1

Resistance classification standards provided by Institute of Plant Protection, CAAS (2005)"

抗性
Resistance		 等级
Grade		 按室内幼虫3d
校正死亡率
Three days adjusted
mortality by
indoor larva (%)		 按室内幼虫5d
校正死亡率
Five days adjusted
mortality by
indoor larva (%)
高抗High resistance 1 ≥50 ≥70
抗Resistance 2 30~50 40~70
中抗Medium resistance 3 20~30 20~40
低抗Low resistance 4 <20 <20
感Sensitive 5 同非转基因棉花 同非转基因棉花

Fig.1

Trend of calibrated mortality of cotton bollworm larvae of Bt transgenic cotton Yinshan 8 from 2005 to 2020"

Fig.2

Trend of Bt toxin protein expression of transgenic cotton Yinshan 8 from 2005 to 2020"

Fig.3

Yield and traits trend of transgenic cotton Yinshan 8 from 2005 to 2020"

Table 2

Correlation analysis between corrected mortality and yield traits of transgenic Bt cotton bollworm"

项目
Item		 第2代
The second
generation		 第3代
The third
generation		 第4代
The fourth
generation		 株铃数
Bolls number
per plant		 铃重
Boll
weight		 衣分
Ginning
outturn		 皮棉产量
Lint yield
第2代The second generation 1.00
第3代The third generation 0.09 1.00
第4代The fourth generation 0.63** 0.08 1.00
株铃数Bolls number per plant 0.25 0.15 0.39 1.00
铃重Boll weight 0.01 -0.49* -0.25 -0.11 1.00
衣分Ginning outturn 0.14 0.44 0.41 0.35 0.02 1.00
皮棉产量Lint yield 0.24 0.11 0.36 0.94** 0.16 0.54* 1.00
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