Crops ›› 2023, Vol. 39 ›› Issue (3): 260-266.doi: 10.16035/j.issn.1001-7283.2023.03.036

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Analysis of Fungal Diversity in Seeds of Tartary Buckwheat from Liangshan, Sichuan Province

Bai Kaihong(), Abie Xiaobing(), Xu Xiaoli, Jiang Na, Li Jianqiang, Luo Laixin()   

  1. Department of Plant Pathology, China Agricultural University/Key Laboratory of Pest Monitoring and Green Management, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Seed Disease Testing and Control, Beijing 100193, China
  • Received:2021-09-01 Revised:2022-01-11 Online:2023-06-15 Published:2023-06-16

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Abstract:

Tartary buckwheat (Fagopyrum tataricum) is one of the staple food crops in Liangshan Yi Autonomous Prefecture, Sichuan province. The leaf and root diseases caused by pathogenic fungi seriously affected the yield and quality of tartary buckwheat. Pathogen in seeds are important primary infection source of disease. In this study, 46 samples of tartary buckwheat seed from seven counties of Liangshan Prefecture were studied by agar plating method and 18S rRNA amplicon sequencing method. The results showed that a total of 786 fungal isolates and 594 fungal isolates were obtained from inside and outside of seeds by agar plating, respectively, which belonged to 17 genera. Among them, Fusarium and Alternaria were the dominant seed-borne microflora for the inside and outside of the seeds, respectively. A total of 39 genera and 41 genera were identified from inside and outside of seeds by 18S rRNA amplicon sequencing method, respectively. Sclerotinia was the dominant seed-borne microflora for the inside and outside of the tartary buckwheat seeds. Moreover, it was found that different samples from the same area had fungal diversity differences.

Key words: Tartary buckwheat, Fungi, Isolation and identification, Amplicon sequencing

Table 1

The information of tartary buckwheat seeds from Liangshan of Sichuan province"

样品
Sample		 采样地点
Sampling place		 海拔
Altitude
(m)		 测序样品
Sequencing
sample
YX-1 越西县尔赛乡依达村 2394.1 -
YX-2 越西县尔赛乡依达村 2458.4 -
YX-3 越西县尔赛乡依达村 2521.3 -
YX-4 越西县尔赛乡洪洛村 2520.6 -
YX-5 越西县尔赛乡洪洛村 2603.9 -
YX-6 越西县尔赛乡洪洛村 2620.4 -
YX-7 越西县尔赛乡洪洛村 2515.2 +
YX-8 越西县尔赛乡花古村 2489.2 -
YX-9 越西县尔赛乡花古村 2413.6 -
YX-10 越西县尔赛乡花古村 2234.7 -
ZJ-1 昭觉县谷曲乡新凉村 2107.9 +
ZJ-2 昭觉县谷曲乡新凉村 2110.6 -
ZJ-3 昭觉县洒拉地破 2476.6 -
ZJ-4 昭觉县洒拉地破 2589.1 -
ZJ-5 昭觉县洒拉地破 2650.4 +
ZJ-6 昭觉县洒拉地破 2787.5 -
ZJ-7 昭觉县解放沟 2743.2 -
ZJ-8 昭觉县解放沟 2845.6 -
ZJ-9 昭觉县解放沟 2912.3 -
ZJ-10 昭觉县解放沟 3124.7 +
XD-1 喜德县博洛拉达乡博洛村 2795.6 -
XD-2 喜德县博洛拉达乡博洛村 2645.2 -
XD-3 喜德县博洛拉达乡博洛村 2535.3 -
XD-4 喜德县博洛拉达乡火普村 2583.7 -
XD-5 喜德县博洛拉达乡火普村 2454.4 -
XD-6 喜德县博洛拉达乡火普村 2458.1 -
XD-7 喜德县博洛拉达乡博洛村 2448.5 -
XD-8 喜德县博洛拉达乡博洛村 2453.1 -
XD-9 喜德县博洛拉达乡博洛村 2432.5 -
XD-10 喜德县博洛拉达乡博洛村 2408.7 +
MNCQ-1 冕宁县回坪乡庙高山村 2286.4 +
MNCQ-3 冕宁县回坪乡庙高山村 2286.4 -
MNCQ-4 冕宁县回坪乡庙高山村 2286.4 +
PGEL-1 普格县五道箐乡采洛洛博村 2015.6 +
KQ-2008 冕宁县回坪乡庙高山村 2286.4 -
MN1707-893 冕宁县回坪乡庙高山村 2286.4 -
MGYE 美姑县觉罗乡地莫村 2450.6 -
MGQK-6 美姑县觉罗乡地莫村 2450.6 -
MGCQ-2 美姑县觉罗乡地莫村 2450.6 +
MGXQ-2 美姑县觉罗乡地莫村 2450.6 +
MGYQ-2 美姑县觉罗乡地莫村 2450.6 +
YYEL-1 盐源县干海乡滑泥村 2608.2 -
YYYQ-2 盐源县干海乡滑泥村 2608.2 -
YYCQ-3 盐源县干海乡滑泥村 2608.2 +
YY1707-893 盐源县干海乡滑泥村 2608.2 -
YYQK-6 盐源县干海乡滑泥村 2608.2 +

Fig.1

The colony morphology and its spore morphology of tartary buckwheat seeds carrying fungal isolates on PDA medium 1: Botryosphaeria; 2: Bipolaris; 3: Thielavia; 4: Phomopsis; 5: Myrmaecium; 6: Colletotrichum; 7: Phoma; 8: Chaetomium; 9: Pestalotiopsis; 10: Trichoderma; 11: Aspergillus; 12: Penicillium; 13: Lichtheimia; 14: Alternaria; 15: Curvularia; 16: Schizophyllum; 17 and 18: Fusarium. From left to right of each group: the front of colony, the back of colony, and spore morphology, respectively"

Fig.2

Analysis of dominant microflora from tartary buckwheat seeds based on isolation and culture method (a) statistical results of fungal isolates inside of tartary buckwheat seeds; (b) statistical results of fungal isolates outside of tartary buckwheat seeds"

Fig.3

Alpha diversity estimators of 13 tartary buckwheat seeds carrying fungi by 18S rRNA amplicon sequencing (a) statistical results of fungal isolates inside of tartary buckwheat seeds; (b) statistical results of fungal isolates outside of tartary buckwheat seeds"

Fig.4

Community analysis of 13 tartary buckwheat seeds carrying fungi by 18S rRNA amplicon sequencing (a) statistical results of fungal isolates inside of tartary buckwheat seeds; (b) statistical results of fungal isolates outside of tartary buckwheat seeds"

Fig.5

Sample hierarchy clustering analysis of 13 tartary buckwheat seeds carrying fungi by 18S rRNA amplicon sequencing (a) and (c): statistical results of fungal isolates inside of tartary buckwheat seeds; (b) and (d): statistical results of fungal isolates outside of tartary buckwheat seeds; (a) and (b): hierarchical clustering tree; (c) and (d): PCA analysis on OTU level"

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