Crops ›› 2021, Vol. 37 ›› Issue (1): 38-46.doi: 10.16035/j.issn.1001-7283.2021.01.006

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Analysis of SSR Loci in Whole Genome and Development of Molecular Markers in Tartary Buckwheat

Ma Mingchuan(), Liu Longlong, Liu Zhang, Zhou Jianping, Nan Chenghu, Zhang Lijun()   

  1. Center for Agricultural Genetic Resources Research, Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, Shanxi, China
  • Received:2020-04-08 Revised:2021-01-06 Online:2021-02-15 Published:2021-02-23
  • Contact: Zhang Lijun E-mail:mamingchuan6@163.com;15034054161@163.com

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Abstract:

The number of polymorphic SSR markers of tartary buckwheat is limited. In this study, SSRs with 1-6 nucleotide repeats were searched from the published whole genome sequences of tartary buckwheat using MISA software and the characterization of SSR sequences and their polymorphisms were analyzed. A total of 1640 SSR loci were detected in whole genome sequences of tartary buckwheat. The content of tri-nucleotide repeat SSRs was the highest, accounting for 63.29%; the content of penta-nucleotide repeat SSRs was the lowest, only 0.12%. The most abundant repeat motifs were AT/TA, AAG/CTT, ACC/GGT and ATC/GAT. The SSR length ranged from 12 to 476bp, with an average of 23.14bp. The ratio of SSR length ranged from 12 to 19bp was 71.71%, and the SSRs (≥20bp) was 28.29%. Based on the type of SSR loci, 479 pairs of primers were designed and synthesised. The 200 pairs of primers were selected for PCR amplification, 56 of them showed polymorphism, 17 pairs showed polymorphism in five accessions of tartary buckwheat, 48 pairs showed polymorphism in three accessions of common buckwheat, and nine pairs showed polymorphism in tartary buckwheat and common buckwheat. SSR markers can be developed in large-scale based on whole genome sequences of buckwheat and a large number of SSR primers also can be identified for genetic diversity analysis, genetic mapping and variety identification.

Key words: Tartary buckwheat, Whole genome, SSR

Table 1

List of accessions used in accessing SSR level in the tartary buckwheat"

序号No. 资源编号Code 来源地Source 籽粒形状Seed shape 籽粒颜色Seed color 籽粒翅刺Prickly seed 花型Flower type
1 KQ1 山西 短锥 黑色 无 雌雄等长
2 KQ2 西藏 短锥 黑色 无 雌雄等长
3 KQ3 甘肃 长锥 灰色 无 雌雄等长
4 KQ4 青海 长锥 灰色 无 雌雄等长
5 KQ5 山西 短锥 深棕色 有 雌雄等长
6 TQ1 山西 三角形 黑色 无 雌雄异长
7 TQ2 贵州 三角形 黑色 无 雌雄等长
8 TQ3 内蒙古 三角形 褐色 无 雌雄异长

Table 2

Repeat type, number and frequency of SSRs in tartary buckwheat"

重复次数
Repeat number		 重复类型 Repeat type 总计
Total		 比例
Ratio (%)
单核苷酸
Mono-nucleotide		 二核苷酸
Di-nucleotide		 三核苷酸
Tri-nucleotide		 四核苷酸
Quad-nucleotide		 五核苷酸
Penta-nucleotide		 六核苷酸
Hexa-nucleotide
4 0 0 0 0 1 48 49 2.99
5 0 0 667 23 1 13 704 42.93
6 0 106 236 13 0 5 360 21.95
7 0 44 80 13 0 1 138 8.41
8 0 54 21 4 0 1 80 4.88
9 0 26 13 6 0 0 45 2.74
10 0 16 5 6 0 0 27 1.65
11 0 12 1 1 0 0 14 0.85
12 18 10 6 2 0 0 36 2.20
13 10 9 3 1 0 1 24 1.46
14 7 5 1 2 0 0 15 0.91
15 1 4 0 2 0 0 7 0.43
16~25 13 29 5 4 0 0 51 3.11
≥26 5 85 0 0 0 0 90 5.49
总计Total 54 400 1 038 77 2 69 1 640
比例Ratio (%) 3.29 24.39 63.29 4.70 0.12 4.21

Table 3

Occurrence frequency of different microsatellites motifs of tartary buckwheat"

重复类型
Repeat type		 重复基序
Repeat motif		 数目
Number		 重复次数
Repeat number		 平均长度
Mean length (bp)		 比例
Ratio (%)
单核苷酸 A/T 43 12~92 18.74 2.62
Mono-nucleotide C/G 11 12~21 16.18 0.67
二核苷酸 AC/GT 37 6~238 36.27 2.26
Di-nucleotide AG/CT 52 6~12 13.38 3.48
AT/TA 310 6~133 38.67 18.90
三核苷酸 AAC/GTT 104 5~8 16.41 6.34
Tri-nucleotide AAG/CTT 277 5~22 17.48 16.89
AAT/ATT 19 5~16 19.42 1.16
ACC/GGT 199 5~13 21.72 12.13
ACG/CGT 16 5~8 16.5 0.98
ACT/AGT 22 5~9 17.45 1.34
AGC/CTG 80 5~10 16.20 4.88
AGG/CCT 120 5~11 16.25 7.32
ATC/GAT 172 5~14 16.97 10.49
CCG/CGG 29 5~7 16.14 1.77
四核苷酸 ACAT/ATGT 65 5~23 33.72 3.96
Quad-nucleotide AAAG/CTTT,AAAT/ATTT,ACCC/GGGT,
ACCT/AGGT,ACGT/ACGT,ATCC/GGAT		 12 5~10 24.00 0.73
五核苷酸
Penta-nucleotide		 ACAAA,TGGGC 2 4~5 22.50 0.12
六核苷酸
Hexa-nucleotide		 AAAAAG/CTTTTT,AAACCC/GGGTTT,…,
AGGCGG/CCGCCT,CCCCGG/CCGGGG（共48种）		 69 4~13 27.39 4.21

Fig.1

Frequency of SSRs with different length in tartary buckwheat genome"

Fig.2

Variation in sequence length of different repeat types of SSR"

Table 4

Polymorphic analysis for PCR amplicons of 56 SSR primer pairs in eight buckwheat genotypes"

引物编号
Primer No.		 引物序列（5′-3′）
Primer sequence		 SSR基元
SSR motif		 产物长度
Length of
product (bp)		 多态性Polymorphism
5份苦荞
Five tarary buckwheats		 3份甜荞
Three common buckwheats
P7 F:CAAGGTTCATGTAGTAACCGGAG
R: CTGCTTGGTTGATGTTGATGATA		 (AAC)5 119 无 有
P13 F: GGATACCTTCATCTCTTTCGACC
R: TAGTTGTTATTGGAGAAGCAGGG		 (AAC)5 111 无 有
P18 F: CTCAAATCCAGAACTCACTTGCT
R: GCCACGACGTCAATATACTCTTC		 (GAT)6 156 无 有
P21 F: CATCACCACCAGTTCATAAGCC
R: TCCCTCCATTAAAGAACACCATA		 (TGC)6 160 有 无
P27 F: ATAGACACATCTCTGACACCACG
R: ATCATCTTCTTCCATCTTGCTGA		 (GAA)7 152 无 有
P29 F: CGGAAACAACAGCACAAGAGTAT
R: AGGCTATCGCTACTGCTGACTC		 (TAG)6 83 有 无
P30 F: ACCAATCAACCATTTCTTACCCT
R: TAGCTTTGGAGACTTCATCTTCG		 (ACA)7 143 无 有
P31 F: TACAATCTTCCTCCTCAAGGTCA
R: ATTGCTTGGGATTGTTGAAGTAG		 (ACC)6 152 有 有
P34 F: TAGGAGGAAGACCGTTTATAGGC
R: TCATTCCAAAGACTGCTTGAATC		 (CCA)9 156 有 有
P37 F: ATCCGAGGATCACAAAAATCATA
R: GGCAAAGAATCATTTAAAGCAAA		 (GAT)5 135 无 有
P39 F: TACTGGACAGATCATTCAGCTTG
R: TGATGATTCTGATTCTGATGGTG		 (TCACCC)4 138 有 有
P43 F: CACATAAGCTGAAGTCTACGCCT
R: GGTGTTAGGTCACCAGATGAAGA		 (CCTCCA)5 136 无 有
P46 F: ACCAACTGCCAGAGACCATACTA
R: GGAGTATGTAAGCCGAATATCCC		 (AAG)5 138 无 有
P54 F: TCATATTTGGAATTGTGGAGAGG
R: CCATTCATCAAAGGATGACCTAC		 (TGG)5 126 无 有
P58 F: TCCTGCTGCTTCTTACCAAACTA
R: GCTTGCCCTACATGCATTAAGTA		 (ACA)5 132 无 有
P59 F: ACTGTCAAATTTCAGGTCGAAAA
R: GTCTCCGGTTCTCTTTCCTAATC		 (TCA)6 142 有 无
P62 F: GCTCCTCTAGCTCTTCTAGCGAT
R: TCAACGACTTCCACTTTCTCTTC		 (AGA)5 151 有 有
P66 F: ATTACTAGTCTTCTTCGTCGCCC
R: ATGTTATTGTCGTCCAGAGAAGG		 (CT)7 143 有 无
P67 F: GGTTGCATTGAAGTCTATTGAGG
R: TCAGATTCTTCTTCCAACACCTT		 (AAG)6 159 无 有
P68 F: AAGAGCAAAAAGAAATTGCAAGA
R: AGCTGGTGTCATCAAGATCAGTT		 (GAT)5 145 无 有
P75 F: CCGAATATCAACAATTTCTCTCG
R: GACGTTGAATATTGGAGTGAAGG		 (TTC)5 137 无 有
P78 F: TCTTCAGCAACATCAAAGATCAA
R: ATCTTGCCCTTCAATATGGATTT		 (ATC)5 123 无 有
P79 F: TTATAAGTCACCACCACCTCCAG
R: TGGGTACTCCCAGTCATAACATC		 (CAC)7 154 无 有
P82 F: TTCAGATGGCACTAATGATGATG
R: GTTGCTTATGTTTCTTTGCATCC		 (ATG)5 134 无 有
P86 F: AAGAAAAGGATTCATGGTTGGAT
R: GAGCATCAGCTATACGCCTCTTA		 (GAA)6 159 无 有
P88 F: CTCTTCCTCTAGGATTTCTCGGA
R: AAGGATTTTCTTCGGTTGTTGAC		 (ACC)6 93 有 无
P94 F: AGCTGAAGCAGTTTCTACACACC
R: AAGCCTCATTCTCATTCTCCTTC		 (AATGAG)5 87 有 有
P105 F: ATTGGGTAAATGTGGTGTTGAAG
R: CTTGGGAGATTCCTTCTTGTTTT		 (GAA)5 128 无 有
P108 F: ATCATTTTGTTGATAGCCCAGAT R: TACTGCAATGGTTGTGTTTGTGT (CAACAC)4 137 无 有
P110 F: CCACACCAAAGTATGAAATGGTT
R: GAAATGATCTCATGCACATACCA		 (GAT)5 157 无 有
P114 F: GATGACGGAGAAGATATCAGCAG
R: TTCTTCATCATAAAAGAGGGCTG		 (GCA)6 156 无 有
P116 F: AGCTACAACAAGGTGGTATCCAA
R: GAGAAGACTGCAACTGAGCTGAT		 (CAA)5 108 无 有
P117 F: ATCTCAATCTTCTCTGCAGCAAC
R: TGGGATATTAGTTTGTTGAGCCA		 (CAA)5 154 有 有
P118 F: ATCCTGGCATAAAAGATTGGATT
R: TCCTTTTCTGGCTTGATGTAAAC		 (AT)6 83 无 有
P121 F: CTCATTCTCCTCTTTTGCCTCTT
R: GTCCTTAGAAGAGGACGTTCCAG		 (GGA)5 156 无 有
P124 F: GTGAGGATTGGTCTGACATTGAT
R: GATCCCAGTTTCACACTATCACC		 (GAT)7 151 无 有
P125 F: GACGAAATTGACCTTCTTCCAC
R: AGAAGTGGACGTTTCTTCATCAC		 (AGG)5 141 无 有
P127 F: GCTCAAATCCACAAACAATTTTC
R: CAGTAACGAGGAACGGAGAATC		 (GAG)6 138 无 有
P130 F: ACACCATCTCCTCCAACAGAAAC
R: CAAGCGTCTAGCTTTAAGGTGTC		 (CCA)5 113 无 有
P135 F: ATGAAGGTAGAACGTGGTATGGA
R: GCTTCTTCAACAATGGAGTCAGT		 (GAT)5 160 无 有
P143 F: TGCTTTGCTTGACTCTGATGATA
R: GTGAGCTTGTAACTTCCTCCTCA		 (TGA)5 125 有 有
P144 F: GCTAAAGACAATCAAGAGACGGA
R: TAGGATTTCAACCCTCATCATGT		 (AAC)5 133 无 有
P145 F: GGGCATAAGCGATCATACAATAG
R: TTGAGTTTCCTTCATGGTTCAAG		 (ATG)5 89 无 有
P149 F: GTAGCTCTACAACAACAGGCACC
R: GCCATGGTGGTTACCAGAAGTAT		 (CAA)5 152 有 有
P158 F: CAACAACTCATGTGATTGGAAAA
R: TTGATCCCTATAAGATCCTGCAA		 (CAA)5 147 无 有
P159 F: TGGCAGTGAGTCACAGAGTAATG
R: CACGCAAGAATTTTTCAATAAGG		 (GAT)7 148 无 有
P160 F: ATCATCCCCACTTGTCAACTCT
R: GTATCATCCTGGGACTGAGACTG		 (TCTCAG)4 93 有 无
P162 F: GAAAAGGCAGCAGTAAATTCTGA
R: AAGCTATTGGATTTTCTACCCCA		 (CAT)5 155 无 有
P163 F: TCTATACTTGGTGAGGAGTCGGA
R: GTCCCTTATAGCCATTTGCTCTT		 (GAT)6 129 有 有
P171 F: GAGAGAAAGGGGAGAGGAAGAGT
R: CAAACATCTTGAACCTCCAAAAG		 (AAG)5 103 无 有
P179 F: GATGTCTCGAATGATTGTGATGA
R: TGTCATCCTGAGTTTTCTTCGTT		 (AAG)5 115 无 有
P183 F: TGTCCATATTAGACCCACCATTC
R: AGATGTTGCTTTGCTCTTTGAAC		 (GAA)6 127 无 有
P192 F: AACCATCACTTCCTGAAAGAACA
R: CTGAGCCTCGAGATAGAATTTCA		 (AGA)5 84 有 无
P198 F: ATCGGACTCTCCTAAGTCATCG
R: GTCATCATCGGTAGATGGACTGT		 (CTC)5 145 无 有
P199 F: TGATCATGGATCTGGTTATTCAA
R: TAAACCCGATTTCCAAACTTCTC		 (GAG)6 114 无 有
P200 F: TTATGGCTCTGAAAGAGAAGGAA
R: CTACGCAAACAAATGTCATTCAG		 (GAA)5 139 有 无

Fig.3

Polymorphisms of primer 34 and primer 39 in five tartary buckwheat germplasms and three common buckwheat germplasms"

[1] 胡银岗, 冯佰利, 周济铭, 等. 荞麦遗传资源利用及其改良研究进展. 西北农业学报, 2005,14(5):101-109.
[2] 张丽君, 马名川, 刘龙龙, 等. 山西省苦荞品种资源研究. 河北农业科学, 2015,19(1):69-74.
[3] 李秀莲, 史兴海, 朱慧珺. 苦荞产品开发应用现状及发展对策. 山西农业科学, 2011,39(8):908-910.
[4] 王玲玲, 陈东亮, 黄丛林, 等. SSR分子标记技术在植物研究中的应用. 安徽农业科学, 2017,45(36):：123,126,130.
[5] 杨梦婷, 黄洲, 干建平, 等. SSR分子标记的研究进展. 杭州师范大学学报(自然科学版), 2019,18(4):429-436.
[6] Iwata H, Imon K, Tsumura Y, et al. Genetic diversity among Japanese indigenous common buckwheat (Fagopyrum esculentum) cultivars as determined from amplified fragment length polymorphism and simple sequence repeat markers and quantitative agronomic traits. Genome, 2005,48(3):367-377.
doi: 10.1139/g04-121 pmid: 16121234
[7] Konishi T, Yasui Y, Ohnishi O. Original birthplace of cultivated common buckwheat inferred from genetic relationships among cultivated populations and natural populations of wild common buckwheat revealed by AFLP analysis. Genes and Genetic Systems, 2005,80(2):113-119.
doi: 10.1266/ggs.80.113 pmid: 16172523
[8] 候雅君, 张宗文, 吴斌. 苦荞种质资源AFLP标记遗传多样性分析. 中国农业科学, 2009,42(12):4166-4174.
[9] 王莉花, 殷富有, 刘继梅. 利用RAPD分析云南野生荞麦资源的多样性和亲缘关系. 分子植物育种, 2004,2(6):807-815.
[10] Tsuji K, Ohnishi O. Origin of cultivated tartary buckwheat (Fagopyrum tataricum Gaertn.) revealed by RAPD analyses. Genetic Resources and Crop Evolution, 2000,47:431-438.
doi: 10.1023/A:1008741513277
[11] Kump B, Javornik B. Genetic diversity and relationships among cultivated and wild accessions of tartary buckwheat (Fagopyrum tataricum Gaertn.) as revealed by RAPD markers. Genetic Resources and Crop Evolution, 2002,49(6):565-572.
doi: 10.1023/A:1021250300572
[12] 邓琳琼, 张奎, 黄凯丰, 等. 甜荞和苦荞品种遗传多样性的RAPD分析. 安徽农业科学, 2011,39(15):8895-8898.
[13] Li Y Q, Fan X L, Shi T L, et al. SRAP marker reveals genetic diversity in tartary buckwheat in China. Frontiers of Agriculture in China, 2009,3(4):383-387.
doi: 10.1007/s11703-009-0071-5
[14] Konishi T, Iwata H, Yashiro K, et al. Development and characterization of microsatellite markers for common buckwheat. Breed Science, 2006,56:277-285.
doi: 10.1270/jsbbs.56.277
[15] Ma K H, Kim N S, Lee G A, et al. Development of SSR markers for studies of diversity in the genus Fagopyrum. Theoretical and Applied Genetics, 2009,119:1247-1254.
doi: 10.1007/s00122-009-1129-8 pmid: 19680622
[16] Li Y Q, Shi T L, Zhang Z W. Development of microsatellite markers from tartary buckwheat. Biotechnology Letters, 2007,29(5):823-827.
doi: 10.1007/s10529-006-9293-2 pmid: 17216537
[17] 高帆, 张宗文, 吴斌. 中国苦荞SSR分子标记体系构建及其在遗传多样性分析中的应用. 中国农业科学, 2012,45(6):1042-1053.
doi: 10.3864/j.issn.0578-1752.2012.06.002
[18] 韩瑞霞, 张宗文, 吴斌. 苦荞SSR引物开发及其在遗传多样性分析中的应用. 植物遗传资源学报, 2012,13(5):759-764.
[19] 石桃雄, 黎瑞源, 郭菊卉, 等. 基于普通荞麦种子表达序列标签微卫星标记的开发. 贵州农业科学, 2014,42(3):1-5.
[20] 黎瑞源, 潘凡, 陈庆富, 等. 苦荞转录组EST-SSR发掘及多态性分析. 中国农业科技导报, 2015,17(4):42-52.
[21] 杜晓磊, 张宗文, 吴斌, 等. 苦荞SSR分子遗传图谱的构建及分析. 中国农学通报, 2013,29(21):61-65.
[22] 黎瑞源, 梁龙兵, 石桃雄, 等. 苦荞重组自交系群体F5代SSR遗传图谱的构建. 贵州师范大学学报(自然科学版), 2017,35(4):31-45.
[23] 莫日更朝格图, 王鹏科, 高金锋, 等. 苦荞地方种质资源的遗传多样性分析. 西北植物学报, 2010,30(2):255-261.
[24] 田晓庆, 徐宏亚, 汪灿, 等. 用SSR标记分析荞麦栽培种质资源的遗传多样性. 作物杂志, 2013(5):28-32.
[25] 杨学文, 丁素荣, 胡陶, 等. 104份苦荞种质的遗传多样性分析. 作物杂志, 2013(6):13-17.
[26] 徐笑宇, 方正武, 杨璞, 等. 苦荞遗传多样性分析与核心种质筛选. 干旱地区农业研究, 2015,33(1):268-277.
[27] Zhang L, Li X, Ma B, et al. The tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance. Molecular Plant, 2017,10(9):1224-1237.
pmid: 28866080
[28] Varshney R K, Graner A, Sorrells M E. Genic microsatellite markers in plants:features and applications. Trends in Biotechnology, 2005,23(1):48-55.
pmid: 15629858
[29] Lawson M J, Zhang L Q. Distinct patterns of SSR distribution in the Arabidopsis thaliana and rice genomes. Genome Biology, 2006,7(2):R14.1-R14.11.
[30] 原志敏. 玉米全基因组 SSRs 分子标记开发与特征分析. 雅安:四川农业大学, 2013.
[31] Kantety R V, Rota M L, Matthews D E, et al. Data mining for simple sequence repeats in expressed sequence tags from barley,maize,rice,sorghum and wheat. Plant Molecular Biology, 2002,48:501-510.
pmid: 11999831
[32] 王玉龙, 黄冰艳, 王思雨, 等. 四倍体野生种花生A. monticola全基因组SSR的开发与特征分析. 中国农业科学, 2019,52(15):2567-2580.
doi: 10.3864/j.issn.0578-1752.2019.15.002
[33] 李海洋, 李荣华, 夏岩石, 等. 基于RAD-seq数据开发烟草多态性SSR标记. 中国烟草科学, 2018,39(1):1-9.
[34] Song Q, Jia G, Zhu Y, et al. Abundance of SSR motifs and development of candidate polymorphic SSR markers (BARCSOYSSR1.0) in soybean. Crop Science, 2010,50(5):1950-1960.
[35] 郑燕, 张耿, 吴为人. 禾本科植物微卫星序列的特征分析和比较. 基因组学与应用生物学, 2011,30(5):513-520.
[36] Morgante M, Hanafey M, Powell W. Microsatellites are preferentially associated with nonrepetitive DNA in plant genomes. Nature Genetics, 2002,30(2):194-200.
doi: 10.1038/ng822 pmid: 11799393
[37] Dreisigacker S, Zhang P, Warburton M L, et al. SSR and pedigree analyses of genetic diversity among CIMMYT wheat lines targeted to different mega environments. Crop Science, 2004,44(2):381-388.
[38] Temnykh S, DeClerck G, Lukashova A, et al. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.):Frequency,length variation,transposon associations,and genetic marker potential. Genome Research, 2001,11(8):1441-1452.
doi: 10.1101/gr.184001 pmid: 11483586
[39] 陈仕勇, 马啸, 张新全, 等. 不同来源SSR和EST-SSR在披碱草属和鹅观草属物种中的通用性分析. 草业学报, 2016,25(2):132-140.
doi: 10.11686/cyxb2015324
[40] 卢玉飞, 蒋建雄, 易自力. 玉米SSR引物和甘蔗EST-SSR引物在芒属中的通用性研究. 草业学报, 2012,21(5):674-679.
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[10] . [J]. Crops, 2011, 27(6): 10 -13 .